ASPH antibody

Principal name

ASPH antibody

Alternative names for ASPH antibody

ASP beta-hydroxylase, HAAH, JCTN, junctin, Aspartyl/asparaginyl beta-hydroxylase, Aspartate beta-hydroxylase, Peptide-aspartate beta-dioxygenase

SwissProt ID

Q12797 (Human), Q28056 (Bovin), Q8BSY0 (Mouse)

Gene ID

444 (ASPH)

Available reactivities

Bov (Bovine), Chk (Chicken), Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Rb (Rabbit), Eq (Equine), Por (Porcine), GP (Guinea Pig)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Enzyme Immunoassay (E), Paraffin Sections (P), Immunoprecipitation (IP), Frozen Sections (C)

Background of ASPH antibody

This gene is thought to play an important role in calcium homeostasis. The gene is expressed from two promoters and undergoes extensive alternative splicing. The encoded set of proteins share varying amounts of overlap near their N-termini but have substantial variations in their C-terminal domains resulting in distinct functional properties. The longest isoforms (a and f) include a C-terminal Aspartyl/Asparaginyl beta-hydroxylase domain that hydroxylates aspartic acid or asparagine residues in the epidermal growth factor (EGF)-like domains of some proteins, including protein C, coagulation factors VII, IX, and X, and the complement factors C1R and C1S. Other isoforms differ primarily in the C-terminal sequence and lack the hydroxylase domain, and some have been localized to the endoplasmic and sarcoplasmic reticulum. Some of these isoforms are found in complexes with calsequestrin, triadin, and the ryanodine receptor, and have been shown to regulate calcium release from the sarcoplasmic reticulum. Some isoforms have been implicated in metastasis.

General readings

Kwon SJ., et al. (2009) Biochem Biophys Res Commun. 390(4):1389-94.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ASPH antibody

WB Suggested Anti-ASPH Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: MCF7 cell lysate Rabbit IgG Purified Can, Eq, Hu, Por, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

ASPH antibody

WB Suggested Anti-ASPH Antibody Titration: 5.0 ug/ml; ELISA Titer: 1:312500; Positive Control: Jurkat cell lysate Rabbit IgG Purified Can, Eq, GP, Hu, Ms, Por, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ASPH antibody

WB Suggested Anti-ASPH Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: ACHN cell lysate Rabbit IgG Purified Bov, Hu, Ms, Por, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

ASPH antibody

1Human 721_B; Host: Rabbit; Target Name: ASPH; Sample Tissue: 721_B; Antibody Dilution: 1.0 ug/ml ASPH is supported by BioGPS gene expression data to be expressed in 721_B Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

ASPH antibody

WB Suggested Anti-ASPH Antibody Titration: 2.5 ug/ml; Positive Control: HepG2 cell lysateThere is BioGPS gene expression data showing that ASPH is expressed in HepG2 Rabbit IgG Purified Can, Eq, Hu, Ms, Por, Rb, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ASPH antibody

WB Suggested Anti-ASPH Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Human Stomach Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

ASPH (Center) antibody

ASPH Antibody (Center) (Cat. #AP50276PU-N)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ASPH Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

ASPH antibody

Western Blot analysis of ASPH expression in transfected 293T cell line (H00000444-T01) by ASPH MaxPab polyclonal antibody.

Lane 1: ASPH transfected lysate(29.81 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

ASPH antibody

Western Blot analysis of ASPH expression in transfected 293T cell line (H00000444-T01) by ASPH MaxPab polyclonal antibody.

Lane 1: ASPH transfected lysate(29.81 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

ASPH antibody

Western Blot analysis of ASPH expression in transfected 293T cell line (H00000444-T02) by ASPH MaxPab polyclonal antibody.

Lane 1: ASPH transfected lysate(23.1 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

ASPH antibody

Western Blot analysis of ASPH expression in transfected 293T cell line (H00000444-T04) by ASPH MaxPab polyclonal antibody.

Lane 1: ASPH transfected lysate(29.81 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

ASPH antibody

Immunoprecipitation of ASPH transfected lysate using anti-ASPH MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with ASPH purified MaxPab mouse polyclonal antibody (B02P) (H00000444-B02P). Rabbit Serum Hu E(detection), IP
0.1 ml / €380.00
  Abnova Taiwan Corp.

ASPH antibody

Western Blot analysis of ASPH expression in transfected 293T cell line (H00000444-T03) by ASPH MaxPab polyclonal antibody.

Lane 1: ASPH transfected lysate(23.80 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

ASPH antibody

Detection limit for recombinant GST tagged ASPH is approximately 1ng/ml as a capture antibody. Mouse IgG2a 3G5 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ASPH antibody

ASPH Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

ASPH antibody

Human Jurkat; WB Suggested Anti-ASPH Antibody Titration: 5.0ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate; ASPH antibody - middle region (ARP34195_T100) in Human Jurkat cells using Western Blot Rabbit Aff - Purified Bov, Chk, Hu, Ms, Rt WB
0.1 mg / €310.00
  AVIVA Systems Biology

ASPH antibody

Human Small intestine; Immunohistochemistry with Small intestine, myenteric plexus tissue at an antibody concentration of 5ug/ml using anti-ASPH antibody (ARP34691_P050); ASPH antibody - middle region (ARP34691_P050) in Human Small intestine cells using Immunohistochemistry Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

ASPH antibody

Human 721_B; Host: Rabbit. Target Name: WT1 . Sample Tissue: 721_B . Antibody Dilution: 1.0ug/ml. ASPH is supported by BioGPS gene expression data to be expressed in 721_B.; ASPH antibody - N-terminal region (ARP51356_P050) in Human 721_B cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

ASPH antibody

Human kidney; ASPH antibody - N-terminal region (ARP51357_T100) in Human kidney cells using Immunohistochemistry Rabbit Aff - Purified Can, Hu, Ms, Rb, Rt P, WB
0.1 mg / €310.00
  AVIVA Systems Biology

ASPH antibody

Western Blot: Aspartate beta hydroxylase Antibody [NBP1-69229] - This Anti-ASPH antibody was used in Western Blot of HepG2 tissue lysate at a concentration of 0.5ug/ml. Rabbit Aff - Purified Hu WB
50 µg / €440.00
  Novus Biologicals Inc.

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