APRT antibody

Principal name

APRT antibody

Alternative names for APRT antibody

Adenine phosphoribosyltransferase

SwissProt ID

P07741 (Human)

Gene ID

353 (APRT)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Rt (Rat), Ms (Mouse)

Available hosts

Mouse, Rabbit

Available applications

ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Flow Cytometry (F)

Background of APRT antibody

Adenine phosphoribosyltransferase (APRT) belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene.Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene.

General readings

Baranowska-Bosiacka I., et al. (2009) Toxicology. 259(1-2):77-83.
Liang L., et al. (2007) Cancer Res. 67(5):1910-7.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

APRT antibody

  Rabbit polyclonal antibody to APRT (adenine phosphoribosyltransferase)  
APRT antibody detects APRT protein by Western blot analysis. A. 30 ug 293T whole cell lysate/extract. B. 30 ug A431 whole cell lysate/extract. 15 % SDS-PAGE. APRT antibody (TA307909) dilution: 1:1000 Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

APRT (N-term) antibody

  Rabbit polyclonal APRT Antibody (N-term)  
APRT Antibody (N-term) (Cat. #TA324753) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the APRT antibody detected the APRT protein (arrow). Rabbit Hu F, ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

APRT (C-term) antibody

  Rabbit polyclonal APRT Antibody (C-term)  
APRT Antibody (C-term) (Cat. #TA324754) western blot analysis in MCF-7 cell line and rat kidney tissue lysates (35ug/lane).This demonstrates the APRT antibody detected the APRT protein (arrow). Rabbit Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

APRT antibody

  Rabbit anti-APRT Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using APRT antibody. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

APRT antibody

  Rabbit Polyclonal Anti-APRT Antibody - N-terminal region  
WB Suggested Anti-APRT Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: 293T cell lysateAPRT is strongly supported by BioGPS gene expression data to be expressed in Human HEK293T cells Rabbit IgG Purified Can, Hu, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

CRISPR

Catalog No. Species Donor Vector  

APRT - human gene knockout kit via CRISPR

APRT - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

APRT - human gene knockout kit via CRISPR

APRT - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

APRT - human gene knockout kit via CRISPR

APRT - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

APRT - human gene knockout kit via CRISPR

APRT - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

APRT (transcript variant 1)

  Recombinant protein of human adenine phosphoribosyltransferase (APRT), transcript variant 1  
APRT Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

APRT (1-180)

Recombinant human APRT, 1-180 aa: 15% SDS-PAGE (3 µg) Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €670.00
  Acris Antibodies GmbH

APRT (1-180)

Recombinant human APRT, 1-180 aa: 15% SDS-PAGE (3 µg) Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €250.00
  Acris Antibodies GmbH

Lysates

Catalog No.    

APRT overexpression lysate

APRT overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

APRT overexpression lysate

APRT overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

APRT overexpression lysate

APRT overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, APRT (mGFP-tagged) - Human adenine phosphoribosyltransferase (APRT), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, APRT (mGFP-tagged) - Human adenine phosphoribosyltransferase (APRT), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, APRT (Myc-DDK tagged) - Human adenine phosphoribosyltransferase (APRT), transcript variant 2 , 200 uL, >10^7 TU/mL

Lenti ORF particles, APRT (Myc-DDK tagged) - Human adenine phosphoribosyltransferase (APRT), transcript variant 2 , 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

17 Item(s)

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