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Artemis antibody

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Principal name

Artemis antibody

Alternative names for Artemis antibody

DCLRE1C, ASCID, SCIDA, SNM1C, SNM1-like protein, A-SCID protein, DNA cross-link repair 1C protein

SwissProt ID

Q5QJC2 (Chick), Q5XIX3 (Rat), Q8K4J0 (Mouse), Q96SD1 (Human)

Gene ID

64421 (DCLRE1C), 227525 (Dclre1c), 259171

Ncbi ID

NP_001029027.1, 9606, NP_001029029, NP_001029027, AAH22254, NM_022487, NM_001033855, NP_001103684.1, NM_146114, NM_147145, NM_001033858, NM_001033857

Available reactivities

Hu (Human), Can (Canine), Ms (Mouse), Rt (Rat), Bov (Bovine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of Artemis antibody

Artemis is a widely expressed 78 kD protein involved in non-homologous end joining. This protein has been reported to be involved in DNA double-strand break repair and the processing of hairpin coding ends during VDJ recombination. Artemis has endonuclease and exonuclease activity and has been shown to be mutated in human severe combined immune deficiency disorder (T-B- radiosensitive SCID). Artemis is phosphorylated by DNA-PKcs; phosphorylation regulates intrinsic endonuclease and exonuclease activity. Artemis has been shown to form a complex with DNA-PKcs.
Structure: Protein involved in non-homologous end joining, predicted molecular weight approximately 78 kD. 
Distribution: Widely expressed. 
Function: Involved in DNA double-strand break repair and the processing of hairpin coding ends during VDJ recombination. Has endonuclease and exonuclease activity. Mutated in human severe combined immune deficiency disorder (T-B- radiosensitive SCID). 
Interaction: Forms a complex with DNA-PKcs.

General readings

1. Pannicke U, Ma Y, Hopfner KP, Niewolik D, Lieber MR, Schwarz K. (2004) Functional and biochemical dissection of the structure-specific nuclease ARTEMIS. EMBO J.;23(9):1987-97.
2. Kobayashi,N. et al. (2003) Novel Artemis gene mutations of radiosensitive severe combined immunodeficiency in Japanese families. Hum.Genet. 112 (4), 348-352.
3. Noordzij,J.G. et al. (2003) Radiosensitive SCID patients with Artemis gene mutations show a complete B-cell differentiation arrest at the pre-Bcell receptor checkpoint in bone marrow. Blood 101 (4), 1446-1452.
4. Moshous,D. et al. (2003) Partial T and B lymphocyte immunodeficiency and predisposition to lymphoma in patients with hypomorphic mutations in Artemis.
J. Clin. Invest. 111 (3), 381-387.
5. Rooney, S. et al. (2003) Defective DNA Repair and Increased Genomic Instability in Artemis-deficient Murine Cells. J. Exp. Med. 197;5: 553-565.
6. Hous D, Callebaut I, de Chasseval R, Corneo B, Cavazzana-Calvo M, Le Deist F, Tezcan I, Sanal O, Bertrand Y, Philippe N, Fischer A, De Villartay JP: (2001) Artemis, a novel DNA double-strand break repair/V(D)J recombination protein, is mutated in human severe combined immune deficiency. Cell 105(2): 177-186.

AP07832PU-N - Artemis
Figure 1. Staining Artemis in Lymph node by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue.
H00064421-D01P - Artemis
Western Blot analysis of DCLRE1C expression in transfected 293T cell line (H00064421-T02) by DCLRE1C MaxPab polyclonal antibody.Lane 1: DCLRE1C transfected lysate(50.00 KDa).Lane 2: Non-transfected lysate.
PAB10023 - Artemis
Immunohistochemistry of DCLRE1C polyclonal antibody ( Cat # PAB10023 ) was used at a 1 : 1000 dilution to detect DCLRE1C by immunohistochemistry in human spleen.Positive staining of T cells and B lymphocytes is observed in thymus, lymph nodes and spleen.Tissue was formalin-fixed and paraffin embedded.Personal Communication, Alan Yen, Life Span Biosciences, Seattle, WA.
PAB10023 - Artemis
Immunoblotting of DCLRE1C polyclonal antibody ( Cat # PAB10023 ) was used at a 1 : 500 dilution to detect DCLRE1C is various cell lysates by Western blot.CJ is an DCLRE1C deficient cell lineand so no band is visible.This antibody detects a band of approximately 90 kDa ( predicted molecular weight: 78 kDa ).
ARP51165_P050 - Artemis
Human 293T; WB Suggested Anti-DCLRE1C Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:2500. Positive Control: 293T cell lysate. DCLRE1C is supported by BioGPS gene expression data to be expressed in HEK293T.; DCLRE1C antibody - C-terminal region (ARP51165_P050) in Human 293T cells using Western Blot
ARP51164_P050 - Artemis
Human 293T; WB Suggested Anti-DCLRE1C Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: 293T cell lysate. DCLRE1C is supported by BioGPS gene expression data to be expressed in HEK293T.; DCLRE1C antibody - N-terminal region (ARP51164_P050) in Human 293T cells using Western Blot
A0456-1 - Artemis
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using Artemis (Phospho-Ser516) Antibody. The picture on the right is treated with the synthesized peptide.
A0456-1 - Artemis
Western blot analysis of extracts from HepG2 cells treated with EGF 200ng/ml 30', using Artemis (Phospho-Ser516) Antibody. The lane on the right is treated with the synthesized peptide.
B0456-1 - Artemis
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Artemis (Ab-516) Antibody. The picture on the right is treated with the synthesized peptide.
B0456-1 - Artemis
Immunofluorescence analysis of NIH-3T3 cells, using Artemis (Ab-516) Antibody. The picture on the right is treated with the synthesized peptide.
AP51199PU-N - Artemis
Formalin fixed, paraffin embedded human lung carcinoma stained with Artemis Antibody (N-term) Cat.-No AP51199PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DCLRE1C Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
AP51199PU-N - Artemis
Western blot analysis of Artemis Antibody (N-term) Cat.-No AP51199PU-N in Mouse heart tissue lysates (35ug/lane). DCLRE1C (arrow) was detected using the purified Pab.
AP33127PU-S - Artemis
Immunofluorescent microscope analysis of Hela cells using with anti-Artemis Antibody Cat.-No AP33127PU (red). Alpha-tubulin Antibody Cat.-No AM33090PU (Clone 10D8) has been labeled with green and nuclei were stain with DAPI (blue).
AP33127PU-S - Artemis
MOLT-4 cell nuclear extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-Artemis Antibody Cat.-No AP33127PU. Proteins were visualized using a donkey anti-rabbit-IgG secondary conjugated to HRP and chemiluminescence detection.
CPA2758-100ul - Artemis
Immunohistochemical analysis of Artemis (pS516) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
CPA2758-100ul - Artemis
Western blot analysis of Artemis (pS516) expression in HepG2 (A); HeLa (B); mouse skeletal muscle (C); rat skeletal muscle (D) whole cell lysates.
TA309620 - Artemis
Detection of Artemis protein in human testis lysate using anti-Artemis.
TA319208 - Artemis
Western blotting. Goat-anti-Artemis was used at a 1:500 dilution to detect Artemis is various cell lysates by Western blot. CJ is an Artemis deficient cell line and so no band is visible.
TA319208 - Artemis
Affinity Purified anti-Artemis antibody was used at a 1:1000 dilution to detect Artemis by immunohistochemistry in human spleen. Positive staining of T cells and B lymphocytes is observed in thymus, lymph nodes and spleen. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Alan Yen, LifeSpanBiosciences, Seattle, WA.
TA332790 - Artemis
Western blot analysis of extracts of various cell lines, using DCLRE1C antibody.
TA332790 - Artemis
Immunofluorescence analysis of U20S cell using DCLRE1C antibody. Blue: DAPI for nuclear staining.
TA339872 - Artemis
WB Suggested Anti-DCLRE1C Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: 293T cell lysate DCLRE1C is supported by BioGPS gene expression data to be expressed in HEK293T
TA339873 - Artemis
WB Suggested Anti-DCLRE1C Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 2500; Positive Control: 293T cell lysate DCLRE1C is supported by BioGPS gene expression data to be expressed in HEK293T

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Artemis antibody
  Rabbit Polyclonal Artemis Antibody  
Detection of Artemis protein in human testis lysate using anti-Artemis. Rabbit Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Artemis antibody
  Goat polyclonal anti-Artemis antibody  
Western blotting. Goat-anti-Artemis was used at a 1:500 dilution to detect Artemis is various cell lysates by Western blot. CJ is an Artemis deficient cell line and so no band is visible. Goat Aff - Purified Hu E, P, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.
+1 additional image

Artemis antibody
  Rabbit anti-DCLRE1C Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using DCLRE1C antibody. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.
+1 additional image

Artemis antibody
  Rabbit Polyclonal Anti-DCLRE1C Antibody  
WB Suggested Anti-DCLRE1C Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: 293T cell lysate DCLRE1C is supported by BioGPS gene expression data to be expressed in HEK293T Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Artemis antibody
  Rabbit Polyclonal Anti-DCLRE1C Antibody  
WB Suggested Anti-DCLRE1C Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 2500; Positive Control: 293T cell lysate DCLRE1C is supported by BioGPS gene expression data to be expressed in HEK293T Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Artemis antibody
Figure 1. Staining Artemis in Lymph node by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue. Goat Aff - Purified Hu E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Artemis antibody
Immunofluorescent microscope analysis of Hela cells using with anti-Artemis Antibody Cat.-No AP33127PU (red). Alpha-tubulin Antibody Cat.-No AM33090PU (Clone 10D8) has been labeled with green and nuclei were stain with DAPI (blue). Rabbit IgG Aff - Purified Hu ICC/IF, WB
0.2 ml / €360.00
  Acris Antibodies GmbH
+1 additional image

Artemis (N-term) antibody
Formalin fixed, paraffin embedded human lung carcinoma stained with Artemis Antibody (N-term) Cat.-No AP51199PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DCLRE1C Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH
+1 additional image

Artemis pSer516 antibody
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using Artemis (Phospho-Ser516) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P, WB
50 µg / €230.00
  Assay Biotech
+1 additional image

Artemis pSer516 antibody
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using Artemis (Phospho-Ser516) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P, WB
0.1 mg / €330.00
  Assay Biotech
+1 additional image

Artemis antibody
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Artemis (Ab-516) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, ICC/IF, P
50 µg / €230.00
  Assay Biotech
+1 additional image

Artemis antibody
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Artemis (Ab-516) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, ICC/IF, P
0.1 mg / €330.00
  Assay Biotech
+1 additional image

CRISPR

Catalog No. Species Donor Vector  

DCLRE1C - human gene knockout kit via CRISPR
DCLRE1C - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

DCLRE1C - human gene knockout kit via CRISPR
DCLRE1C - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

DCLRE1C - human gene knockout kit via CRISPR
DCLRE1C - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

DCLRE1C - human gene knockout kit via CRISPR
DCLRE1C - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Dclre1c - mouse gene knockout kit via CRISPR
Dclre1c - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Dclre1c - mouse gene knockout kit via CRISPR
Dclre1c - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Dclre1c - mouse gene knockout kit via CRISPR
Dclre1c - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

DCLRE1C overexpression lysate
DCLRE1C overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

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