Artemis antibody

Principal name

Artemis antibody

Alternative names for Artemis antibody

DCLRE1C, ASCID, SCIDA, SNM1C, SNM1-like protein, A-SCID protein, DNA cross-link repair 1C protein

SwissProt ID

Q5QJC2 (Chick), Q5XIX3 (Rat), Q8K4J0 (Mouse), Q96SD1 (Human)

Gene ID

64421 (DCLRE1C)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Bov (Bovine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Rabbit, Goat

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection))

Background of Artemis antibody

Artemis is a widely expressed 78 kD protein involved in non-homologous end joining. This protein has been reported to be involved in DNA double-strand break repair and the processing of hairpin coding ends during VDJ recombination. Artemis has endonuclease and exonuclease activity and has been shown to be mutated in human severe combined immune deficiency disorder (T-B- radiosensitive SCID). Artemis is phosphorylated by DNA-PKcs; phosphorylation regulates intrinsic endonuclease and exonuclease activity. Artemis has been shown to form a complex with DNA-PKcs.
Structure: Protein involved in non-homologous end joining, predicted molecular weight approximately 78 kD. 
Distribution: Widely expressed. 
Function: Involved in DNA double-strand break repair and the processing of hairpin coding ends during VDJ recombination. Has endonuclease and exonuclease activity. Mutated in human severe combined immune deficiency disorder (T-B- radiosensitive SCID). 
Interaction: Forms a complex with DNA-PKcs.

General readings

1. Pannicke U, Ma Y, Hopfner KP, Niewolik D, Lieber MR, Schwarz K. (2004) Functional and biochemical dissection of the structure-specific nuclease ARTEMIS. EMBO J.;23(9):1987-97.
2. Kobayashi,N. et al. (2003) Novel Artemis gene mutations of radiosensitive severe combined immunodeficiency in Japanese families. Hum.Genet. 112 (4), 348-352.
3. Noordzij,J.G. et al. (2003) Radiosensitive SCID patients with Artemis gene mutations show a complete B-cell differentiation arrest at the pre-Bcell receptor checkpoint in bone marrow. Blood 101 (4), 1446-1452.
4. Moshous,D. et al. (2003) Partial T and B lymphocyte immunodeficiency and predisposition to lymphoma in patients with hypomorphic mutations in Artemis.
J. Clin. Invest. 111 (3), 381-387.
5. Rooney, S. et al. (2003) Defective DNA Repair and Increased Genomic Instability in Artemis-deficient Murine Cells. J. Exp. Med. 197;5: 553-565.
6. Hous D, Callebaut I, de Chasseval R, Corneo B, Cavazzana-Calvo M, Le Deist F, Tezcan I, Sanal O, Bertrand Y, Philippe N, Fischer A, De Villartay JP: (2001) Artemis, a novel DNA double-strand break repair/V(D)J recombination protein, is mutated in human severe combined immune deficiency. Cell 105(2): 177-186.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Artemis antibody

Detection of Artemis protein in human testis lysate using anti-Artemis. Rabbit Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Artemis antibody

Western blotting. Goat-anti-Artemis was used at a 1:500 dilution to detect Artemis is various cell lysates by Western blot. CJ is an Artemis deficient cell line and so no band is visible. Goat Aff - Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Artemis antibody

Western blot analysis of extracts of various cell lines, using DCLRE1C antibody. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Artemis antibody

WB Suggested Anti-DCLRE1C Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: 293T cell lysate DCLRE1C is supported by BioGPS gene expression data to be expressed in HEK293T Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Artemis antibody

WB Suggested Anti-DCLRE1C Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 2500; Positive Control: 293T cell lysate DCLRE1C is supported by BioGPS gene expression data to be expressed in HEK293T Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Artemis antibody

Figure 1. Staining Artemis in Lymph node by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue. Goat Aff - Purified Hu E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Artemis antibody

Immunofluorescent microscope analysis of Hela cells using with anti-Artemis Antibody Cat.-No AP33127PU (red). Alpha-tubulin Antibody Cat.-No AM33090PU (Clone 10D8) has been labeled with green and nuclei were stain with DAPI (blue). Rabbit IgG Aff - Purified Hu ICC/IF, WB
0.2 ml / €360.00
  Acris Antibodies GmbH

Artemis antibody

Immunofluorescent microscope analysis of Hela cells using with anti-Artemis Antibody Cat.-No AP33127PU (red). Alpha-tubulin Antibody Cat.-No AM33090PU (Clone 10D8) has been labeled with green and nuclei were stain with DAPI (blue). Rabbit IgG Aff - Purified Hu ICC/IF, WB
50 µl / €190.00
  Acris Antibodies GmbH

Artemis (N-term) antibody

Formalin fixed, paraffin embedded human lung carcinoma stained with Artemis Antibody (N-term) Cat.-No AP51199PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DCLRE1C Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Artemis antibody

Western Blot analysis of DCLRE1C expression in transfected 293T cell line (H00064421-T02) by DCLRE1C MaxPab polyclonal antibody.

Lane 1: DCLRE1C transfected lysate(50.00 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Artemis antibody

Immunohistochemistry of DCLRE1C polyclonal antibody ( Cat # PAB10023 ) was used at a 1 : 1000 dilution to detect DCLRE1C by immunohistochemistry in human spleen.
Positive staining of T cells and B lymphocytes is observed in thymus, lymph nodes and spleen.
Tissue was formalin-fixed and paraffin embedded.
Personal Communication, Alan Yen, Life Span Biosciences, Seattle, WA. Hu
0.1 mg / €560.00
  Abnova Taiwan Corp.

Artemis pSer516 antibody

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using Artemis (Phospho-Ser516) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P, WB
50 µg / €205.00
  Assay Biotech

Artemis pSer516 antibody

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using Artemis (Phospho-Ser516) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P, WB
0.1 mg / €310.00
  Assay Biotech

Artemis antibody

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Artemis (Ab-516) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, ICC/IF, P
50 µg / €205.00
  Assay Biotech

Artemis antibody

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Artemis (Ab-516) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, ICC/IF, P
0.1 mg / €310.00
  Assay Biotech

Artemis antibody

Human 293T; WB Suggested Anti-DCLRE1C Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: 293T cell lysate. DCLRE1C is supported by BioGPS gene expression data to be expressed in HEK293T.; DCLRE1C antibody - N-terminal region (ARP51164_P050) in Human 293T cells using Western Blot Rabbit Aff - Purified Bov, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

Artemis antibody

Human 293T; WB Suggested Anti-DCLRE1C Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:2500. Positive Control: 293T cell lysate. DCLRE1C is supported by BioGPS gene expression data to be expressed in HEK293T.; DCLRE1C antibody - C-terminal region (ARP51165_P050) in Human 293T cells using Western Blot Rabbit Aff - Purified Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

Artemis pSer516 antibody

Immunohistochemical analysis of Artemis (pS516) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms, Rt P, WB
30 µl / €210.00
  Cohesion Biosciences Ltd.

Artemis pSer516 antibody

Immunohistochemical analysis of Artemis (pS516) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 ml / €330.00
  Cohesion Biosciences Ltd.

Artemis pSer516 antibody

Immunohistochemical analysis of Artemis (pS516) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.2 ml / €530.00
  Cohesion Biosciences Ltd.

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