ARIH2 / TRIAD1 antibody

Principal name

ARIH2 / TRIAD1 antibody

Alternative names for ARIH2 / TRIAD1 antibody

ARI2, HT005, Protein ariadne-2 homolog

SwissProt ID

O95376 (Human), Q9Z1K6 (Mouse)

Gene ID

10425 (ARIH2)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Chk (Chicken), Rt (Rat), African clawed frog, Por (Porcine), Xen (Xenopus), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P), Dot blot (Dot)

Background of ARIH2 / TRIAD1 antibody

**Please note, this product is one of a range of Investigative Grade antibodies, made against targets that have limited or no commercial antibodies available to them and for which there are no data on the expression of the protein in the range of common cell lines and tissues available to us. These antibodies are affinity purified using their peptide immunogen and are known to give low background staining in a western blot. However no additional claims are made for their ability to recognise native protein in any application. ARIH2 might act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, such as UBE2L3/UBCM4, and then transfers it to substrates.

General readings

"The E3 ubiquitin-protein ligase Triad1 inhibits clonogenic growth of primary myeloid progenitor cells." Marteijn J.A., van Emst L., Erpelinck-Verschueren C.A., Nikoloski G., Menke A., de Witte T., Loewenberg B., Jansen J.H., van der Reijden B.A. Blood 106:4114-4123(2005)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ARIH2 / TRIAD1 (C-term) antibody

TA305751 (2µg/ml) staining of Hela (A) and K562 (B) nuclear lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt ICC/IF, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ARIH2 / TRIAD1 antibody

WB Suggested Anti-ARIH2 Antibody Titration: 2.5 ug/ml; Positive Control: Jurkat cell lysateThere is BioGPS gene expression data showing that ARIH2 is expressed in Jurkat Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ARIH2 / TRIAD1 antibody

Host: Rabbit; Target Name: Arih2; Sample Tissue: Rat Testis lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Hu, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

ARIH2 / TRIAD1 (N-term) antibody

WB Suggested Anti-ARIH2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human heart Rabbit Aff - Purified Hu, Ms, Por, Rt, Xen WB
50 µg / €325.00
  OriGene Technologies, Inc.

ARIH2 / TRIAD1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARIH2 (RC201846, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARIH2. Mouse IgG1 OTI3F9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ARIH2 / TRIAD1 antibody

ARIH2 / TRIAD1 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

ARIH2 / TRIAD1 antibody

Immunofluorescence of purified MaxPab antibody to ARIH2 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

ARIH2 / TRIAD1 antibody

Immunofluorescence of purified MaxPab antibody to ARIH2 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

ARIH2 / TRIAD1 antibody

ARIH2 / TRIAD1 Mouse IgM 1C3 Ascites Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

ARIH2 / TRIAD1 antibody

ARIH2 / TRIAD1 Goat Hu, Ms E
0.1 mg / €400.00
  Abnova Taiwan Corp.

ARIH2 / TRIAD1 antibody

Human Lung; ARIH2 antibody - N-terminal region (ARP34418_T100) in Human Lung cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt P, WB
0.1 mg / €310.00
  AVIVA Systems Biology

ARIH2 / TRIAD1 (N-term) antibody

ARIH2 / TRIAD1 Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

ARIH2 / TRIAD1 antibody

ARIH2 / TRIAD1 Goat IgG Aff - Purified Hu, Ms E, IP
0.1 mg / €400.00
  Novus Biologicals Inc.

ARIH2 / TRIAD1 antibody

Western Blot: ARIH2 Antibody [NBP1-31044] - Western blot analysis of ARIH2 Antibody on: A: H1299 7.5% SDS PAGE diluted at 1:1000 Rabbit IgG Hu Dot, ICC/IF, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

ARIH2 / TRIAD1 antibody

Western Blot: ARIH2 Antibody [NBP1-49913] - Detection of Human and Mouse Triad1 by Western Blot (h&m) and Immunoprecipitation (h). Samples:  Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells.  Antibodies: Affinity purified rabbit anti-Triad1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate.  Triad1 was also immunoprecipitated by rabbit anti-Triad1 antibody, which recognizes an upstream epitope.  For blotting immunoprecipitated Triad1 was used.  Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B). Rabbit Aff - Purified Hu, Ms IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ARIH2 / TRIAD1

ARIH2 / TRIAD1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

ARIH2 / TRIAD1

ARIH2 / TRIAD1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

ARIH2 / TRIAD1

ARIH2 / TRIAD1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

ARIH2 / TRIAD1

ARIH2 / TRIAD1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

ARIH2 / TRIAD1

ARIH2 / TRIAD1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

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