Arfaptin-1 antibody

Principal name

Arfaptin-1 antibody

Alternative names for Arfaptin-1 antibody

ARFIP1, ADP-ribosylation factor-interacting protein 1

SwissProt ID

P53367 (Human), Q9JHU5 (Rat)

Gene ID

27236 (ARFIP1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of Arfaptin-1 antibody

ADP-ribosylation factors, or ARFs, enhance the ADP ribosyltransferase activity of cholera toxin and are implicated in vesicle transport between endoplasmic reticulum and the Golgi complex. Arfaptin 1 is recruited from the cytosol to Golgi membranes by ARFs in a guanosine 5'-O-(3-thiotriphosphate)-dependent and brefeldin A-sensitive manner but is not a constituent of coatomer. Arfaptin 1 binds to nonmyristoylated GTP-bound ARF3, but not to GDP-bound ARF3, and also to ARF1, another class I ARF. It binds with lower affinity to ARF5, a class II ARF, and with very little affinity to ARF6, a class III ARF. POR1 (also designated arfaptin 2) was first isolated as a Rac 1 binding protein necessary for Rac mediated Actin polymerization and the subsequent formation of membrane ruffles and lamellipodia. POR1 has also been shown to interact with the ADP ribosylation factor ARF6, a GTPase that associates with the plasma membrane and intracellular endosome vesicles, in a GTP dependent manner. The association of POR1 with ARF6 stimulates induction of Actin polymerization. POR1 appears to play a regulatory role through multiple signaling pathways in the reorganization of the cytoskeletal structure.

General readings

Kanoh H., et al. (1997) J. Biol. Chem. 272:5421-5429Williger B.-T., et al. (1999) FEBS Lett. 454:85-89

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Arfaptin-1 antibody

Western blot analysis of extracts from Jurkat cells, using ARFIP1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Arfaptin-1 antibody

WB Suggested Anti-ARFIP1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: 293T cell lysateARFIP1 is supported by BioGPS gene expression data to be expressed in HEK293T Rabbit IgG Purified Bov, Can, Eq, Hu, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Arfaptin-1 antibody

Immunohistochemistry analysis of Arfaptin-1 antibody Cat.-No AP01383PU-N in paraffin-embedded human thymus gland tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Arfaptin-1 (Center) antibody

ARFIP1 Antibody (Center) (Cat. #AP12992c)immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ARFIP1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Arfaptin-1 antibody

Western Blot analysis of ARFIP1 expression in transfected 293T cell line (H00027236-T01) by ARFIP1 MaxPab polyclonal antibody.

Lane 1: ARFIP1 transfected lysate(41.03 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

Arfaptin-1 antibody

Arfaptin-1 Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Arfaptin-1 antibody

Immunoperoxidase of monoclonal antibody to ARFIP1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml] Mouse IgG2a 1F10 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Arfaptin-1 antibody

Immunofluorescence analysis of A549 cells, using ARFIP1 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
50 µg / €205.00
  Assay Biotech

Arfaptin-1 antibody

Immunofluorescence analysis of A549 cells, using ARFIP1 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €310.00
  Assay Biotech

Arfaptin-1 (C-term) antibody

Arfaptin-1 Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

Arfaptin-1 antibody

Immunohistochemical analysis of ARFIP1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Ms, Rt ICC/IF, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

Arfaptin-1 antibody

Immunohistochemical analysis of ARFIP1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

Arfaptin-1 antibody

Immunohistochemical analysis of ARFIP1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Ms, Rt ICC/IF, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

Arfaptin-1 antibody

Immunohistochemistry: Arfaptin 1 Antibody [NBP1-00718] - Immunohistochemistry (IHC) analyzes of Arfaptin 1 (K314) antibody in paraffin-embedded human thymus gland tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

Arfaptin-1 antibody

Arfaptin-1 Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

Arfaptin-1 antibody

Arfaptin-1 Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
50 µg / €290.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Arfaptin-1 (full length, N-term HIS tag, transcript variant 2)

Arfaptin-1 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

Arfaptin-1 (1-341, His-tag)

Arfaptin-1 Human Purified > 90 % by SDS - PAGE E. coli
0.25 mg / €730.00
  Acris Antibodies GmbH

Arfaptin-1 (1-341, His-tag)

Arfaptin-1 Human Purified > 90 % by SDS - PAGE E. coli
50 µg / €300.00
  Acris Antibodies GmbH

Arfaptin-1 (1-373, His-tag)

Arfaptin-1 Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €730.00
  Acris Antibodies GmbH

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