ARAF antibody

Principal name

ARAF antibody

Alternative names for ARAF antibody

A-raf-1, ARAF1, PKS, PKS2, Proto-oncogene Pks, A-Raf proto-oncogene serine/threonine-protein kinase

SwissProt ID

O19004 (Pig), P04627 (Mouse), P10398 (Human), P14056 (Rat)

Gene ID

369 (ARAF)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Por (Porcine), Sh (Sheep), Ze (Zebrafish), Eq (Equine)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of ARAF antibody

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.

General readings

Razzaque,M.A., (2007) Nat. Genet. 39 (8), 1013-1017

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ARAF antibody

Sample (30 ug of whole cell lysate). A: Hela. 7.5% SDS PAGE. TA308536 diluted at 1:1000. Rabbit IgG Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ARAF antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-ARAF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803663) Mouse IgG2a OTI2G9 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ARAF antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between PKM2 and ARAF. HeLa cells were stained with anti-PKM2 rabbit purified polyclonal 1:1200 and anti-ARAF mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG2a 3E2 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ARAF antibody

Western blot analysis of extracts from Cos7 and A549 cells, using A-RAF antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ARAF antibody

WB Suggested Anti-ARAF Antibody Titration: 0.2-1 ug/ml; Positive Control: Human brain Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rt, Sh WB
50 µg / €325.00
  OriGene Technologies, Inc.

ARAF antibody

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-ARAF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803658) Mouse IgG1 OTI2F8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ARAF antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARAF (RC200737, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARAF. Mouse IgG1 OTI2G4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ARAF antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARAF (RC200737, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARAF. Mouse IgG1 OTI2F5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ARAF antibody

Immunohistochemistry (IHC) analyzes of A-RAF antibody (Cat.-No.: AP06495PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

ARAF antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. Rabbit Ig Purified Hu P
0.4 ml / €370.00
  Acris Antibodies GmbH

ARAF antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between ARAF and BRAF. HeLa cells were stained with anti-ARAF rabbit purified polyclonal 1:1200 and anti-BRAF mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

ARAF antibody

ARAF monoclonal antibody (M01), clone 6H6 Western Blot analysis of ARAF expression in HeLa ( Cat # L013V1 ). Mouse IgG2a 6H6 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ARAF antibody

ARAF monoclonal antibody (M02), clone 5H8 Western Blot analysis of ARAF expression in HeLa ( Cat # L013V1 ). Mouse IgG2a 5H8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ARAF antibody

ARAF monoclonal antibody (M03), clone 4H7 Western Blot analysis of ARAF expression in HeLa ( Cat # L013V1 ). Mouse IgG2a 4H7 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ARAF antibody

ARAF monoclonal antibody (M04), clone 3G2 Western Blot analysis of ARAF expression in HeLa ( Cat # L013V1 ). Mouse IgG2a 3G2 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ARAF antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with ARAF polyclonal antibody ( Cat # PAB3478 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma. Rabbit Hu E, P
0.1 mg / €390.00
  Abnova Taiwan Corp.

ARAF pTyr302 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using A-RAF (Phospho-Tyr302) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €205.00
  Assay Biotech

ARAF pTyr302 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using A-RAF (Phospho-Tyr302) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €310.00
  Assay Biotech

ARAF antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using A-RAF (Ab-301/302) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €205.00
  Assay Biotech

ARAF antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using A-RAF (Ab-301/302) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €310.00
  Assay Biotech

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