Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E)
Background of ARHGAP10 / GRAF2 antibody
GTPase activator for the small GTPases RhoA and Cdc42 by converting them to an inactive GDP-bound state. Essential for PTKB2 regulation of cytoskeletal organization via Rho family GTPases. Inhibits PAK2 proteolytic fragment PAK-2p34 kinase activity and changes its localization from the nucleus to the perinuclear region. Stabilizes PAK-2p34 thereby increasing stimulation of cell death (By similarity).
ARHGAP10 antibody (Center) (Cat. #AP50232PU-N) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ARHGAP10 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
ARHGAP10 Antibody (Center) (Cat. #AP50232PU-N) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.