ARF1 antibody

Principal name

ARF1 antibody

Alternative names for ARF1 antibody

ADP-ribosylation factor 1

SwissProt ID

P36397 (Arath), P51821 (Chlre), P84077 (Human), P84078 (Mouse), P84079 (Rat), P84080 (Bovin)

Gene ID

375 (ARF1), 819330 (ARF1A1C), 64310 (Arf1), 11840

Available reactivities

Hu (Human), Pl (Plant), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Por (Porcine), Ze (Zebrafish), African clawed frog

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of ARF1 antibody

Ras superfamily of small GTPases regulates a wide variety of cellular processes including intracellular trafficking of proteins. The Ras proteins acts as molecular switches by cycling between inactive GDP-bound and active GTP-bound state. Guanine exchange factors (GEFs) Among the various Ras proteins, the ADP-ribosylation factor proteins (ARFs) are well characterized regulators of vesicle formation in intracellular membrane traffic while Rho proteins regulate the actin cytoskeleton (1). The Arf family members have unique structural device, called the ‘interswitch toggle’, that implements communication between the nucleotide-binding site and a unique N-terminal amphipathic helix that mediates the interaction of Arf proteins with membranes, these structural motifs distinguish them from other small GTPases (2). When GDP is bound to ARF proteins the amphipathic helix is positioned in a hydrophobic pocket, whereas in the GTP-bound forms it is displaced by the interswitch toggle and stabilizes interaction of the protein with membrane lipid bilayer. Thus, the GTP-GDP cycle is coupled to membrane-cytosol cycle and thereby ARF proteins are able to regulate vesicular traffic and organelle structure formation (2).
The mammalian ARF family comprise of 5 members (ARF1-6), there is no ARF2. ARF1 and ARF6 are most abundantly expressed and located in distinct cellular locations at Golgi complex and early-endosome-plasma membrane interface and are most distantly related with only 67% sequence identity. ARF1 is mostly known for its role in vesicle budding and Golgi regulation through the recruitment of coat proteins and in the regulation of lipid-modifying enzymes and actin organizing components (3). The crystal structure of ARF-BD (ARF binding domain) comprise of a PH domain adjacent to a C-terminal helix and ARF interacts with these two regions via its “interswitch toggle” (4). ARF1 protein has 188 amino acids and is expressed in at least 3 variant forms, ARF1 antibodies will label all variants equally. The deactivation of ARF1 induces redistribution of entire Golgi complex to ER, the GTP bound form triggers the association with coat protein with the Golgi membranes. Purified ARF proteins do not have GTPase activity suggesting that GTP hydrolysis is dependent on GTPase activating protein (GAP) a 49kDa protein (4). The GTP bound ARF1 and 3 proteins also interact with several interacting protein (ARFGAPs) including protein interacting with C kinase 1 (PICK1) to mediate their cellular processes.

General readings

1. Hall A (2005) Rho GTPases and the control of cell behavior. Biochem Soc Trans 33: 891–895
2. Antonny B, Beraud-Dufour S, Chardin P, Chabre M (1997) Biochemistry 36: 4675–4684
3. D'Souza-Schorey C, Chavrier P (2006) ARF proteins: roles in membrane traffic and beyond. Nat Rev Mol Cell Biol 7: 347–358
4. Julie Ménétrey, Mylène Perderiset,Jérome Cicolari, Thierry Dubois, Nadia Elkhatib, Fatima El Khadali, Michel Franco, Philippe Chavrier, and Anne Houdusse. EMBO J .26(7); Apr 4, 2007
5. Makler V, Cukierman E, Rotman M, Admon A, Cassel D. ADP-ribosylation factor-directed GTPase-activating protein. Purification and partial characterization. J Biol Chem. 1995 Mar
10;270(10):5232-7. Links

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ARF1 antibody

  Rabbit Polyclonal antibody to ARF1 (ADP-ribosylation factor 1)  
ARF1 antibody detects ARF1 protein by Western blot analysis. A. 30 ug Neuro2A whole cell lysate/extract. B. 30 ug GL261 whole cell lysate/extract. C. 30 ug C8D30 whole cell lysate/extract. D. 30 ug NIH-3T3 whole cell lysate/extract. E. 30 ug BCL-1 whole c Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ARF1 antibody

  Rabbit Polyclonal antibody to ARF1 (ADP-ribosylation factor 1)  
Sample (50 ug of whole cell lysate). A: mouse brain. 12% SDS PAGE. TA308576 diluted at 1:10000. Rabbit IgG Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ARF1 antibody

  Anti-ARF1 Rabbit Polyclonal Antibody  
Gel: 12%SDS-PAGE, Lysate: 40 µg, Lane 1-2: Human fetal brain tissue, Human kidney tissue, Primary antibody: ARF1 Antibody at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ARF1 antibody

The cell lysates (35 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ARF1 antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 AT1B3 Purified Hu E, F, ICC/IF, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

ARF1 antibody

The cell lysates (35 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ARF1 antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 AT1B3 Purified Hu E, F, ICC/IF, WB
50 µl / €230.00
  Acris Antibodies GmbH

ARF1/2/3/4 (C-term) antibody

  Goat Polyclonal Antibody against ARF1  
TA303176 (1µg/ml) staining of HepG2 cell lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CRISPR

Catalog No. Species Donor Vector  

ARF1 - human gene knockout kit via CRISPR

ARF1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

ARF1 - human gene knockout kit via CRISPR

ARF1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

ARF1 - human gene knockout kit via CRISPR

ARF1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

ARF1 - human gene knockout kit via CRISPR

ARF1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Arf1 - mouse gene knockout kit via CRISPR

Arf1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Arf1 - mouse gene knockout kit via CRISPR

Arf1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Arf1 - mouse gene knockout kit via CRISPR

Arf1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Arf1 - mouse gene knockout kit via CRISPR

Arf1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ARF1 (transcript variant 1)

  Recombinant protein of human ADP-ribosylation factor 1 (ARF1), transcript variant 1  
ARF1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

ARF1 (transcript variant 4)

  Recombinant protein of human ADP-ribosylation factor 1 (ARF1), transcript variant 4  
ARF1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

ARF1 (transcript variant 3)

  Recombinant protein of human ADP-ribosylation factor 1 (ARF1), transcript variant 3  
ARF1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

ARF1 (1-181, His-tag)

Recombinant human ARF1, 1-181 aa, His-tagged Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €750.00
  Acris Antibodies GmbH

ARF1 (1-181, His-tag)

Recombinant human ARF1, 1-181 aa, His-tagged Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

Lysates (Knockout)

Catalog No.    

ARF1-knockout Cell lysate

ARF1-knockout Cell lysate  
0.1 mg / €495.00
  OriGene Technologies, Inc.

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