Aquaporin-10 / AQP10 antibody

Principal name

Aquaporin-10 / AQP10 antibody

Alternative names for Aquaporin-10 / AQP10 antibody

AQP-10, Small intestine aquaporin

SwissProt ID

Q96PS8 (Human)

Gene ID

89872 (AQP10)

Available reactivities

Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Rb (Rabbit), Ye (Yeast)

Available hosts

Rabbit

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)

Background of Aquaporin-10 / AQP10 antibody

Water channel proteins, known as aquaporins, are transmembrane proteins that mediate osmotic water permeability. The discovery of aquaporin (AQP) has made a great impact on life sciences. AQPs are a family of homologous water channels widely distributed in plants, unicellular organisms, invertebrates, and vertebrates. Aquaporins are selective water/glycerol channels involved in the maintenance of volume homeostasis and ionic/osmotic balance. These water channels also enhance cellular tolerance against rapid freezing, which suggest that they might have some ecological relevance also. Early work of Nobel Prize winner, Peter Agre and his group first clone a 269 amino acid protein from RBC and kidney libraries that has a 6 TMD (1). The amino terminal end of the protein was in the membrane and has 2 repeats that are at 180oto each other and are responsible for water selectivity. Now there are at least 12 isoforms of Aquaporins are cloned. In brain, three main AQPs AQP1, AQP4 and AQP9 are expressed and are implicated in numerous physiological functions. Aquaporins are characterized by presence of highly conserved asparagine proline alanine (NPA) boxes that are important for the formation of a water-permeating pore. AQP1 has a role in cerebrospinal fluid formation, whereas AQP4 is involved in water homeostasis and extracellular osmotic pressure in brain parenchyma. AQP4 seems also to have an important function in oedema formation after brain trauma or brain ischemia. AQP9 is implicated in brain energy metabolism (2). AQP1 and 4 are also implicated in water balance in retina and other eye components. Studies also suggest that AQP9 may be responsible for tumor angiogenesis and in the development of effusions or edema fluid and led to high vascular permeability and interstitial fluid pressure in tumors of the brain, colon, breast and pancreas
The expression of AQP10 is found in multiple rat tissues, including kidney, liver, testes and brain. AQP10 has a unique distribution in brain, appearing in Purkinje cell dendrites, hippocampal neurons of CA1 and CA2, and cerebral cortical neurons. Immunofluorescent staining of Purkinje cells indicates that AQP10 is intracellular. Unlike other aquaporins, Xenopus oocytes expressing AQP11 in the plasma membrane failed to transport water, glycerol, urea, or ions. AQP10 is functionally distinct from other proteins of the aquaporin superfamily and could represent a new aquaporin subfamily. The aquaporin 10 and 12 are localized intracellularly and have positively charged amino acid clusters at the C-terminal end identical to di-lysine (KKXX) motif for an endoplasmic retention signal. Disruption of Aquaporin 11 led to fatal polycystic kidneys (4). Further studies are necessary to elucidate the role of AQP11 in the brain (3).

General readings

Li,H., (2005) Biol. Cell 97 (11), 823-829

12 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Aquaporin-10 / AQP10 antibody

Gel: 8%SDS-PAGE,Lysate: 40 µg,,Primary antibody: AQP10 Antibody at dilution 1/200 dilution,Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,Exposure time: 10 minutes Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Aquaporin-10 / AQP10 antibody

WB Suggested Anti-AQP10 Antibody Titration: 0.2-1 ug/ml; Positive Control: Jurkat cell lysate Rabbit IgG Purified Bov, Hu, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Aquaporin-10 / AQP10 antibody

WB Suggested Anti-AQP10 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Human kidney Rabbit IgG Purified Hu, Rt, Ye WB
50 µg / €325.00
  OriGene Technologies, Inc.

Aquaporin-10 / AQP10 control peptide

Aquaporin-10 / AQP10
0.25 mg / €250.00
  Acris Antibodies GmbH

Aquaporin-10 / AQP10 (C-term amidated) antibody

Aquaporin-10 / AQP10 Rabbit Aff - Purified Ms, Rt E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

Aquaporin-10 / AQP10 antibody

Human Jurkat; WB Suggested Anti-AQP10 Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysate; AQP10 antibody - C-terminal region (ARP50244_P050) in Human Jurkat cells using Western Blot Rabbit Aff - Purified Can, Hu WB
50 µg / €350.00
  AVIVA Systems Biology

Aquaporin-10 / AQP10 antibody

Human kidney; WB Suggested Anti-AQP10 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human kidney; AQP10 antibody - middle region (ARP50245_P050) in Human kidney cells using Western Blot Rabbit Aff - Purified Can, Hu WB
50 µg / €350.00
  AVIVA Systems Biology

Aquaporin-10 / AQP10 antibody

Aquaporin-10 / AQP10 Rabbit IgG Aff - Purified Hu WB
0.1 mg / €290.00
  Boster Immunoleader

Aquaporin-10 / AQP10 antibody

Aquaporin-10 / AQP10 Rabbit Hu P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

Aquaporin-10 / AQP10 antibody

Western Blot: AQP10 Antibody [NBP1-62621] - This Anti-AQP10 antibody was used in Western Blot of Fetal Kidney   tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Can, Hu WB
50 µg / €440.00
  Novus Biologicals Inc.

Aquaporin-10 / AQP10 antibody

Western Blot: AQP10 Antibody [NBP1-69625] - This Anti-AQP10 antibody was used in Western Blot of Jurkat tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu WB
50 µg / €440.00
  Novus Biologicals Inc.

Lysates

Catalog No.    

AQP10 overexpression lysate

AQP10 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

12 Item(s)

per page
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