Apolipoprotein M / Apo M antibody
Principal name
Apolipoprotein M / Apo M antibody
Alternative names for Apolipoprotein M / Apo M antibody
Apo-M, ApoM, Protein G3A, NG20, HSPC336
Ncbi ID
9606, NP_061974, NP_061974.2, NM_019101, NP_061286.1, NM_018816, NM_019373
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of Apolipoprotein M / Apo M antibody
ApoM (Apolipoprotein M, also designated G3a or NG20), with 188-amino acid protein(about 21kDa), is an apolipoprotein and member of the lipocalin protein family. The Apo-proteins are involved in the specific binding of cellular receptors, the regulation of lipolytic enzymes, and the process of lipid exchange. The encoded protein is secreted through the plasma membrane but remains membrane-bound, where it is involved in lipid transport. The N-terminal region of Apo-M contains hydrophobic residues that may promote association with the phospholipid layer of lipoprotein particles. In vitro, Apo-M is glycosylated when translated in the presence of microsomes, and remains associated with the microsomes after carbonate treatment. Apo-M is expressed in liver and kidney, and is secreted into the bloodstream in HDLs, and also found in triglyceride-rich lipoproteins and LDLs.
General readings
1. Xu N, Nilsson-Ehle P, Ahren B.
Correlation of apolipoprotein M with leptin and cholesterol in normal and obese subjects.
J Nutr Biochem. 2004 Oct;15(10):579-82.
PMID: 15542348
Western Blot analysis of APOM expression in transfected 293T cell line by APOM monoclonal antibody (M01), clone 1F10.Lane 1: APOM transfected lysate(21.3 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of APOM over-expressed 293 cell line, cotransfected with APOM Validated Chimera RNAi ( Cat # H00055937-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with APOM monoclonal antibody (M01), clone 1F10 (Cat # H00055937-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Detection limit for recombinant GST tagged APOM is approximately 0.3ng/ml as a capture antibody.
Detection limit for recombinant GST tagged APOM is approximately 0.3ng/ml as a capture antibody.
APOM polyclonal antibody ( Cat # PAB7182 ) ( 5 µg/ml ) staining of paraffin embedded human liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot analysis of APOM expression in transfected 293T cell line (H00055937-T01) by APOM MaxPab polyclonal antibody.Lane 1: APOM transfected lysate(21.30 KDa).Lane 2: Non-transfected lysate.
Recombinant human APOM, 23-188 aa, His-tagged: 15% SDS-PAGE (3 µg)
Immunofluorescence analysis of methanol-fixed L-02 (left) and Cos7 (right) cells using ApoM antibody Cat.-No AM06157PU-N showing cytoplasmic and membrane localization.
Western blot analysis using ApoM antibody Cat.-No AM06157PU-N against Human Serum (1, 2).
Immunofluorescence analysis of methanol-fixed L-02 (left) and Cos7 (right) cells using ApoM antibody Cat.-No AM06158PU-N showing cytoplasmic and membrane localization.
Western blot analysis using ApoM antibody Cat.-No AM06158PU-N against GST-ApoM recombinant protein (1) and human serum (2).
Immunohistochemical staining of paraffin-embedded liver within the normal limits using anti-APOM (TA500074) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
Immunohistochemical staining of paraffin-embedded colon within the normal limits using anti-APOM (TA500074) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
Immunohistochemical staining of paraffin-embedded Carcinoma of liver using anti-APOM (TA500074) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY APOM (RC204679) (RC204679, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-APOM.
TA303181 (5µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
TA305872 (0.5ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot analysis of extracts of various cell lines, using APOM antibody.
Immunohistochemistry of paraffin-embedded human thyroid cancer using APOM antibody at dilution of 1:200 (200x lens).
Immunohistochemistry of paraffin-embedded human endometrial cancer using APOM antibody at dilution of 1:200 (200x lens).
Immunohistochemistry of paraffin-embedded mouse lung using APOM antibody at dilution of 1:200 (200x lens).
Gel: 12+15%SDS-PAGE, Lysate: 40 ug, Lane 1-3: Human fetal liver tissue, Human liver cancer tissue, human placenta tissue, Primary antibody: (APOM Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute
- Quick-Filters:
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA332619 | Apolipoprotein M / Apo M antibody |
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| Rabbit anti-APOM Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | P, WB |
0.1 mg /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody! |
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| TA500074 | Apolipoprotein M / Apo M antibody |
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| anti-APOM (Apolipoprotein M) mouse monoclonal antibody, clone OTI3H3 (formerly 3H3) | |||||||
 |
Mouse | IgG2b | OTI3H3 | Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Highly validated antibody!
|
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| TA303181 | Apolipoprotein M / Apo M (C-term) antibody |
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| Goat Polyclonal Antibody against APOM | |||||||
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Goat | Aff - Purified | Can, Hu | E, P |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA305872 | Apolipoprotein M / Apo M antibody |
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| Goat Anti-Apolipoprotein M (mouse) Antibody | |||||||
 |
Goat | Aff - Purified | Hu | E, WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA349487 | Apolipoprotein M / Apo M antibody |
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| Rabbit Polyclonal Anti-APOM Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AM06157PU-N | Apolipoprotein M / Apo M antibody |
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 |
Mouse | IgG1 | 8F12C6B8 | Purified | Hu | ICC/IF, WB |
0.1 ml /
€440.00
|
| Acris Antibodies GmbH | |||||||
| +1 additional image |
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| AM06158PU-N | Apolipoprotein M / Apo M antibody |
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 |
Mouse | IgG2a | 10C3G5 | Purified | Hu | ICC/IF, WB |
0.1 ml /
€440.00
|
| Acris Antibodies GmbH | |||||||
| +1 additional image |
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CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN301437 | Apom - mouse gene knockout kit via CRISPR |
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Mouse | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN301437BN | Apom - mouse gene knockout kit via CRISPR |
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|
Mouse | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN301437LP | Apom - mouse gene knockout kit via CRISPR |
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|
Mouse | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN301437RB | Apom - mouse gene knockout kit via CRISPR |
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Mouse | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP304679 | Apolipoprotein M / Apo M |
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| Recombinant protein of human apolipoprotein M (APOM) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€680.00
|
|
| OriGene Technologies, Inc. | |||||
| AR09421PU-L | Apolipoprotein M / Apo M (23-188, His-tag) |
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Human | Purified | > 90 % by SDS – PAGE | E. coli |
0.25 mg /
€1,000.00
|
| Acris Antibodies GmbH | |||||
| +1 additional image |
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| AR09421PU-N | Apolipoprotein M / Apo M (23-188, His-tag) |
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|
Human | Purified | > 90 % by SDS – PAGE | E. coli |
50 µg /
€370.00
|
| Acris Antibodies GmbH | |||||
| +1 additional image |
|||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY402735 | APOM overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC204679L2V | Lenti ORF particles, APOM (mGFP-tagged) - Human apolipoprotein M (APOM), 200 uL, >10^7 TU/mL |
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200 µl /
€907.00
|
|
| OriGene Technologies, Inc. | ||
| RC204679L1V | Lenti ORF particles, APOM (Myc-DDK tagged) - Human apolipoprotein M (APOM) , 200 uL, >10^7 TU/mL |
|
|
200 µl /
€804.00
|
|
| OriGene Technologies, Inc. | ||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR201811L2V | Lenti ORF particles, Apom (GFP-tagged) - Mouse apolipoprotein M (Apom), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€907.00
|
|
| OriGene Technologies, Inc. | ||
| MR201811L1V | Lenti ORF particles, Apom (Myc-DDK-tagged) - Mouse apolipoprotein M (Apom), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€804.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR204974L1V | Lenti ORF particles, Apom (Myc-DDK-tagged ORF) - Rat apolipoprotein M (Apom), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€804.00
|
|
| OriGene Technologies, Inc. | ||
