APPL1 / DIP13A antibody

Principal name

APPL1 / DIP13A antibody

Alternative names for APPL1 / DIP13A antibody

Dip13-alpha, KIAA1428, DCC-interacting protein 13-alpha, Adapter protein containing PH domain, PTB domain and leucine zipper motif 1

SwissProt ID

Q8K3H0 (Mouse), Q9UKG1 (Human)

Gene ID

26060 (APPL1), 72993 (Appl1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Paraffin Sections (P)

Background of APPL1 / DIP13A antibody

Kit Component:
- KN301444G1, Appl1 gRNA vector 1 in pCas-Guide vector
- KN301444G2, Appl1 gRNA vector 2 in pCas-Guide vector
- KN301444D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

1. Mao X, Kikani CK, Riojas RA, Langlais P, Wang L, Ramos FJ, Fang Q, Christ-Roberts CY, Hong JY, Kim RY, Liu F, Dong LQ. APPL1 binds to adiponectin receptors and mediates adiponectin signalling and function. Nat Cell Biol. 2006 May;8(5):516-23. Epub 2006 Apr 16. Erratum in: Nat Cell Biol. 2006 Jun;8(6):642.
PMID: 16622416

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

APPL1 / DIP13A antibody

TA302413 (0.5µg/ml) staining of 1) Rat Aortic Smooth Muscle cell lysate 2)  after infection with Human APPL1 adenovirus for 48hrs.  Detected by chemiluminescence.  Data kindly provided by Dr. L. Jia, Ann Arbor, USA. Goat Aff - Purified Bov, Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

APPL1 / DIP13A antibody

Predicted band size: 80 kDa . Positive control: A172 cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/600 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 40 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

APPL1 / DIP13A antibody

Predicted band size: 80 kDa . Positive control: SKOV3 and K562 cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lane1: SKOV3 cell lysate Lane2: K562 cell lysate Lysates: 40 ug per lane Primary antibody: 1/275 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 3 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

APPL1 / DIP13A antibody

Western blot analysis of extracts from A549 cells, using APPL1 antibody. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

APPL1 / DIP13A antibody

WB Suggested Anti-APPL1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: THP-1 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

APPL1 / DIP13A (C-term) antibody

APPL1 / DIP13A Goat Aff - Purified Hu, Ms E, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

APPL1 / DIP13A antibody

APPL polyclonal antibody (A01), Lot # HRI0060508QCS1. Western Blot analysis of APPL expression in NIH/3T3. Mouse Serum Hu, Ms E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

APPL1 / DIP13A antibody

APPL1 / DIP13A Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

APPL1 / DIP13A antibody

APPL1 / DIP13A Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

APPL1 / DIP13A antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between APPL1 and PIK3CA HeLa cells were stained with anti-APPL1 rabbit purified polyclonal 1:1200 and anti-PIK3CA mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

APPL1 / DIP13A antibody

APPL1 polyclonal antibody ( Cat # PAB7191 ) ( 0.5 µg/ml ) staining of A ) rat aortic smooth muscle cell lysate B ) after infection with human APPL1 adenovirus for 48hrs. Detected by chemiluminescence. Data kindly provided by Dr. L. Jia, Ann Arbor, USA. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

APPL1 / DIP13A antibody

Human THP-1; WB Suggested Anti-APPL1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: THP-1 cell lysate; APPL1 antibody - middle region (ARP54834_P050) in Human THP-1 cells using Western Blot Rabbit Aff - Purified Hu, Ms WB
50 µg / €350.00
  AVIVA Systems Biology

APPL1 / DIP13A antibody

APPL1 / DIP13A Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €290.00
  Boster Immunoleader

CRISPR

Catalog No. Species Donor Vector  

APPL1 - human gene knockout kit via CRISPR

APPL1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

APPL1 - human gene knockout kit via CRISPR

APPL1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

APPL1 - human gene knockout kit via CRISPR

APPL1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Appl1 - mouse gene knockout kit via CRISPR

Appl1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Appl1 - mouse gene knockout kit via CRISPR

Appl1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Appl1 - mouse gene knockout kit via CRISPR

Appl1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Appl1 - mouse gene knockout kit via CRISPR

Appl1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

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