ARHGDIA / GDIA1 antibody

Principal name

ARHGDIA / GDIA1 antibody

Alternative names for ARHGDIA / GDIA1 antibody

Rho GDI 1, Rho-GDI alpha, RhoGDI1, Rho GDP-dissociation inhibitor 1

SwissProt ID

P19803 (Bovin), P52565 (Human), Q5XI73 (Rat), Q99PT1 (Mouse)

Gene ID

396 (ARHGDIA), 360678 (Arhgdia), 192662

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Mky (Monkey), Bov (Bovine)

Available hosts

Rabbit, Mouse, Goat

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), EPair for Elisa (EPair), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)

Background of ARHGDIA / GDIA1 antibody

Rho family small GTPases, including Rho, Rac and cdc42, act as molecular switches, regulating processes such as cell migration, adhesion, proliferation and differentiation. They are activated by guanine nucleotide exchange factors (GEFs), which catalyze the exchange of bound GDP for GTP, and inhibited by GTPase activating proteins (GAPs), which catalyze the hydrolysis of GTP to GDP. A third level of regulation is provided by the stoichiometric binding of Rho GDP dissociation inhibitor (RhoGDI). RhoGDI affects Rho activity by inhibiting nucleotide exchange and membrane association, regulating activity and localization. The inhibitory and shuttling functions of RhoGDI have been uncoupled using mutant forms of RhoGDI. Phosphorylation of GDIs and/or GTPases can modulate their affinity for each other and, therefore, GTPase mediated sigling. PAK1 phosphorylation of RhoGDI at serines 101 and 174 causes release and activation of Rac1, but not RhoA.

General readings

1. Ronneburg H, Span PN, Kantelhardt E, Dittmer A, Schunke D, Holzhausen HJ, Sweep FC, Dittmer J, Rho GDP dissociation inhibitor alpha expression correlates with the outcome of CMF treatment in invasive ductal breast cancer. International journal of oncology 2010 Feb 36 (2): 379-86.
PMID: 20043072

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ARHGDIA / GDIA1 antibody

  ARHGDIA (RhoGDI) mouse monoclonal antibody, clone OTI1F2 (formerly 1F2)  
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ARHGDIA monoclonal antibody. Mouse IgG1 OTI1F2 Purified Can, Hu, Mky, Rt F, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

ARHGDIA / GDIA1 antibody

  anti-ARHGDIA (RhoGDI) mouse monoclonal antibody, clone OTI1A7 (formerly 1A7)  
Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-ARHGDIA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501120, Dilution 1:50) Mouse IgG1 OTI1A7 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ARHGDIA / GDIA1 antibody

  Goat Anti-ARHGDIA Antibody  
(1ug/ml) staining of Mouse Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ARHGDIA / GDIA1 antibody

  Anti-ARHGDIA Rabbit Polyclonal Antibody  
Predicted band size: 23 kDa . Positive control: Jurkat, 293, A431, Hela and HL60 cell, mouse pancreas tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: Jurkat cell lysate Lane2: 293 cel lysate Lane3: A431 cell lysate Lane4: Hela cell lysate Lane5: HL60 cell lysate Lane6: Mouse pancreas tissue lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ARHGDIA / GDIA1 antibody

  Rabbit anti-ARHGDIA Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using ARHGDIA antibody. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ARHGDIA / GDIA1 antibody

  Rabbit Polyclonal Anti-ARHGDIA Antibody  
Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane 1-6: Mouse spleen tissue, A375 cells, A549 cells, HT29 cells, mouse lung tissue, Hela cells, Primary antibody: (ARHGDIA Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ARHGDIA / GDIA1 antibody

  Anti-CD36 mouse mAb, clone 3F4, Bi, clone OTI3F4 (formerly 3F4)  
HEK293T cells were transfected with pCMV6-ENTRY control (Lane 1) or pCMV6-ENTRY CD36 (Lane 2) plasmids for 48 hrs. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD36-biotin and Streptavidin-HRP. Mouse OTI3F4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ARHGDIA / GDIA1 antibody

  Anti-CD36 mouse mAb, clone 3F4, HRP , clone OTI3F4 (formerly 3F4)  
HEK293T cells were transfected with pCMV6-ENTRY control (Lane 1) or pCMV6-ENTRY CD36 (Lane 2) plasmids for 48 hrs. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD36- HRP. Mouse OTI3F4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ARHGDIA / GDIA1 antibody

  Anti-CD36 mouse mAb, clone 4H7, Bi, clone OTI4H7 (formerly 4H7)  
HEK293T cells were transfected with pCMV6-ENTRY control (Lane 1) or pCMV6-ENTRY CD36 (Lane 2) plasmids for 48 hrs. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD36-biotin and Streptavidin-HRP. Mouse OTI4H7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ARHGDIA / GDIA1 antibody

  Anti-CD36 mouse mAb, clone 4H7, HRP , clone OTI4H7 (formerly 4H7)  
HEK293T cells were transfected with pCMV6-ENTRY control (Lane 1) or pCMV6-ENTRY CD36 (Lane 2) plasmids for 48 hrs. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD36- HRP. Mouse OTI4H7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ARHGDIA / GDIA1 (C-term) antibody

Western blot analysis of ARHGDIA Antibody (C-term) (AP17127PU-N) in A375,HL-60,Ramos cell line lysates (35 µg/lane). ARHGDIA (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

ARHGDIA / GDIA1 pSer174 antibody

Immunohistochemistry analysis of paraffin-embedded human cervix carcinoma, using ARHGDIA (Phospho-Ser174) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €230.00
  Assay Biotech

ARHGDIA / GDIA1 pSer174 antibody

Immunohistochemistry analysis of paraffin-embedded human cervix carcinoma, using ARHGDIA (Phospho-Ser174) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €330.00
  Assay Biotech

ARHGDIA / GDIA1 antibody

Immunohistochemistry analysis of paraffin-embedded human cervix carcinoma tissue, using ARHGDIA (Ab-174) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P
50 µg / €230.00
  Assay Biotech

ARHGDIA / GDIA1 antibody

Immunohistochemistry analysis of paraffin-embedded human cervix carcinoma tissue, using ARHGDIA (Ab-174) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

Arhgdia - mouse gene knockout kit via CRISPR

Arhgdia - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Arhgdia - mouse gene knockout kit via CRISPR

Arhgdia - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Arhgdia - mouse gene knockout kit via CRISPR

Arhgdia - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Arhgdia - mouse gene knockout kit via CRISPR

Arhgdia - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ARHGDIA / GDIA1

  Recombinant protein of human Rho GDP dissociation inhibitor (GDI) alpha (ARHGDIA)  
ARHGDIA / GDIA1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

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