ARHGAP2 / CHN1 antibody

Principal name

ARHGAP2 / CHN1 antibody

Alternative names for ARHGAP2 / CHN1 antibody

N-chimaerin, Alpha-chimerin, A-chimaerin, Rho GTPase-activating protein 2

SwissProt ID

P15882 (Human), P15882-2, P30337 (Rat), Q17QN0 (Bovin), Q91V57 (Mouse)

Gene ID

1123 (CHN1), 108699 (Chn1), 84030

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Dros (Drosophila), Ze (Zebrafish), African clawed frog, Can (Canine), Mky (Monkey), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Ye (Yeast)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Flow Cytometry (F), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of ARHGAP2 / CHN1 antibody

Chimaerins are members of a Rho GTPase-activating proteins (GAPs) that comprises of beta1, alfa1 and alfa 2-chimaerins and the alternatively spliced variants from the beta and alfa-chimaerin genes. These proteins bind to diacylglycerol and phorbol esters like protein kinaseC. They all have a common single C1 domain which is capable of binding phorbol esters and other DAG-mimetics with low nonomolar affinity in the presence of acidic phospholipids. There are two types of chimaerins, alfa2 and beta2 Chimaerins both exhibit an N-terminal SH2 binding domain that interacts with phosphotyrosine containing proteins. The C-terminal chimaerin GTPase activating proteins (GAP)domain is structurally homologus to other Rho and GAP domains such as those found in p190RhoGAP, p50RhoGAP and BCR (1). It is shown that β2-chimaerin accelerates GTP hydrolysis from the small G-protein Rac1, leading to the conversion to the inactive GDP-bound form of Rac (2). Rac1 plays a fundamental role in the control of actin cytoskeleton reorganization, migration, adhesion, transcription, and cell cycle progression (3). An N-terminal SH2 domain in both alfa and beta chimaerins interacts with phosphotyrosine containing proteins while a C-terminalGAP doamain is structurally homologuas to other RhoGAP domains such as those found in other GAP proteins. The beta 2 chimaerins have been shown to accelerate GTP hydrolysis from the small G-protein Rac1 and converts it to inactive GDP bound form. Since Rac1 plays an important role in actin cytoskeletal reorganization, adhesion, migration, transcription and cell cycle progression and chimaerins regulate the activity of Rac1, it is conceivable that chimaerins could play an equally important negatively regulated role in concert with Rac1 responses (4).
Chimaerin beta 2 is a 468 amino acid protein (MW 50kDa) protein and chimaerin alfa is a 433 amino acid (45kDa) in size. Chimaerins are expressed in majority of cells and are important regulators of divergence in diacylglycerol (DAG) signaling downstream of tyrosine kinase receptor via a protein kinase C independent mechanisms (1). Beta 2-Chimaerins also undergo phosphorylation to regulate their activity. Alfa-chimaerins are alternatively spliced to give two variants (alfa1 and alfa 2-chiamerins), the alfa-1 is a GTPase activating protein for Ras related p21rac while alfa 2-chimaerin exhibted Rac-GAP activity which is stimulated by phosophotidyl serine. Discrete expression of alfa 1-chimaerin and alfa 2-chimerins are seen in brain, alfa 1-chimaerin is expressed in Purkinje cell while both alfa 1 and alfa 2 are expressed in neurons of the cerebral cortex, hippocampus, and thalamus. The alfa 2 variant is expressed only in testis, in early pachytene spermatocytes (4).

General readings

Caloca,M.J., (2008) Cell. Signal. 20 (4), 758-770

  1. Quick-Filters:
  2. Highly Validated (5)

Items 1 to 20 of 24 total

per page
Page:
  1. 1
  2. 2

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ARHGAP2 / CHN1 antibody

  CHN1 mouse monoclonal antibody, clone OTI1C9 (formerly 1C9)  
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-CHN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505072) Mouse IgG2b OTI1C9 Purified Hu F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ARHGAP2 / CHN1 antibody

  CHN1 mouse monoclonal antibody, clone OTI1B7 (formerly 1B7)  
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-CHN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505073) Mouse IgG1 OTI1B7 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ARHGAP2 / CHN1 antibody

  CHN1 mouse monoclonal antibody, clone OTI2D10 (formerly 2D10)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CHN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505103) Mouse IgG1 OTI2D10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ARHGAP2 / CHN1 antibody

  CHN1 mouse monoclonal antibody, clone OTI3C6 (formerly 3C6)  
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CHN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505109) Mouse IgG1 OTI3C6 Purified Can, Hu, Mky, Ms, Rt P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

ARHGAP2 / CHN1 antibody

  CHN1 mouse monoclonal antibody, clone OTI2G6 (formerly 2G6)  
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-CHN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505110) Mouse IgG2a OTI2G6 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ARHGAP2 / CHN1 antibody

  Rabbit Polyclonal Anti-CHN1 Antibody  
WB Suggested Anti-CHN1 Antibody Titration: 0.2-1 ug/ml; Positive Control: Jurkat cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ye, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

ARHGAP2 / CHN1 antibody

  CHN1 mouse monoclonal antibody, clone OTI2A1 (formerly 2A1)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHN1 (RC222163, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHN1. Mouse IgG1 OTI2A1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ARHGAP2 / CHN1 antibody

  CHN1 mouse monoclonal antibody, clone OTI4D1 (formerly 4D1)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHN1 (RC222163, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHN1. Mouse IgG1 OTI4D1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ARHGAP2 / CHN1 antibody

  CHN1 mouse monoclonal antibody, clone OTI2E11 (formerly 2E11)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHN1 (RC222163, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHN1. Mouse IgG2a OTI2E11 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ARHGAP2 / CHN1 (transcript variant 2)

  Recombinant protein of human chimerin (chimaerin) 1 (CHN1), transcript variant 2  
ARHGAP2 / CHN1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

ARHGAP2 / CHN1 (transcript variant 1)

  Recombinant protein of human chimerin (chimaerin) 1 (CHN1), transcript variant 1  
ARHGAP2 / CHN1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Lysates

Catalog No.    

CHN1 overexpression lysate

CHN1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, CHN1 (mGFP-tagged) - Human chimerin (chimaerin) 1 (CHN1), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, CHN1 (mGFP-tagged) - Human chimerin (chimaerin) 1 (CHN1), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, CHN1 (mGFP-tagged) - Human chimerin (chimaerin) 1 (CHN1), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, CHN1 (mGFP-tagged) - Human chimerin (chimaerin) 1 (CHN1), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €1,030.00
  OriGene Technologies, Inc.

Lenti ORF particles, CHN1 (Myc-DDK tagged) - Human chimerin (chimaerin) 1 (CHN1), transcript variant 1 , 200 uL, >10^7 TU/mL

Lenti ORF particles, CHN1 (Myc-DDK tagged) - Human chimerin (chimaerin) 1 (CHN1), transcript variant 1 , 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

Lenti ORF particles, CHN1 (Myc-DDK tagged) - Human chimerin (chimaerin) 1 (CHN1), transcript variant 2 , 200 uL, >10^7 TU/mL

Lenti ORF particles, CHN1 (Myc-DDK tagged) - Human chimerin (chimaerin) 1 (CHN1), transcript variant 2 , 200 uL, >10^7 TU/mL  
200 µl / €920.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Chn1 (GFP-tagged) - Mouse chimerin (chimaerin) 1 (Chn1), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, Chn1 (GFP-tagged) - Mouse chimerin (chimaerin) 1 (Chn1), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, Chn1 (GFP-tagged) - Mouse chimerin (chimaerin) 1 (Chn1), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, Chn1 (GFP-tagged) - Mouse chimerin (chimaerin) 1 (Chn1), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, Chn1 (GFP-tagged) - Mouse chimerin (chimaerin) 1 (Chn1), transcript variant 5, 200 uL, >10^7 TU/mL

Lenti ORF particles, Chn1 (GFP-tagged) - Mouse chimerin (chimaerin) 1 (Chn1), transcript variant 5, 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, Chn1 (Myc-DDK-tagged) - Mouse chimerin (chimaerin) 1 (Chn1), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, Chn1 (Myc-DDK-tagged) - Mouse chimerin (chimaerin) 1 (Chn1), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

Items 1 to 20 of 24 total

per page
Page:
  1. 1
  2. 2
  • LinkedIn