ASAP1 / DDEF1 antibody

Principal name

ASAP1 / DDEF1 antibody

Alternative names for ASAP1 / DDEF1 antibody

KIAA1249, PAG2, AMAP1, ZG14P, CENTB4, Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1, 130 kDa phosphatidylinositol 4,5-biphosphate-dependent ARF1 GTPase-activating protein, PIP2-dependent ARF1 GAP, ADP-ribosylation factor-directed GTPase-activating protein 1, Development and differentiation-enhancing factor 1

SwissProt ID

O97902 (Bovin), Q1AAU6 (Rat), Q9QWY8 (Mouse), Q9ULH1 (Human)

Gene ID

50807 (ASAP1), 13196 (Asap1), 314961

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Bov (Bovine), Chimp (Chimpanzee), Chk (Chicken), Can (Canine)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Immunoprecipitation (IP)

Background of ASAP1 / DDEF1 antibody

ASAP1 (also known as AMAP1, 130-kDa phosphatidylinositol 4,5-biphosphate-dependent ARF1 GTPase-activating protein, PIP2-dependent ARF1 GAP,ADP-ribosylation factor-directed GTPase-activating protein 1, ARF GTPase-activating protein 1, Development and differentiation-enhancing factor 1, Differentiation-enhancing factor 1, DEF-1) is an Arf-directed GTPase activating protein that is a substrate for the kinases Src and FAK and has been implicated in the regulation of membrane traffic, focal adhesions and invadopodia/podosomes. Phosphorylation of ASAP1 at tyrosine 782 has been found to affect enzymatic and some biological activities, including the function of invadopodia. ASAP1 is expressed in many tissues but is most abundant in the testis, brain, lung and spleen. A heightened expression was seen in the adipose tissue from obese (ob) and diabetic (db) animals. Multiple transcript variants have been reported for this protein.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ASAP1 / DDEF1 antibody

Immunofluorescence of monoclonal antibody to DDEF1 on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG2a 2G7 Purified Hu, Rt E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ASAP1 / DDEF1 (777-787) antibody

  Rabbit polyclonal anti-ASAP1 antibody  
Immunofluorescent microscopy using Affinity Purified anti-ASAP1 antibody shows detection of ASAP1present in mouse NIH3T3 cells transfected with activated Src. Specific staining is not present when antibody is pre-incubated with the immunizing peptide prior to reaction with cells. Personal Communication. Paul Randazzo, NIH, CCR, Bethesda, MD. Rabbit IgG Aff - Purified Bov, Can, Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ASAP1 / DDEF1 antibody

  Rabbit polyclonal ASAP1 phospho Y782 antibody (Phospho-specific)  
ASAP1 / DDEF1 Rabbit IgG Purified Bov, Can, Chimp, Chk, Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ASAP1 / DDEF1 antibody

  Mouse monoclonal Anti-ASAP1 Clone 7B12  
ASAP1 / DDEF1 Mouse IgG2a 7B12 Purified Hu, Rt E, IP, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ASAP1 / DDEF1 antibody

ASAP1 / DDEF1 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

ASAP1 / DDEF1 antibody

DDEF1 monoclonal antibody (M02), clone 1B5. Western Blot analysis of DDEF1 expression in NIH/3T3 ( Cat # L018V1 ). Mouse IgG2a 1B5 Purified Hu, Ms E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ASAP1 / DDEF1 pTyr782 antibody

Asap1 (phospho Y782) polyclonal antibody ( Cat # PAB10087 ) was used at 20 µg/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides.
This image shows moderate intracellular positive staining in epidermal keratinocytes in human skin at 40X.
Tissue was formalin-fixed and paraffin embedded.
The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
Personal Communi-cation, Tina Roush, Life Span Biosciences, Seattle, WA. Rabbit Bov, Chimp, Chk, Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

ASAP1 / DDEF1 antibody

Immunofluorescent microscopy using Asap1 polyclonal antibody ( Cat # PAB10088 ) shows detection of Asap1 present in mouse NIH/3T3 cells transfected with activated Src.
Specific staining is not present when antibody is pre-incubated with the immunizing peptide prior to reaction with cells.
Personal Communication. Paul Randazzo, NIH, CCR, Bethesda, MD. Rabbit Bov, Chimp, Chk, Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

ASAP1 / DDEF1 antibody

ASAP1 / DDEF1 Rabbit IgG Aff - Purified Hu, Ms C, WB
50 µg / €680.00
  Funakoshi Co., Ltd.

CRISPR

Catalog No. Species Donor Vector  

ASAP1 - human gene knockout kit via CRISPR

ASAP1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

ASAP1 - human gene knockout kit via CRISPR

ASAP1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

ASAP1 - human gene knockout kit via CRISPR

ASAP1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

ASAP1 - human gene knockout kit via CRISPR

ASAP1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Asap1 - mouse gene knockout kit via CRISPR

Asap1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Asap1 - mouse gene knockout kit via CRISPR

Asap1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Asap1 - mouse gene knockout kit via CRISPR

Asap1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Asap1 - mouse gene knockout kit via CRISPR

Asap1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ASAP1 / DDEF1

ASAP1 / DDEF1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

ASAP1 / DDEF1

ASAP1 / DDEF1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, ASAP1 (mGFP-tagged) - Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (ASAP1), 200 uL, >10^7 TU/mL

Lenti ORF particles, ASAP1 (mGFP-tagged) - Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (ASAP1), 200 uL, >10^7 TU/mL  
200 µl / €1,320.00
  OriGene Technologies, Inc.

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