ARL3 antibody

Principal name

ARL3 antibody

Alternative names for ARL3 antibody

ARFL3, ADP-ribosylation factor-like protein 3

SwissProt ID

P36405 (Human), P37996 (Rat), Q2TBW6 (Bovin), Q52NJ4 (Pig), Q9WUL7 (Mouse)

Gene ID

403 (ARL3)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P), Western blot / Immunoblot (WB), ELISA (detection) (E(detection))

Background of ARL3 antibody

ADP-ribosylation factors (ARFs) are low molecular weight GTP-binding proteins belonging to the RAS superfamily. The predicted 182-amino acid ARL3 (ADP-ribosylation like factor) protein shares 97% amino acid identity with rat ARLl3 and 43% identity with human ARF1. Like the ARFs, ARL3 has a glycine at position 2, the site of N myristoylation, and lacks cysteine residues near the C terminus, which are found in other members of the RAS family. Northern blot analysis detected a 1-kb ARL3 transcript in all tissues tested, with highest expression in heart and lung, and lower expression in brain, liver, kidney, ovary, and testis. A 5.5-kb transcript was also detected in most tissues, with highest expression in brain. Immunoblot analysis detected ARL3 in human tumor cell lines but not in normal rodent cells. Although ARL3 binds GTP, it is devoid of activity in the cholera toxin-dependent ADP-ribosylation of Gs, and is therefore classified as an ARF-like protein.

General readings

Bartolini F. et al. (2002) J Biol Chem. 277:14629-14634.Hillig R.C. et al. (2001) Structure. 8:1239-1245.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ARL3 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-ARL3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA803087) Mouse IgG2a OTI1C6 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ARL3 antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ARL3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA803091) Mouse IgG2a OTI2A9 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ARL3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARL3 (RC203469, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARL3. Mouse IgG2b OTI7E5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ARL3 antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ARL3 mouse monoclonal antibody. (TA803086) Dilution: 1:150 Mouse IgG2a OTI3A10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ARL3 antibody

Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-ARL3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA803088) Mouse IgG2a OTI2G7 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ARL3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARL3 (RC203469, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARL3. Mouse IgG1 OTI3E1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ARL3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARL3 (RC203469, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARL3. Mouse IgG2b OTI5E6 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ARL3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARL3 (RC203469, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARL3. Mouse IgG2a OTI3C6 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ARL3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARL3 (RC203469, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARL3. Mouse IgG2a OTI3D3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ARL3 (N-term) antibody

Western blot analysis using anti-ARL3 (N-term) Pab (Cat.#AP12134PU-N). Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

ARL3 (C-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

ARL3 antibody

ARL3 Mouse Hu WB
50 µg / €370.00
  Abnova Taiwan Corp.

ARL3 antibody

Immunofluorescence of purified MaxPab antibody to ARL3 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

ARL3 antibody

Immunofluorescence of purified MaxPab antibody to ARL3 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

ARL3 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with ARL3 polyclonal antibody ( Cat # PAB4262 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma . Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

ARL3 (N-term) antibody

ARL3 Rabbit Aff - Purified Hu
50 µg / €350.00
  AVIVA Systems Biology

ARL3 antibody

ARL3 Rabbit Aff - Purified Hu WB
50 µg / €440.00
  Novus Biologicals Inc.

ARL3 antibody

Immunohistochemistry-Paraffin: ARL3 Antibody [NBP1-88839] - Immunohistochemical staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ARL3

ARL3 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

ARL3 (1-182, His-tag)

ARL3 Human Purified > 95 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

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