ARHGAP2 / CHN1 antibody

Principal name

ARHGAP2 / CHN1 antibody

Alternative names for ARHGAP2 / CHN1 antibody

N-chimaerin, Alpha-chimerin, A-chimaerin, Rho GTPase-activating protein 2

SwissProt ID

P15882 (Human), P15882-2, P30337 (Rat), Q17QN0 (Bovin), Q91V57 (Mouse)

Gene ID

1123 (CHN1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Dros (Drosophila), Ze (Zebrafish), African clawed frog, Can (Canine), Mky (Monkey), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Ye (Yeast)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of ARHGAP2 / CHN1 antibody

Chimaerins are members of a Rho GTPase-activating proteins (GAPs) that comprises of beta1, alfa1 and alfa 2-chimaerins and the alternatively spliced variants from the beta and alfa-chimaerin genes. These proteins bind to diacylglycerol and phorbol esters like protein kinaseC. They all have a common single C1 domain which is capable of binding phorbol esters and other DAG-mimetics with low nonomolar affinity in the presence of acidic phospholipids. There are two types of chimaerins, alfa2 and beta2 Chimaerins both exhibit an N-terminal SH2 binding domain that interacts with phosphotyrosine containing proteins. The C-terminal chimaerin GTPase activating proteins (GAP)domain is structurally homologus to other Rho and GAP domains such as those found in p190RhoGAP, p50RhoGAP and BCR (1). It is shown that β2-chimaerin accelerates GTP hydrolysis from the small G-protein Rac1, leading to the conversion to the inactive GDP-bound form of Rac (2). Rac1 plays a fundamental role in the control of actin cytoskeleton reorganization, migration, adhesion, transcription, and cell cycle progression (3). An N-terminal SH2 domain in both alfa and beta chimaerins interacts with phosphotyrosine containing proteins while a C-terminalGAP doamain is structurally homologuas to other RhoGAP domains such as those found in other GAP proteins. The beta 2 chimaerins have been shown to accelerate GTP hydrolysis from the small G-protein Rac1 and converts it to inactive GDP bound form. Since Rac1 plays an important role in actin cytoskeletal reorganization, adhesion, migration, transcription and cell cycle progression and chimaerins regulate the activity of Rac1, it is conceivable that chimaerins could play an equally important negatively regulated role in concert with Rac1 responses (4).
Chimaerin beta 2 is a 468 amino acid protein (MW 50kDa) protein and chimaerin alfa is a 433 amino acid (45kDa) in size. Chimaerins are expressed in majority of cells and are important regulators of divergence in diacylglycerol (DAG) signaling downstream of tyrosine kinase receptor via a protein kinase C independent mechanisms (1). Beta 2-Chimaerins also undergo phosphorylation to regulate their activity. Alfa-chimaerins are alternatively spliced to give two variants (alfa1 and alfa 2-chiamerins), the alfa-1 is a GTPase activating protein for Ras related p21rac while alfa 2-chimaerin exhibted Rac-GAP activity which is stimulated by phosophotidyl serine. Discrete expression of alfa 1-chimaerin and alfa 2-chimerins are seen in brain, alfa 1-chimaerin is expressed in Purkinje cell while both alfa 1 and alfa 2 are expressed in neurons of the cerebral cortex, hippocampus, and thalamus. The alfa 2 variant is expressed only in testis, in early pachytene spermatocytes (4).

General readings

Caloca,M.J., (2008) Cell. Signal. 20 (4), 758-770

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ARHGAP2 / CHN1 antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-CHN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505072) Mouse IgG2b OTI1C9 Purified Hu F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ARHGAP2 / CHN1 antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-CHN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505073) Mouse IgG1 OTI1B7 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ARHGAP2 / CHN1 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CHN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505103) Mouse IgG1 OTI2D10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ARHGAP2 / CHN1 antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CHN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505109) Mouse IgG1 OTI3C6 Purified Can, Hu, Mky, Ms, Rt P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

ARHGAP2 / CHN1 antibody

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-CHN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505110) Mouse IgG2a OTI2G6 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ARHGAP2 / CHN1 antibody

CHN1 monoclonal antibody (M03), clone 3A3. Western Blot analysis of CHN1 expression in NIH/3T3(Cat # L018V1 ). Mouse IgG2a 3A3 Purified Hu, Ms, Rt E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ARHGAP2 / CHN1 antibody

WB Suggested Anti-CHN1 Antibody Titration: 0.2-1 ug/ml; Positive Control: Jurkat cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ye, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

ARHGAP2 / CHN1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHN1 (RC222163, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHN1. Mouse IgG1 OTI2A1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ARHGAP2 / CHN1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHN1 (RC222163, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHN1. Mouse IgG1 OTI4D1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ARHGAP2 / CHN1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHN1 (RC222163, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHN1. Mouse IgG2a OTI2E11 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ARHGAP2 / CHN1 control peptide

ARHGAP2 / CHN1
0.25 mg / €250.00
  Acris Antibodies GmbH

ARHGAP2 / CHN1 (Alpha-2) antibody

ARHGAP2 / CHN1 Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

ARHGAP2 / CHN1 antibody

Western Blot analysis of CHN1 expression in transfected 293T cell line (H00001123-T01) by CHN1 MaxPab polyclonal antibody.

Lane 1: CHN1 transfected lysate(50.49 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

ARHGAP2 / CHN1 antibody

Western Blot analysis of CHN1 expression in transfected 293T cell line (H00001123-T01) by CHN1 MaxPab polyclonal antibody.

Lane 1: CHN1 transfected lysate(50.49 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

ARHGAP2 / CHN1 antibody

Western Blot analysis of CHN1 expression in transfected 293T cell line (H00001123-T01) by CHN1 MaxPab polyclonal antibody.

Lane 1: CHN1 transfected lysate(53.20 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

ARHGAP2 / CHN1 antibody

ARHGAP2 / CHN1 Mouse IgG1 3F8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ARHGAP2 / CHN1 antibody

ARHGAP2 / CHN1 Rabbit Hu, Ms, Rt WB
0.1 mg / €470.00
  Abnova Taiwan Corp.

ARHGAP2 / CHN1 antibody

Human Jurkat; WB Suggested Anti-CHN1 Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysate; CHN1 antibody - middle region (ARP51973_P050) in Human Jurkat cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Chk, Dros, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

ARHGAP2 / CHN1 (C-term) antibody

Human Fetal Thymus; WB Suggested Anti-CHN1 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Fetal Thymus; CHN1 antibody - C-terminal region (ARP51974_P050) in Human Fetal Thymus cells using Western Blot Rabbit Aff - Purified Hu
50 µg / €350.00
  AVIVA Systems Biology

ARHGAP2 / CHN1 antibody

ARHGAP2 / CHN1 Rabbit IgG Hu WB
0.1 ml / €340.00
  Novus Biologicals Inc.

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