ASH2L / ASH2L1 antibody

Principal name

ASH2L / ASH2L1 antibody

Alternative names for ASH2L / ASH2L1 antibody

ASH2-like protein, Set1/Ash2 histone methyltransferase complex subunit ASH2

SwissProt ID

Q91X20 (Mouse), Q9UBL3 (Human)

Gene ID

9070 (ASH2L), 23808 (Ash2l)

Available reactivities

Bov (Bovine), Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Chk (Chicken), Mky (Monkey), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Mouse, Rat

Available applications

Western blot / Immunoblot (WB), ChIP assay (ChIP), Paraffin Sections (P), Immunoprecipitation (IP), PLA/Proximity ligation assay (PLA), Enzyme Immunoassay (E), Frozen Sections (C)

Background of ASH2L / ASH2L1 antibody

We know how important it is to your research to have access to antibodies specific for your model. As it is often impossible to know which antibody will work best for a specific sample and application, we now offer SuperNovus Packs. These packs include different antibodies to the same protein to give you the ability to decide for yourself. The SuperNovus Packs are all offered at a discount price compared to purchasing each antibody individually. The ash2 gene is a member of the trithorax group of genes whose products function to maintain active transcription of homeotic selector genes. Mutations in ash2 cause the homeotic transformations expected for a gene in this group but, in addition, cause a variety of pattern formation defects that are not necessarily expected. The ash2 gene is located in cytogenetic region 96A17-19 flanked by slowpoke and tolloid and is included in a cosmid that contains part of slowpoke. Ash2, the product of the Drosophila trxG gene absent, small, or homeotic discs 2 (ash2) is a component of a 500-kD complex. ASH2 binds directly to Skittles (Sktl), a predicted phosphatidylinositol 4-phosphate 5-kinase, and the association of these proteins is functionally significant. Histone H1 hyperphosphorylation is dramatically increased in both ash2 and sktl mutant polytene chromosomes. These results suggest that Ash2 maintains active transcription by binding a producer of nuclear phosphoinositides and downregulating histone H1 hyperphosphorylation. Both Ash2 and Sktl accumulate on polytene chromosomes and in the nucleolus. These results suggest that their physical association and functions involve chromatin and, perhaps, ribosomal DNA transcription. Ash2 accumulates normally on sktl mutant chromosomes while Sktl does not accumulate on ash2 mutant chromosomes.

General readings

Yokoyama,A., et al., (2004) Mol. Cell. Biol. 24 (13), 5639-5649

  1. Quick-Filters:
  2. Highly Validated (1)

Items 1 to 20 of 27 total

per page
Page:
  1. 1
  2. 2

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ASH2L / ASH2L1 Super Novus Pack antibody

Western Blot and  Immunoprecipitation: ASH2 Antibodies SuperNovus Pack [NB100-942] - Detection of Human ASH2 on HeLa whole cell lysate using NB600-281. ASH2 was also immunoprecipitated with rabbit anti-ASH2 antibody NB600-250. Rabbit Aff - Purified Hu WB
4 vials / €930.00
  Novus Biologicals Inc.

ASH2L / ASH2L1 antibody

Western blot analysis of extracts of various cell lines, using ASH2L antibody. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ASH2L / ASH2L1 antibody

WB Suggested Anti-ASH2L Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Hela cell lysate Rabbit IgG Purified Can, Eq, GP, Hu, Ms, Rb, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

ASH2L / ASH2L1 antibody

WB Suggested Anti-ASH2L Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Jurkat cell lysate; ASH2L is supported by BioGPS gene expression data to be expressed in Jurkat Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

ASH2L / ASH2L1 antibody

WB Suggested Anti-ASH2L Antibody; Titration: 2.5 ug/ml; Positive Control: Jurkat Whole Cell; ASH2L is supported by BioGPS gene expression data to be expressed in Jurkat Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ASH2L / ASH2L1 antibody

WB Suggested Anti-ASH2L Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Hela cell lysate Rabbit IgG Purified Can, Eq, Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

ASH2L / ASH2L1 antibody

WB was performed on whole cell lysates from mouse fibroblastst (NIH3T3) and embryonic stem cells (E14Tg2a) with the ab at 1:1,000 (predicted size: 68 kDa). B. WB was performed on whole cell lysates from mouse neural stem cells (NS), transfected with GFP tagged Ash2, with the ab against mouse Ash2, diluted 1:500 in BSA/PBS-Tween. The molecular weight marker (in kDa) is shown on the left; the location of the endogenous Ash2 (68 kDa) and of the GFP tagged Ash2 (106 kDa) are indicated on the right. Rabbit IgG Purified Ms E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ASH2L / ASH2L1 antibody

WB was performed using the hASH2 ab at 1:1,000 in TBS-Tween with 5% skimmed milk on nuclear extracts from HeLa cells (upper panel: lane 1) and ectopically expressed FLAG-tagged ASH2 (ASH2-F, upper panel: lane 2). The lower panel shows the same WB was performed using nuclear extracts from HeLa (HeLa NE, 20 ug) with the ASH2 ab at 1:1,000 in TBS-Tween with 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right. Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ASH2L / ASH2L1 antibody

WB was performed on HeLa nuclear extract (HeLa NE, 20 ug) using the antibody against ASH2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right. Rabbit IgG Purified Hu
50 µg / €325.00
  OriGene Technologies, Inc.

ASH2L / ASH2L1 antibody

ASH2L / ASH2L1 Mouse IgG2a 4G7 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ASH2L / ASH2L1 antibody

ASH2L monoclonal antibody (M21), clone 1B4. Western Blot analysis of ASH2L expression in Jurkat(Cat # L017V1 ). Mouse IgG2b 1B4 Ascites Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

ASH2L / ASH2L1 antibody

ASH2L / ASH2L1 Rabbit Hu IP, WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

ASH2L / ASH2L1 antibody

Western blot was performed on whole cell lysates from mouse fibroblastst ( Lane 1, NIH/3T3 ) and embryonic stem cells ( Lane 2, E14Tg2a ) with Ash2l polyclonal antibody ( Cat # PAB14060 ), diluted 1 : 1,000 in BSA/PBS-Tween. Rabbit Ms E, WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

ASH2L / ASH2L1 antibody

Western blot was performed on HeLa nuclear extract ( HeLa NE, 20 µg ) using ASH2L polyclonal antibody ( Cat # PAB14106 ) at dilution 1 : 1,000 in TBS-Tween + 5% skimmed milk.
The antibody recognizes endogenous human ASH2 in HeLa nuclear extract.
On the left side, a molecular weight marker is shown.
The arrow indicates the location of the protein of interest. Rabbit Hu WB
50 µg / €390.00
  Abnova Taiwan Corp.

ASH2L / ASH2L1 antibody

Human Placenta; Immunohistochemistry with Human Placenta lysate tissue at an antibody concentration of 5.0ug/ml using anti-ASH2L antibody (ARP34202_P050); ASH2L antibody - middle region (ARP34202_P050) in Human Placenta cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

ASH2L / ASH2L1 antibody

Human Jurkat; WB Suggested Anti-ASH2L Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate. ASH2L is supported by BioGPS gene expression data to be expressed in Jurkat.; ASH2L antibody - N-terminal region (ARP34203_P050) in Human Jurkat cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

ASH2L / ASH2L1 antibody

Human Heart; . Rabbit Anti-ASH2L antibody. . Catalog Number:. ARP34203_T100 . Paraffin Embedded Tissue: Human Heart cell . Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; ASH2L antibody (ARP34203_T100) in Human Heart cells using Immunohistochemistry Rabbit Aff - Purified Can, Hu, Rt WB
0.1 mg / €310.00
  AVIVA Systems Biology

ASH2L / ASH2L1 antibody

Human HeLa; WB Suggested Anti-ASH2L Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Hela cell lysate; ASH2L antibody - N-terminal region (ARP58134_P050) in Human HeLa cells using Western Blot Rabbit Aff - Purified Can, Hu WB
50 µg / €350.00
  AVIVA Systems Biology

ASH2L / ASH2L1 antibody

WB and IP: ASH2 Antibody [NB600-250] - Detection of Human ASH2 on HeLa whole cell lysates using NB600-250. Immunoprecipitated ASH2 was detected by WB using affinity purified rabbit anti-ASH2 antibody NB600-251 used. Rabbit Aff - Purified Hu ChIP, IP, P, PLA, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

ASH2L / ASH2L1 antibody

Western Blot: ASH2 Antibody [NB600-253] - Detection of Human ASH2 by Western Blot. Samples: Whole cell lysate (A. 40 ug E; 10 ug  T) from 293T cells that were mock transfected (E) or transfected with an ASH2 expression construct (T). Antibody: NB600-253 used at the indicated concentrations. Detection: Chemiluminescence with a 10 second exposure. Rabbit Aff - Purified Hu, Ms P, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

Items 1 to 20 of 27 total

per page
Page:
  1. 1
  2. 2
  • LinkedIn