ASAP1 / DDEF1 antibody

Principal name

ASAP1 / DDEF1 antibody

Alternative names for ASAP1 / DDEF1 antibody

KIAA1249, PAG2, AMAP1, ZG14P, CENTB4, Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1, 130 kDa phosphatidylinositol 4,5-biphosphate-dependent ARF1 GTPase-activating protein, PIP2-dependent ARF1 GAP, ADP-ribosylation factor-directed GTPase-activating protein 1, Development and differentiation-enhancing factor 1

SwissProt ID

O97902 (Bovin), Q1AAU6 (Rat), Q9QWY8 (Mouse), Q9ULH1 (Human)

Gene ID

50807 (ASAP1), 13196

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Bov (Bovine), Chimp (Chimpanzee), Chk (Chicken), Can (Canine), Mky (Monkey)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), Frozen Sections (C)

Background of ASAP1 / DDEF1 antibody

ADP-ribosylation factors (ARFs) family of GTP binding proteins are involved in the regulation of membrane trafficking and actin cytoskeleton. ASAP1 (ARF GAP containing SH3, ANK repeat and PH domains 1, also known as DEF1 or centaurin beta 4) is a 130 kDa, ADP ribosylation factor GTPase activating protein (ARFGAP). ARFs require ARF-GAPs that stimulate their GTPase activity. ASAP1 belongs to a family of related ARF-GAP proteins, consisting of PAP/DEF2, ACAP1, ACAP2, GIT1/CAT1/APP1, and GIT2/CAT2/PKL, which are involved in the regulation of the actin cytoskeleton and cell migration. ASAP1 binds ARF1 and ARF5, with only modest activity toward ARF6. It also binds to other known regulators of actin cytoskeleton, such as tyrosine kinase Src, Crk, FAK and phosphatidylinositol-4,5-bisphosphate (PIP2). ASAP1 is phosphorylated on tyrosine residues in cells expressing activated Src.ASAP1a and ASAP1b are two variants formed by alternative splicing of the ASAP1 gene. ASAP1 localizes to focal adhesions and cycles with focal adhesion proteins when cells are stimulated to move. Overexpression of ASAP1 alters the morphology of focal adhesions, blocks cell spreading and formation of dorsal ruffles, and prevents the efficient organization of paxillin and FAK in focal adhesions. On the other hand, a mutation disrupting GAP activity of ASAP1 or overexpression of a truncated variant of ASAP1 that fails to bind FAK has a reduced effect on inhibition of cell spreading. By directly interacting with both ARFs and focal adhesion proteins such as paxillin and FAK, ASAP1 plays an important role in the regulation of focal adhesion assembly and cytoskeletal remodeling.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ASAP1 / DDEF1 antibody

Immunofluorescence of monoclonal antibody to DDEF1 on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG2a 2G7 Purified Hu, Rt E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ASAP1 / DDEF1 (777-787) antibody

Immunofluorescent microscopy using Affinity Purified anti-ASAP1 antibody shows detection of ASAP1present in mouse NIH3T3 cells transfected with activated Src. Specific staining is not present when antibody is pre-incubated with the immunizing peptide prior to reaction with cells. Personal Communication. Paul Randazzo, NIH, CCR, Bethesda, MD. Rabbit IgG Aff - Purified Bov, Can, Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ASAP1 / DDEF1 pTyr782 antibody

Immunohistochemistry. Affinity purified anti-ASAP1 pY782 antibody was used at 20 µg/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate intracellular positive staining in epidermal keratinocytes in human skin at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Rabbit IgG Aff - Purified Bov, Can, Hu, Ms, Rt E, P, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

ASAP1 / DDEF1 antibody

ASAP1 / DDEF1 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

ASAP1 / DDEF1 antibody

DDEF1 monoclonal antibody (M02), clone 1B5. Western Blot analysis of DDEF1 expression in NIH/3T3 ( Cat # L018V1 ). Mouse IgG2a 1B5 Purified Hu, Ms E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ASAP1 / DDEF1 pTyr782 antibody

Asap1 (phospho Y782) polyclonal antibody ( Cat # PAB10087 ) was used at 20 µg/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides.
This image shows moderate intracellular positive staining in epidermal keratinocytes in human skin at 40X.
Tissue was formalin-fixed and paraffin embedded.
The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
Personal Communi-cation, Tina Roush, Life Span Biosciences, Seattle, WA. Rabbit Bov, Chimp, Chk, Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

ASAP1 / DDEF1 antibody

Immunofluorescent microscopy using Asap1 polyclonal antibody ( Cat # PAB10088 ) shows detection of Asap1 present in mouse NIH/3T3 cells transfected with activated Src.
Specific staining is not present when antibody is pre-incubated with the immunizing peptide prior to reaction with cells.
Personal Communication. Paul Randazzo, NIH, CCR, Bethesda, MD. Rabbit Bov, Chimp, Chk, Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

ASAP1 / DDEF1 antibody

ASAP1 / DDEF1 Rabbit IgG Aff - Purified Hu, Ms C, WB
50 µg / €680.00
  Funakoshi Co., Ltd.

ASAP1 / DDEF1 antibody

Western Blot and Immunoprecipitation: ASAP1 Antibody [NBP1-19158] - Whole cell lysate from HeLa cells used for WB. ASAP1 was also immunoprecipitated by rabbit anti-ASAP1 antibody NBP1-19159. Rabbit IgG Aff - Purified Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

ASAP1 / DDEF1 antibody

Western Blot: ASAP1 Antibody [NBP1-19159] - ASAP1 by Western Blot and Immunoprecipitation: ASAP1 Antibody [NBP1-19159]- Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. ASAP1 was also immunoprecipitated by rabbit anti-ASAP1 antibody NBP1-19158, which recognizes an upstream epitope. Rabbit IgG Aff - Purified Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

ASAP1 / DDEF1 antibody

Immunofluorescence: ASAP1 Antibody [NBP1-77934] Affinity Purified anti-ASAP1 antibody shows detection of ASAP1present in mouse NIH3T3 cells transfected with activated Src. Specific staining is not present when antibody is pre-incubated with the immunizing peptide prior to reaction with cells. Rabbit Aff - Purified Bov, Can, Chk, Hu, Mky, Rt E, P, WB
0.1 mg / €510.00
  Novus Biologicals Inc.

ASAP1 / DDEF1 antibody

Western Blot: ANKRD26 Antibody [NBP1-77935] shows detection of a band at ~81 kDa corresponding to mouse Ankrd26 protein. Lane 1 Blank, Lane 2 MES cell lysate - 80 ug, Lane 3 MES cell lysate - 40 ug, Lane 4 293T-ANKRD26 transfected cell lysate - 20 ug, Lane 5 control 293T cell lysate - 20 µg, Lane 6 BSA-ANKRD26 conjugate 20 ng, Lane 7 BSA - 500 ng, Lane 8 BSA - 100 ng, Lane 9 BSA 20 ng and Lane 10 Protein standards. Detection of endogenous Ankrd26 protein in MES cell lysates may occur when detection methods with higher sensitivity are used. Proteins were separated by SDS-PAGE, transferred to nitrocellulose, and probed with the primary antibody diluted to 1:1,000 followed by detection using ALP conjugated Gt-a-Rabbit IgG diluted to 1:3,000. Size estimation was made by comparison to prestained MW markers as indicated. Rabbit Aff - Purified Ms E, WB
0.1 mg / €510.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ASAP1 / DDEF1

ASAP1 / DDEF1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

ASAP1 / DDEF1

ASAP1 / DDEF1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

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