Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of ARHGAP13 / SRGAP1 antibody
SRGAP1/ARHGAP13 is a member of the srGAP family of proteins that contain a RhoGAP domain, an SH3 domain, and a Fes/CIP4 homology domain and interact with the intracellular domain of roundabout 1 (Robo1). Robo1 is the receptor for Slit signaling and is involved in neuronal cell migration and axon tract positioning. SRGAP1/ARHGAP13 functions to bind and decrease the level of active Cdc42 and RhoA small GTPases during Slit-Robo signaling. Alternate names for SRGAP1/ARHGAP13 include Rho GTPase-activating protein 13, and KIAA1304.
Immunoperoxidase of monoclonal antibody to SRGAP1 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml]
Western Blot: SRGAP1/ARHGAP13 Antibody [NB100-60649] - Detection of Human SRGAP1/ARHGAP13 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SRGAP1/ARHGAP13 antibody NB100-60649 5Aused for IP at 3 mcg/mg lysate. SRGAP1/ARHGAP13 was also immunoprecipitated by rabbit anti-SRGAP1/ARHGAP13 antibody NB100-60650, which recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 1 second."
WB and IP: SRGAP1 Antibody [NB100-60650] - SRGAP1/ARHGAP13 Antibody [NB100-60650] - Detection of Human SRGAP1/ARHGAP13 on HeLa whole cell lysate using NB100-60650. SRGAP1/ARHGAP13 was also IPed by NB100-60649.
Western Blot analysis of SRGAP1 expression in transfected 293T cell line (H00057522-T01) by SRGAP1 MaxPab polyclonal antibody.Lane 1: SRGAP1 transfected lysate(119.35 KDa).Lane 2: Non-transfected lysate.
> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.