Arginosuccinase antibody

Principal name

Arginosuccinase antibody

Alternative names for Arginosuccinase antibody

Argininosuccinate lyase, ASAL, ASL

SwissProt ID

P04424 (Human), P20673 (Rat), Q3SZJ0 (Bovin), Q91YI0 (Mouse)

Gene ID

435 (ASL), 109900 (Asl), 59085

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Chk (Chicken), Rt (Rat), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Gt (Goat), Por (Porcine), Rb (Rabbit), Ye (Yeast)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P)

Background of Arginosuccinase antibody

ASL is a member of the lyase 1 family. The protein forms a cytosolic homotetramer and primarily catalyzes the reversible hydrolytic cleavage of argininosuccinate into arginine and fumarate, an essential step in the liver in detoxifying ammonia via the urea cycle. Mutations in its gene result in the autosomal recessive disorder argininosuccinic aciduria, or argininosuccinic acid lyase deficiency.This gene encodes a member of the lyase 1 family. The encoded protein forms a cytosolic homotetramer and primarily catalyzes the reversible hydrolytic cleavage of argininosuccinate into arginine and fumarate, an essential step in the liver in detoxifying ammonia via the urea cycle. Mutations in this gene result in the autosomal recessive disorder argininosuccinic aciduria, or argininosuccinic acid lyase deficiency. A nontranscribed pseudogene is also located on the long arm of chromosome 22. Alternatively spliced transcript variants encoding different isoforms have been described.

General readings

Barbosa P,, et al. (2008). J Biol Chem. 266(8):5286-90Zhao S., et al (2010) Science 327:1000-1004

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Arginosuccinase antibody

ASL antibody detects ASL protein by Western blot analysis. A. 30 ug Neuro2A whole lysate/extract. B. 30 ug GL261 whole cell lysate/extract. C. 30 ug C8D30 whole cell lysate/extract. D. 30 ug NIH-3T3 whole cell lysate/extract. E. 30 ug BCL-1 whole cell lys Rabbit IgG Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Arginosuccinase antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-ASL mouse monoclonal antibody. (TA802245) Dilution: 1:150 Mouse IgG2a OTI3D4 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Arginosuccinase antibody

Immunofluorescence of monoclonal antibody to ASL on HeLa cell. [antibody concentration 5 ug/ml] Mouse IgG1 4C5-1F2 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Arginosuccinase antibody

Sample (30 ug of whole cell lysate). A: MOLT4 . 10% SDS PAGE. TA308132 diluted at 1:1000 Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Arginosuccinase antibody

WB Suggested Anti-ASL Antibody Titration: 0.2-1 ug/ml; Positive Control: Transfected 293T Rabbit IgG Purified Bov, Can, Eq, GP, Gt, Hu, Ms, Por, Rb, Rt, Ye, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Arginosuccinase antibody

WB Suggested Anti-ASL Antibody Titration: 5.0 ug/ml; Positive Control: Human Liver Rabbit IgG Purified Bov, Can, Eq, GP, Gt, Hu, Ms, Por, Rb, Rt, Ye, Ze WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Arginosuccinase antibody

The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using (ASL Antibody) at dilution 1/35, on the right is treated with fusion protein. (Original magnification: ×200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Arginosuccinase antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ASL (RC201568, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ASL. Mouse IgG1 OTI14C7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Arginosuccinase antibody

Western Blot analysis of ASL expression in transfected 293T cell line (H00000435-T01) by ASL MaxPab polyclonal antibody.

Lane 1: ASL transfected lysate(51.04 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Arginosuccinase antibody

ASL MaxPab rabbit polyclonal antibody. Western Blot analysis of ASL expression in mouse kidney. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Arginosuccinase antibody

Transfected 293T; WB Suggested Anti-ASL Antibody Titration: 0.2-1 ug/ml. Positive Control: Transfected 293T; ASL antibody - N-terminal region (ARP41666_P050) in Transfected 293T cells using Western Blot Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

Arginosuccinase antibody

Human; . Lanes: Lane1: 10 ug COS-7 cell lysate. Primary Antibody Dilution: 1:1000. Secondary Antibody: Anti-rabbit HRP. Secondary Antibody Dilution: 1:2000. Gene Name: ASL. Submitted by: Shawn Elms. Georgia Health Science University.; ASL antibody - middle region (ARP41667_T100) in Human cells using Western Blot Rabbit Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt, Ze WB
0.1 mg / €310.00
  AVIVA Systems Biology

CRISPR

Catalog No. Species Donor Vector  

Asl - mouse gene knockout kit via CRISPR

  Asl - mouse gene knockout kit via CRISPR  
Asl - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Asl - mouse gene knockout kit via CRISPR

  Asl - mouse gene knockout kit via CRISPR  
Asl - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Asl - mouse gene knockout kit via CRISPR

  Asl - mouse gene knockout kit via CRISPR  
Asl - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Asl - mouse gene knockout kit via CRISPR

  Asl - mouse gene knockout kit via CRISPR  
Asl - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Arginosuccinase (transcript variant 1)

Arginosuccinase Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Arginosuccinase (transcript variant 2)

Arginosuccinase Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Arginosuccinase (1-464, His-tag)

Arginosuccinase Human Purified > 95 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

Arginosuccinase (1-464, His-tag)

Arginosuccinase Human Purified > 95 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

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