ARMET / ARP antibody

Principal name

ARMET / ARP antibody

Alternative names for ARMET / ARP antibody

Arginine-rich protein, MANF, Mesencephalic astrocyte-derived neurotrophic factor

SwissProt ID

P0C5H9 (Rat), P55145 (Human), P80513 (Bovin), Q9CXI5 (Mouse)

Gene ID

7873 (MANF)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Bov (Bovine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Rabbit, Goat, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection))

Background of ARMET / ARP antibody

MANF, also known as ARMET, was initially identified as a protein containing an arginine-rich region that was highly mutated in a variety of tumors. More recently it was identified as a mesencephalic astrocyte-derived neurotrophic factor with selectivity for dopaminergic neurons, similar to glial cell line-derived neurotrophic factor (GDNF) and CDNF. In rat brain slices, MANF enhanced nigral gamma-aminobutyric acid release. Like GDNF and CDNF, MANF has selective neuroprotective activity for dopaminergic neurons suggesting that it may be indicated for the treatment of Parkinson's disease. Expression of MANF has also been shown to be induced during ER stress, suggesting that it may play a role in protein quality control during ER stress. This antibody does not cross-react with CDNF.

General readings

Shridhar R, Shridhar V, Rivard S, Siegfried JM, Pietraszkiewicz H, Ensley J, Pauley R, Grignon D, Sakr W, Miller OJ, Smith DI.
Mutations in the arginine-rich protein gene, in lung, breast, and prostate cancers, and in squamous cell carcinoma of the head and neck.
Cancer Res. 1996 Dec 15;56(24):5576-8.
PMID: 8971156

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ARMET / ARP antibody

TA302417 (0.3µg/ml) staining of Human Pancreas lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ARMET / ARP (C-term) antibody

Western blot analysis of MANF in mouse brain tissue lysate with MANF antibody at (A) 1 and (B) 2 ug/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ARMET / ARP (N-term) antibody

Western blot analysis of MANF in rat brain tissue lysate with MANF antibody at (A) 1 and (B) 2 ug/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ARMET / ARP antibody

WB Suggested Anti-MANF Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HCT15 cell lysate MANF is supported by BioGPS gene expression data to be expressed in HCT15 Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

ARMET / ARP antibody

WesternBlot testing of anti-MANF monoclonal antibody. Lane 1. Page Ruler prestained protein ladder, Fermentas; Lane 2. Purified recombinant MANF (500 ng per line) expressed using CHOEBNALT85  cell line. Mouse IgG1 1G12 Aff - Purified Hu E, WB
0.1 mg / €400.00
  Acris Antibodies GmbH

ARMET / ARP (C-term) antibody

Human Brain, Cerebellum (formalin-fixed, paraffin-embedded) stained with ARMET antibody AP22598PU-N at 5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Purified Hu, Ms, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

ARMET / ARP Control Peptide

ARMET / ARP
50 µg / €160.00
  Acris Antibodies GmbH

ARMET / ARP Control Peptide

ARMET / ARP
50 µg / €160.00
  Acris Antibodies GmbH

ARMET / ARP antibody

WesternBlot testing of anti-MANF polyclonal antibody. Lane 1: PageRuler Prestained Protein Ladder (#SM0671 Fermentas); Lane 2: Purifiedrecombinant MANF (1 µg per line) expressed using CHOEBNALT85  cell line. Rabbit IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Acris Antibodies GmbH

ARMET / ARP (N-term) antibody

Western blot analysis of ARMET / ARP Antibody (N-term) Cat.-No AP52596PU-N in 293 cell line lysates (35ug/lane). This demonstrates the MANF antibody detected the MANF protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

ARMET / ARP antibody

Western Blot analysis of ARMET expression in transfected 293T cell line (H00007873-T01) by ARMET MaxPab polyclonal antibody.

Lane 1: ARMET transfected lysate(20.02 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

ARMET / ARP antibody

Western Blot analysis of ARMET expression in transfected 293T cell line (H00007873-T01) by ARMET MaxPab polyclonal antibody.

Lane 1: ARMET transfected lysate(20.02 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

ARMET / ARP antibody

ARMET / ARP Mouse IgG1 1D10 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ARMET / ARP antibody

ARMET polyclonal antibody ( Cat # PAB7400 ) ( 0.3 µg/ml ) staining of human pancreas lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

ARMET / ARP antibody

Immunohistochemistry of ARMET in human brain tissue with ARMET polyclonal antibody ( Cat # PAB13301 ) at 2.5 µg/ml. Rabbit Hu, Ms, Rt P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

ARMET / ARP antibody

Immunohistochemistry of ARMET in human brain tissue with ARMET polyclonal antibody ( Cat # PAB13302 ) at 2.5 µg/ml. Rabbit Hu, Ms, Rt P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

ARMET / ARP antibody

NB 100-825 staining (2µg/ml) of HeLa lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

ARMET / ARP antibody

NBP1-06020 (0.3ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

ARMET / ARP antibody

Western Blot: ARMET Antibody  [NBP1-32037]-(30 µg of whole cell lysate) A:293T diluted at 1:1500 Rabbit IgG Hu ICC/IF, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

ARMET / ARP antibody

Immunohistochemistry-Paraffin: ARMET Antibody [NBP1-76836] - Human brain tissue with MANF antibody at 2.5 ug/mL._x000d_ Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

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