ARHGDIA / GDIA1 antibody

Principal name

ARHGDIA / GDIA1 antibody

Alternative names for ARHGDIA / GDIA1 antibody

Rho GDI 1, Rho-GDI alpha, RhoGDI1, Rho GDP-dissociation inhibitor 1

SwissProt ID

P19803 (Bovin), P52565 (Human), Q5XI73 (Rat), Q99PT1 (Mouse)

Gene ID

396 (ARHGDIA), 192662 (Arhgdia)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Mky (Monkey), Chk (Chicken)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunoprecipitation (IP), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)

Background of ARHGDIA / GDIA1 antibody

Rho family small GTPases, including Rho, Rac and cdc42, act as molecular switches, regulating processes such as cell migration, adhesion, proliferation and differentiation. They are activated by guanine nucleotide exchange factors (GEFs), which catalyze the exchange of bound GDP for GTP, and inhibited by GTPase activating proteins (GAPs), which catalyze the hydrolysis of GTP to GDP. A third level of regulation is provided by the stoichiometric binding of Rho GDP dissociation inhibitor (RhoGDI). RhoGDI affects Rho activity by inhibiting nucleotide exchange and membrane association, regulating activity and localization. The inhibitory and shuttling functions of RhoGDI have been uncoupled using mutant forms of RhoGDI. Phosphorylation of GDIs and/or GTPases can modulate their affinity for each other and, therefore, GTPase mediated sigling. PAK1 phosphorylation of RhoGDI at serines 101 and 174 causes release and activation of Rac1, but not RhoA.

General readings

Ishizaki,H., et al. Defective chemokine-directed lymphocyte migration and development in the absence of Rho guanosine diphosphate-dissociation inhibitors alpha and beta. J. Immunol. 177 (12), 8512-8521 (2006).
Céline DerMardirossian et al. Phosphorylation of RhoGDI by Src Regulates Rho GTPase Binding and Cytosol-Membrane Cycling. Mol. Biol. Cell. 17 (11). 4760-4768 (2006).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ARHGDIA / GDIA1 antibody

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ARHGDIA monoclonal antibody. Mouse IgG1 OTI1F2 Purified Can, Hu, Mky, Rt F, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

ARHGDIA / GDIA1 antibody

Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-ARHGDIA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501120, Dilution 1:50) Mouse IgG1 OTI1A7 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ARHGDIA / GDIA1 antibody

ARHGDIA MaxPab rabbit polyclonal antibody. Western Blot analysis of ARHGDIA expression in mouse spleen. Rabbit Serum Hu, Ms E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

ARHGDIA / GDIA1 antibody

ARHGDIA MaxPab rabbit polyclonal antibody. Western Blot analysis of ARHGDIA expression in mouse spleen. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

ARHGDIA / GDIA1 antibody

(1ug/ml) staining of Mouse Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ARHGDIA / GDIA1 antibody

Predicted band size: 23 kDa . Positive control: Jurkat, 293, A431, Hela and HL60 cell, mouse pancreas tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: Jurkat cell lysate Lane2: 293 cel lysate Lane3: A431 cell lysate Lane4: Hela cell lysate Lane5: HL60 cell lysate Lane6: Mouse pancreas tissue lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ARHGDIA / GDIA1 antibody

Western blot analysis of extracts of various cell lines, using ARHGDIA antibody. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ARHGDIA / GDIA1 antibody

HEK293T cells were transfected with pCMV6-ENTRY control (Lane 1) or pCMV6-ENTRY CD36 (Lane 2) plasmids for 48 hrs. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD36-biotin and Streptavidin-HRP. Mouse OTI3F4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ARHGDIA / GDIA1 antibody

HEK293T cells were transfected with pCMV6-ENTRY control (Lane 1) or pCMV6-ENTRY CD36 (Lane 2) plasmids for 48 hrs. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD36- HRP. Mouse OTI3F4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ARHGDIA / GDIA1 antibody

HEK293T cells were transfected with pCMV6-ENTRY control (Lane 1) or pCMV6-ENTRY CD36 (Lane 2) plasmids for 48 hrs. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD36-biotin and Streptavidin-HRP. Mouse OTI4H7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ARHGDIA / GDIA1 antibody

HEK293T cells were transfected with pCMV6-ENTRY control (Lane 1) or pCMV6-ENTRY CD36 (Lane 2) plasmids for 48 hrs. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD36- HRP. Mouse OTI4H7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ARHGDIA / GDIA1 (C-term) antibody

Western blot analysis of ARHGDIA Antibody (C-term) (AP17127PU-N) in A375,HL-60,Ramos cell line lysates (35 µg/lane). ARHGDIA (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

ARHGDIA / GDIA1 antibody

ARHGDIA / GDIA1 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

ARHGDIA / GDIA1 antibody

ARHGDIA polyclonal antibody (A02), Lot # 050921JC01 Western Blot analysis of ARHGDIA expression in SJCRH30 ( Cat # L027V1 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

ARHGDIA / GDIA1 antibody

Western Blot analysis of ARHGDIA expression in transfected 293T cell line (H00000396-T01) by ARHGDIA MaxPab polyclonal antibody.

Lane1:ARHGDIA transfected lysate(22.55 KDa).
Lane 2:Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

ARHGDIA / GDIA1 antibody

Western Blot analysis of ARHGDIA expression in transfected 293T cell line (H00000396-T02) by ARHGDIA MaxPab polyclonal antibody.

Lane 1: ARHGDIA transfected lysate(22.44 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

ARHGDIA / GDIA1 antibody

Immunoprecipitation of ARHGDIA transfected lysate using anti-ARHGDIA MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with ARHGDIA MaxPab mouse polyclonal antibody (B01) (H00000396-B01). Rabbit Serum Hu, Ms E(detection), IP
0.1 ml / €380.00
  Abnova Taiwan Corp.

ARHGDIA / GDIA1 antibody

Western Blot analysis of ARHGDIA expression in transfected 293T cell line (H00000396-T03) by ARHGDIA MaxPab polyclonal antibody.

Lane 1: ARHGDIA transfected lysate(22.55 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

ARHGDIA / GDIA1 antibody

Detection limit for recombinant GST tagged ARHGDIA is approximately 0.3ng/ml as a capture antibody. Mouse IgG1 1E12-2G11 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

ARHGDIA / GDIA1 antibody

Detection limit for recombinant GST tagged ARHGDIA is approximately 0.3ng/ml as a capture antibody. Mouse IgG1 1G5-2F3 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

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