ARHGAP3 / CHN2 antibody

Principal name

ARHGAP3 / CHN2 antibody

Alternative names for ARHGAP3 / CHN2 antibody

Beta-chimaerin, BCH, Beta-chimerin, Rho GTPase-activating protein 3

SwissProt ID

P52757 (Human), Q03070 (Rat), Q80XD1 (Mouse)

Gene ID

1124 (CHN2)

Available reactivities

Bov (Bovine), Chk (Chicken), Hu (Human), Ms (Mouse), Rt (Rat), African clawed frog, Dros (Drosophila), Ze (Zebrafish), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Goat

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E)

Background of ARHGAP3 / CHN2 antibody

Chimaerins are members of a Rho GTPase-activating proteins (GAPs) that comprises of beta1, alfa1 and alfa 2-chimaerins and the alternatively spliced variants from the beta and alfa-chimaerin genes. These proteins bind to diacylglycerol and phorbol esters like protein kinaseC. They all have a common single C1 domain which is capable of binding phorbol esters and other DAG-mimetics with low nanomolar affinity in the presence of acidic phospholipids. There are two types of chimaerins, alfa2 and beta2 Chimaerins both exhibit an N-terminal SH2 binding domain that interacts with phosphotyrosine containing proteins. The C-terminal chimaerin GTPase activating proteins (GAP)domain is structurally homologus to other Rho and GAP domains such as those found in p190RhoGAP, p50RhoGAP and BCR (1). It is shown that β2-chimaerin accelerates GTP hydrolysis from the small G-protein Rac1, leading to the conversion to the inactive GDP-bound form of Rac (2). Rac1 plays a fundamental role in the control of actin cytoskeleton reorganization, migration, adhesion, transcription, and cell cycle progression (3). An N-terminal SH2 domain in both alfa and beta chimaerins interacts with phosphotyrosine containing proteins while a C-terminalGAP doamain is structurally homologuas to other RhoGAP domains such as those found in other GAP proteins. The beta 2 chimaerins have been shown to accelerate GTP hydrolysis from the small G-protein Rac1 and converts it to inactive GDP bound form. Since Rac1 plays an important role in actin cytoskeletal reorganization, adhesion, migration, transcription and cell cycle progression and chimaerins regulate the activity of Rac1, it is conceivable that chimaerins could play an equally important negatively regulated role in concert with Rac1 responses (4).
Chimaerin beta 2 is a 468 amino acid protein (MW 50kDa) protein and chimaerin alfa is a 433 amino acid (45kDa) in size. Chimaerins are expressed in majority of cells and are important regulators of divergence in diacylglycerol (DAG) signaling downstream of tyrosine kinase receptor via a protein kinase C independent mechanisms (1). Beta 2-Chimaerins also undergo phosphorylation to regulate their activity. Alfa-chimaerins are alternatively spliced to give two variants (alfa1 and alfa 2-chiamerins), the alfa-1 is a GTPase activating protein for Ras related p21rac while alfa 2-chimaerin exhibted Rac-GAP activity which is stimulated by phosophotidyl serine. Discrete expression of alfa 1-chimaerin and alfa 2-chimerins are seen in brain, alfa 1-chimaerin is expressed in Purkinje cell while both alfa 1 and alfa 2 are expressed in neurons of the cerebral cortex, hippocampus, and thalamus. The alfa 2 variant is expressed only in testis, in early pachytene spermatocytes (4).

General readings

Takefuji, M., et al. J. Hum. Genet. 55(1):42-49 (2010)
Suliman, S.G., et al. Diabetes 58(12):2954-2961 (2009)
Siliceo, M., et al. J. Biol. Chem. 284(17):11354-11363 (2009)

14 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ARHGAP3 / CHN2 antibody

WB Suggested Anti-CHN2 Antibody Titration: 0.2-1 ug/ml; Positive Control: Human Liver Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

ARHGAP3 / CHN2 antibody

WB Suggested Anti-CHN2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Human Thymus Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

ARHGAP3 / CHN2 control peptide

ARHGAP3 / CHN2 WB
0.25 mg / €420.00
  Acris Antibodies GmbH

ARHGAP3 / CHN2 (beta-2) (163-178) antibody

ARHGAP3 / CHN2 Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

ARHGAP3 / CHN2 (C-term) antibody

CHN2 antibody cat.-No AP31984PU-N staining of paraffin embedded Human Cortex at 3.8 µg/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat Aff - Purified Bov, Can, Hu, Ms E, P
0.1 mg / €340.00
  Acris Antibodies GmbH

ARHGAP3 / CHN2 (Center) antibody

Western blot analysis of CHN2 (arrow) in Hela cell line lysates (35ug/lane) using ARHGAP3/CHN2 antibody Cat.-No. AP50901PU-N. Rabbit Ig Aff - Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

ARHGAP3 / CHN2 antibody

Human Liver; WB Suggested Anti-CHN2 Antibody Titration: 0.2-1 ug/ml. Positive Control: Human Liver; CHN2 antibody - N-terminal region (ARP51862_P050) in Human Liver cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Chk, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

ARHGAP3 / CHN2 antibody

Human Thymus; WB Suggested Anti-CHN2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Thymus; CHN2 antibody - N-terminal region (ARP51863_P050) in Human Thymus cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Chk, Dros, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

ARHGAP3 / CHN2 antibody

ARHGAP3 / CHN2 Goat IgG Hu E, P, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

ARHGAP3 / CHN2 antibody

Western Blot: Chimaerin 2 Antibody [NBP1-55094] - This Anti-CHN2 antibody was used in Western Blot of Fetal Liver tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €440.00
  Novus Biologicals Inc.

ARHGAP3 / CHN2 antibody

Western Blot: Chimaerin 2 Antibody [NBP1-55257] - This Anti-CHN2 antibody was used in Western Blot of Fetal Thymus   tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €440.00
  Novus Biologicals Inc.

ARHGAP3 / CHN2 antibody

Immunohistochemistry-Paraffin: Chimaerin 2 Antibody [NBP1-90108] - Immunohistochemical staining of human colon shows strong cytoplasmic positivity in granular pattern in glandular cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ARHGAP3 / CHN2 (transcript variant 2)

ARHGAP3 / CHN2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

CHN2 overexpression lysate

CHN2 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

14 Item(s)

per page
  • LinkedIn