Apolipoprotein J / Apo J antibody

Principal name

Apolipoprotein J / Apo J antibody

Alternative names for Apolipoprotein J / Apo J antibody

Clusterin, APOJ, CLI, KUB1, AAG4, NA1/NA2, SP-40,40, Complement-associated protein SP-40,40, Complement cytolysis inhibitor, Testosterone-repressed prostate message 2, Ku70-binding protein 1, Aging-associated gene 4 protein

SwissProt ID

P05371 (Rat), P10909 (Human), P17697 (Bovin), P25473 (Canfa), Q06890 (Mouse), Q29549 (Pig), Q9XSC5 (Rabit)

Gene ID

1191 (CLU), 12759 (Clu), 24854

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Goat, Mouse

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Flow Cytometry (F), Frozen Sections (C), Immunoprecipitation (IP), ELISA (detection) (E(detection))

Background of Apolipoprotein J / Apo J antibody

Clusterin is a 75-80 kD disulfide-linked heterodimeric protein containing about 30% of N-linked carbohydrate rich in sialic acid, but truncated forms targeted to the nucleus have also been identified. The precursor polypeptide chain is cleaved proteolytically to remove the 22-mer secretory signal peptide and subsequently between residues 227/228 to generate the alpha and beta chains. These are assembled antiparallel to give a heterodimeric molecule in which the cysteine-rich centers are linked by five disulfide bridges and are flanked by two predicted coiled-coil alpha-helices and three predicted amphipathic alpha-helices. The six sites of N-linked glycosylation are indicated as yellow spots. Across a broad range of species clusterin shows 70% to 80% of sequence homology. It is ubiquitously expressed in most mammalian tissues and can be found in plasma, milk, urine, cerebrospinal fluid and semen. It is able to bind and form complexes with numerous partners such as immunoglobulins, lipids, heparin, bacteria, complement components, paraoxonase, beta amyloid, leptin and others. Clusterin has been ascribed a plethora of functions such as phagocyte recruitment, aggregation induction, complement attack prevention, apoptosis inhibition, membrane remodelling, lipid transport, hormone transport and/or scavenging, matrix metalloproteinase inhibition. A detailed mechanism of clusterin has not been defined. One tempting hypothesis says that clusterin is an extracellular chaperone protecting cells from stress induced by degraded and misfolded protein precipitates.Clusterin is up- or downregulated on the mRNA or protein level in many pathological and clinically relevant situations including cancer, organ regeneration, infection, Alzheimer disease, retinitis pigmentosa, myocardial infarction, renal tubular damage, autoimmunity and others.

General readings

Maximino R et al., (2000) Am J Pathol 157(2):393?399
Ingrid C.G et al., (1998) Biochem J 331:231?237
Maximino R et al., (1990) J Clin Invest 85:1477?1486
Fabien L et al., (2006) Biochem J 395(1):223?231

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Apolipoprotein J / Apo J antibody

Apolipoprotein J / Apo J Mouse IgG1 Hs-3 Purified Hu C, E, ICC/IF, WB
0.1 mg / €370.00
  Acris Antibodies GmbH

Apolipoprotein J / Apo J antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-CLU rabbit polyclonal antibody. (TA590103) Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Apolipoprotein J / Apo J antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-CLU mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808665)(1:150) Mouse IgG2a OTI5D3 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Apolipoprotein J / Apo J antibody

Immunohistochemistry: AM09004PU-N Clusterin antibody staining of Formalin-Fixed, Paraffin-Embedded Human Prostate at 10 µg/ml followed by biotinylated anti-Mouse IgG secondary antibody, Alkaline Phosphatase-Streptavidin and chromogen. Mouse IgG1 1A11 Purified Hu E, F, ICC/IF, P, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

Apolipoprotein J / Apo J antibody

Immunohistochemistry: AM09004PU-N Clusterin antibody staining of Formalin-Fixed, Paraffin-Embedded Human Prostate at 10 µg/ml followed by biotinylated anti-Mouse IgG secondary antibody, Alkaline Phosphatase-Streptavidin and chromogen. Mouse IgG1 1A11 Purified Hu E, F, ICC/IF, P, WB
50 µl / €230.00
  Acris Antibodies GmbH

Apolipoprotein J / Apo J (312-325) antibody

Staining of Mouse Testis lysate (35µg protein in RIPA buffer) using APOJ Antibody Cat.-No AP31850PU-N at 0.3 µg/ml. Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Ms, Rt E, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Apolipoprotein J / Apo J (44-58) antibody

AP32025PU-N Apolipoprotein J Antibody staining of Human Prostate lysate at 0.03 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Apolipoprotein J / Apo J antibody

TA303058 (0.3µg/ml) staining of human colon lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Apolipoprotein J / Apo J (C-term) antibody

TA305888 (0.3ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Apolipoprotein J / Apo J antibody

Western blot analysis of Clusterin in human brain tissue lysate with Clusterin body at (A) 0.5 and (B) 1 ug/ml. Rabbit IgG Aff - Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Apolipoprotein J / Apo J antibody

Predicted band size: 53 kDa . Positive control: Y79 cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Apolipoprotein J / Apo J antibody

Western blot analysis of extracts of various cell lines, using CLU antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Apolipoprotein J / Apo J antibody

equine cartilage explants Rabbit IgG Purified Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Apolipoprotein J / Apo J antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CLU (RC203941, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CLU.(1:2000) Mouse IgG2a OTI2A7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Apolipoprotein J / Apo J antibody

Apolipoprotein J / Apo J Mouse IgG1 3R3/2 Purified Hu C, E, F, WB
0.1 mg / €480.00
  Acris Antibodies GmbH

Apolipoprotein J / Apo J antibody

AM03078PU-N Apolipoprotein J antibody staining of  Formalin-Fixed Paraffin-Embedded Human Testis at 10 µg/ml. Mouse IgG1 Hs-3 Aff - Purified Hu E, ICC/IF, P, WB
0.1 mg / €240.00
  Acris Antibodies GmbH

Apolipoprotein J / Apo J antibody

Western blot analysis of Clusterin in human brain tissue lysate with Clusterin body at (A) 0.5 and (B) 1 µg/ml. Rabbit IgG Purified Hu WB
0.1 mg / €370.00
  Acris Antibodies GmbH

Apolipoprotein J / Apo J (Alpha) antibody

Immunohistochemistry (IHC) analyzes of Clusterin-alpha antibody (Cat.-No.: AP20482PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Apolipoprotein J / Apo J (488-501) antibody

Human Testis (formalin-fixed, paraffin-embedded) stained with CLU antibody AP22475PU-N at 2.5 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Goat Aff - Purified Can, Eq, Hst, Hu, Mky, Prim, Rb E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Apolipoprotein J / Apo J (N-term) antibody

CLU antibody (N-term) (Cat. #AP50979PU-N) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CLU antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

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