ARF1 antibody

Principal name

ARF1 antibody

Alternative names for ARF1 antibody

ADP-ribosylation factor 1

SwissProt ID

P36397 (Arath), P51821 (Chlre), P84077 (Human), P84078 (Mouse), P84079 (Rat), P84080 (Bovin)

Gene ID

375 (ARF1), 64310 (Arf1), 819330 (ARF1A1C)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Por (Porcine), Ze (Zebrafish), African clawed frog, Pl (Plant), Fi (Fish), Xen (Xenopus), GP (Guinea Pig), Mky (Monkey), Rb (Rabbit), Sh (Sheep), Hst (Hamster)

Available hosts

Mouse, Rabbit, Sheep, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Immunoprecipitation (IP), Dot blot (Dot)

Background of ARF1 antibody

Ras superfamily of small GTPases regulates a wide variety of cellular processes including intracellular trafficking of proteins. The Ras proteins acts as molecular switches by cycling between inactive GDP-bound and active GTP-bound state. Guanine exchange factors (GEFs) Among the various Ras proteins, the ADP-ribosylation factor proteins (ARFs) are well characterized regulators of vesicle formation in intracellular membrane traffic while Rho proteins regulate the actin cytoskeleton (1). The Arf family members have unique structural device, called the ‘interswitch toggle’, that implements communication between the nucleotide-binding site and a unique N-terminal amphipathic helix that mediates the interaction of Arf proteins with membranes, these structural motifs distinguish them from other small GTPases (2). When GDP is bound to ARF proteins the amphipathic helix is positioned in a hydrophobic pocket, whereas in the GTP-bound forms it is displaced by the interswitch toggle and stabilizes interaction of the protein with membrane lipid bilayer. Thus, the GTP-GDP cycle is coupled to membrane-cytosol cycle and thereby ARF proteins are able to regulate vesicular traffic and organelle structure formation (2).
The mammalian ARF family comprise of 5 members (ARF1-6), there is no ARF2. ARF1 and ARF6 are most abundantly expressed and located in distinct cellular locations at Golgi complex and early-endosome-plasma membrane interface and are most distantly related with only 67% sequence identity. ARF1 is mostly known for its role in vesicle budding and Golgi regulation through the recruitment of coat proteins and in the regulation of lipid-modifying enzymes and actin organizing components (3). The crystal structure of ARF-BD (ARF binding domain) comprise of a PH domain adjacent to a C-terminal helix and ARF interacts with these two regions via its “interswitch toggle” (4). ARF1 protein has 188 amino acids and is expressed in at least 3 variant forms, ARF1 antibodies will label all variants equally. The deactivation of ARF1 induces redistribution of entire Golgi complex to ER, the GTP bound form triggers the association with coat protein with the Golgi membranes. Purified ARF proteins do not have GTPase activity suggesting that GTP hydrolysis is dependent on GTPase activating protein (GAP) a 49kDa protein (4). The GTP bound ARF1 and 3 proteins also interact with several interacting protein (ARFGAPs) including protein interacting with C kinase 1 (PICK1) to mediate their cellular processes.

General readings

This product has been used in:Cavenagh MM et al. Intracellular distribution of Arf proteins in mammalian cells. Arf6 is uniquely localized to the plasma membrane. J Biol Chem 271:21767-74 (1996). PubMed PMID: 8702973
General / background references:Happe S & Weidman P Cell-free transport to distinct Golgi cisternae is compartment specific and ARF independent. J Cell Biol 140:511-23 (1998). PubMed PMID: 9456313
Faundez V et al. ADP ribosylation factor 1 is required for synaptic vesicle budding in PC12 cells. J Cell Biol 138:505-15 (1997). PubMed PMID: 9245782
Boman AL & Kahn RA Arf proteins: the membrane traffic police? Trends Biochem Sci 20:147-50 (1995). PubMed PMID: 7770914
Zeuzem S et al. Intravesicular acidification correlates with binding of ADP-ribosylation factor to microsomal membranes. Proc Natl Acad Sci U S A 89:6619-23 (1992). PubMed PMID: 1385876
Serafini T et al. ADP-ribosylation factor is a subunit of the coat of Golgi-derived COP-coated vesicles: a novel role for a GTP-binding protein. Cell 67:239-53 (1991). PubMed PMID: 1680566

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ARF1 antibody

ARF1 antibody detects ARF1 protein by Western blot analysis. A. 30 ug Neuro2A whole cell lysate/extract. B. 30 ug GL261 whole cell lysate/extract. C. 30 ug C8D30 whole cell lysate/extract. D. 30 ug NIH-3T3 whole cell lysate/extract. E. 30 ug BCL-1 whole c Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ARF1 antibody

Sample (50 ug of whole cell lysate). A: mouse brain. 12% SDS PAGE. TA308576 diluted at 1:10000. Rabbit IgG Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ARF1 antibody

Mouse brain; Researcher: Dr. Yuzhi Chen, University of Arkansas for Medical Science. Application: IHC. Species+tissue/cell type: Mouse brain stem cells. Primary antibody dilution: 1:500. Secondary antibody: Goat anti-rabbit Alexa-Fluor 594. Secondary antibody dilution: 1:1000; ARF1 antibody - middle region (ARP48184_P050) in Mouse brain cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Por, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

ARF1 antibody

Gel: 12%SDS-PAGE, Lysate: 40 µg, Lane 1-2: Human fetal brain tissue, Human kidney tissue, Primary antibody: ARF1 Antibody at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ARF1 antibody

Western blot analysis: The cell lysates (35 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ARF1 antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 AT1B3 Purified Hu E, F, ICC/IF, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

ARF1 antibody

Western blot analysis: The cell lysates (35 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ARF1 antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 AT1B3 Purified Hu E, F, ICC/IF, WB
50 µl / €230.00
  Acris Antibodies GmbH

ARF1 Control Peptide

ARF1
0.25 mg / €250.00
  Acris Antibodies GmbH

ARF1 antibody

ARF1 Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

ARF1 antibody

ARF1 Mouse Serum Hu E
50 µl / €290.00
  Abnova Taiwan Corp.

ARF1 antibody

ARF1 Mouse IgG2a 4G6 Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

ARF1 antibody

The ARF1/ARF2/ARF3/ARF4 polyclonal antibody ( Cat # PAB7171 ) ( 1 µg/ml ) staining of HepG2 cell lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat IgG Purified Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

ARF1 antibody

ARF1 Rabbit Serum Arabidopsis thaliana, Chlamydomonas reinhardtiia, Nicotiana tabacum, Pl WB
50 µl / €300.00
  AgriSera AB

ARF1 antibody

Human Fetal Muscle; WB Suggested Anti-ARF1 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Fetal Muscle; ARF1 antibody - middle region (ARP48183_P050) in Human Fetal Muscle cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

ARF1 antibody

Western Blot: ARF1 Antibody [NB100-55421] - NB100-55421 (1ug/ml) staining of HepG2 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

ARF1 antibody

Immunohistochemistry: ARF1 Antibody (EP442Y) [NB110-55468] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-ARF1 Rabbit IgG EP442Y Purified Hu, Ms, Rt F, P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

ARF1 antibody

Western Blot: ARF1 Antibody [NB110-85530] - Lane 1: MWM, Lane 2: Rat Brain, Lane 3: Mouse Brain, Lane 4: MDCK Mouse ARFS 3F1 Purified Bov, Can, Chk, GP, Hu, Mky, Ms, Por, Rb, Rt, Sh WB
50 µg / €380.00
  Novus Biologicals Inc.

ARF1 antibody

ARF1 Mouse IgG1 1D9 Ascites Bov, Can, Hu, Ms, Rt F, ICC/IF, IP, P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

ARF1 antibody

Western Blot: ARF1 Antibody [NBP1-31003] - Western blot analysis of ARF1 Antibody on: A:Hep G2(GTX27900) 12% SDS PAGE Diluted at 1:500 Rabbit IgG Hu Dot, P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

ARF1 antibody

(30 ug of whole cell lysate)  A: A431  B: H1299  12% SDS PAGE diluted at 1:1000 Rabbit IgG Hu ICC/IF, P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

ARF1/2/3/4 (C-term) antibody

TA303176 (1µg/ml) staining of HepG2 cell lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

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