Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Flow Cytometry (F), Paraffin Sections (P)
Background of Apolipoprotein L6 / ApoL6 antibody
This gene is a member ofThe apolipoprotein L gene family.The encoded protein is found inThe cytoplasm, where it may affectThe movement of lipids or allowThe binding of lipids to organelles. [provided by RefSeq, Jul 2008].
Liu Z, Lu H, Jiang Z, Pastuszyn A, Hu CA. Apolipoprotein l6, a novel proapoptotic Bcl-2 homology 3-only protein, induces mitochondria-mediated apoptosis in cancer cells. Mol Cancer Res. 2005 Jan;3(1):21-31. PMID: 15671246
GTX47580 (2µg/ml) staining of Human Skeletal Muscle lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Human COLO205; WB Suggested Anti-APOL6 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: COLO205 cell lysate. APOL6 is supported by BioGPS gene expression data to be expressed in COLO205.; APOL6 antibody - middle region (ARP36054_P050) in Human COLO205 cells using Western Blot
APOL6 Antibody (Center) (Cat. #AP50213PU-N) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the APOL6 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
APOL6 Antibody (Center) (Cat. #AP50213PU-N) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.