ARF4 antibody

Principal name

ARF4 antibody

Alternative names for ARF4 antibody

ARF2, ADP-ribosylation factor 4

SwissProt ID

P18085 (Human), P61750 (Mouse), P61751 (Rat), Q3SZF2 (Bovin)

Gene ID

378 (ARF4), 79120 (Arf4), 11843

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Por (Porcine), Mky (Monkey)

Available hosts

Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Frozen Sections (C), Immunoprecipitation (IP), Paraffin Sections (P)

Background of ARF4 antibody

The Ras super-family of small GTPases regulates a wide variety of cellular processes including intracellular trafficking of proteins. Within this family the ADP-ribosylation factors (Arfs) are six proteins (ARF1-6) which share numerous biochemical activities and have very similar specific activities and they all regulate membrane traffic. The Ras proteins acts as molecular switches by cycling between inactive GDP-bound and active GTP-bound state. Guanine exchange factors (GEFs) Among the various Ras proteins, the ADP-ribosylation factor proteins (ARFs) are well characterized regulators of vesicle formation in intracellular membrane traffic while Rho proteins regulate the actin cytoskeleton (1). The Arf family members have unique structural device, called the ‘interswitch toggle’, that implements communication between the nucleotide-binding site and a unique N-terminal amphipathic helix that mediates the interaction of Arf proteins with membranes, these structural motifs distinguish them from other small GTPases (2). When GDP is bound to ARF proteins the amphipathic helix is positioned in a hydrophobic pocket, whereas in the GTP-bound forms it is displaced by the interswitch toggle and stabilizes interaction of the protein with membrane lipid bilayer. Thus, the GTP-GDP cycle is coupled to membrane-cytosol cycle and thereby ARF proteins are able to regulate vesicular traffic and organelle structure formation (2).
The mammalian ARF family comprise of 5 members (ARF1-6), there is no ARF2. ARF1 and ARF6 are most abundantly expressed and located in distinct cellular loctions at golgi coples and early-endosome-plasma membrane interface and are most distantly related with only 67% sequence identity. ARF1 is mostly known for its role in vesicle budding and golgi regulation through the recruitment of coat proteins and in the regulation of lipid-modifying enzymes and actin organizing components (3). The crystal structure of ARF-BD (ARF binding domain) comprise of a PH domain adjacent to a C-terminal helix and ARF interacts with these two regions via its “interswitch toggle” (4). ARF1 protein has 188 amino acids and is expressed in at least 3 variant forms, ARF1 antibodies will label all variants equally. The deactivation of ARF1 induces redistribution of entire Golgi comples to ER, the GTP bound form triggers the association with coat protein with the Golgi membranes. Purified ARF proteins do not have GTPase activity suggesting that GTP hydrolysis is dependent on GTPase activating protein (GAP) a 49kDa protein (4). The GTP bound ARF1 and 3 proteins also interact with several interacting protein (ARFGAPs) including protein interacting with C kinase 1 (PICK1) to mediate their cellular processes. The distribution of ARFs with epitope tagged ARFs revealed ARF1-3 displayed a perinuclear localization while ARF4-6 appear to be widely distributed throughout the cytoplasm.

General readings

Mazelova J., et al. (2009) EMBO J. 28(3):183-92.
Woo IS., et al. (2009) Cancer Lett. 276(1):53-60.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ARF4 (137-150) antibody

(0.5ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Hu, Ms, Por, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ARF4 antibody

Western blot analysis of extracts from LOVO cells, K562 cells and HT-29 cells, using ARF4 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ARF4 antibody

Predicted cell location: Cytoplasm. Positive control: Human breast cancer tissue. Recommended dilution: 1/15-50  The image on the left is immunohistochemistry of paraffin-embedded human breast cancer tissue using ARF4 antibody at dilution 1/20, on the right is treated with the fusion protein. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

ARF4 antibody

WB Suggested Anti-Arf4 Antibody; Titration: 1.0 ug/ml; Positive Control: Mouse Brain Rabbit IgG Purified Bov, Hu, Ms, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

ARF4 Control Peptide

ARF4
0.25 mg / €250.00
  Acris Antibodies GmbH

ARF4 antibody

ARF4 Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

ARF4 antibody

Western blot analysis of extracts from LOVO/K562/HT-29 cells, using ARF4 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

ARF4 antibody

Western blot analysis of extracts from LOVO/K562/HT-29 cells, using ARF4 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

ARF4 (N-term) antibody

Mouse Brain; WB Suggested Anti-Arf4 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Mouse Brain; Arf4 antibody - N-terminal region (ARP56032_P050) in Mouse Brain cells using Western Blot Rabbit Aff - Purified Hu, Ms WB
50 µg / €350.00
  AVIVA Systems Biology

ARF4 antibody

Immunohistochemical analysis of ARF4 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Mky, Ms, Rt IP, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

ARF4 antibody

Immunohistochemical analysis of ARF4 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Mky, Ms, Rt IP, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

ARF4 antibody

Immunohistochemical analysis of ARF4 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Mky, Ms, Rt IP, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

ARF4 antibody

ARF4 Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

ARF4 antibody

ARF4 Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
50 µg / €290.00
  Novus Biologicals Inc.

ARF4 antibody

ARF4 Rabbit Aff - Purified Hu, Ms WB
50 µg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ARF4

ARF4 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

ARF4 (1-180, His-tag)

ARF4 Human Purified > 95 % E. coli
0.5 mg / €1,000.00
  Acris Antibodies GmbH

ARF4 (1-180, His-tag)

ARF4 Human Purified > 95 % E. coli
0.1 mg / €370.00
  Acris Antibodies GmbH

ARF4

ARF4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

ARF4

ARF4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

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