ASPH antibody

Principal name

ASPH antibody

Alternative names for ASPH antibody

ASP beta-hydroxylase, HAAH, JCTN, junctin, Aspartyl/asparaginyl beta-hydroxylase, Aspartate beta-hydroxylase, Peptide-aspartate beta-dioxygenase

SwissProt ID

Q12797 (Human), Q28056 (Bovin), Q8BSY0 (Mouse)

Gene ID

444 (ASPH), 65973 (Asph)

Available reactivities

Bov (Bovine), Chk (Chicken), Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Rb (Rabbit), Eq (Equine), Por (Porcine), GP (Guinea Pig)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Enzyme Immunoassay (E), Paraffin Sections (P), Immunoprecipitation (IP)

Background of ASPH antibody

This gene is thought to play an important role in calcium homeostasis. The gene is expressed from two promoters and undergoes extensive alternative splicing. The encoded set of proteins share varying amounts of overlap near their N-termini but have substantial variations in their C-terminal domains resulting in distinct functional properties. The longest isoforms (a and f) include a C-terminal Aspartyl/Asparaginyl beta-hydroxylase domain that hydroxylates aspartic acid or asparagine residues in the epidermal growth factor (EGF)-like domains of some proteins, including protein C, coagulation factors VII, IX, and X, and the complement factors C1R and C1S. Other isoforms differ primarily in the C-terminal sequence and lack the hydroxylase domain, and some have been localized to the endoplasmic and sarcoplasmic reticulum. Some of these isoforms are found in complexes with calsequestrin, triadin, and the ryanodine receptor, and have been shown to regulate calcium release from the sarcoplasmic reticulum. Some isoforms have been implicated in metastasis.

General readings

Kwon SJ., et al. (2009) Biochem Biophys Res Commun. 390(4):1389-94.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ASPH antibody

  Rabbit Polyclonal Anti-ASPH Antibody  
WB Suggested Anti-ASPH Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: MCF7 cell lysate Rabbit IgG Purified Can, Eq, Hu, Por, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

ASPH antibody

  Rabbit Polyclonal Anti-ASPH Antibody  
WB Suggested Anti-ASPH Antibody Titration: 5.0 ug/ml; ELISA Titer: 1:312500; Positive Control: Jurkat cell lysate Rabbit IgG Purified Can, Eq, GP, Hu, Ms, Por, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ASPH antibody

  Rabbit Polyclonal Anti-ASPH Antibody  
WB Suggested Anti-ASPH Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: ACHN cell lysate Rabbit IgG Purified Bov, Hu, Ms, Por, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

ASPH antibody

  Rabbit Polyclonal Anti-ASPH  
1Human 721_B; Host: Rabbit; Target Name: ASPH; Sample Tissue: 721_B; Antibody Dilution: 1.0 ug/ml ASPH is supported by BioGPS gene expression data to be expressed in 721_B Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

ASPH antibody

  Rabbit Polyclonal Anti-ASPH  
WB Suggested Anti-ASPH Antibody Titration: 2.5 ug/ml; Positive Control: HepG2 cell lysateThere is BioGPS gene expression data showing that ASPH is expressed in HepG2 Rabbit IgG Purified Can, Eq, Hu, Ms, Por, Rb, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ASPH antibody

  Rabbit Polyclonal Anti-ASPH Antibody  
WB Suggested Anti-ASPH Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Human Stomach Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

ASPH (Center) antibody

ASPH Antibody (Center) (Cat. #AP50276PU-N)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ASPH Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

ASPH - human gene knockout kit via CRISPR

ASPH - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

ASPH - human gene knockout kit via CRISPR

ASPH - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

ASPH - human gene knockout kit via CRISPR

ASPH - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

ASPH - human gene knockout kit via CRISPR

ASPH - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Asph - mouse gene knockout kit via CRISPR

Asph - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Asph - mouse gene knockout kit via CRISPR

Asph - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Asph - mouse gene knockout kit via CRISPR

Asph - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Asph - mouse gene knockout kit via CRISPR

Asph - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ASPH (transcript variant 3)

  Recombinant protein of human aspartate beta-hydroxylase (ASPH), transcript variant 3  
ASPH Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

ASPH (transcript variant 1)

  Recombinant protein of human aspartate beta-hydroxylase (ASPH), transcript variant 1  
ASPH Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

ASPH (transcript variant 2)

  Recombinant protein of human aspartate beta-hydroxylase (ASPH), transcript variant 2  
ASPH Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

ASPH (transcript variant 11)

  Purified recombinant protein of Homo sapiens aspartate beta-hydroxylase (ASPH), transcript variant 11.  
ASPH Human > 80 %
Preparation: or Add: Recombint proteins was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

ASPH (75-270, His-tag)

ASPH Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €1,000.00
  Acris Antibodies GmbH

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