ASPSCR1 antibody

Principal name

ASPSCR1 antibody

Alternative names for ASPSCR1 antibody

ASPL, RCC17, TUG, UBXD9, UBXN9, Tether containing UBX domain for GLUT4, Alveolar soft part sarcoma chromosomal region candidate gene 1 protein, Alveolar soft part sarcoma locus, Renal papillary cell carcinoma protein 17, UBX domain-containing protein 9

SwissProt ID

Q8VBT9 (Mouse), Q9BZE9 (Human)

Gene ID

79058 (ASPSCR1), 68938 (Aspscr1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), EPair for Elisa (EPair), ELISA (detection) (E(detection)), Enzyme Immunoassay (E), Paraffin Sections (P), Immunoprecipitation (IP), Frozen Sections (C)

Background of ASPSCR1 antibody

ASPSCR1 is tethering protein that sequesters GLUT4-containing vesicles in the cytoplasm in the absence of insulin. Modulates the amount of GLUT4 that is available at the cell surface.
Insulin stimulates glucose uptake in fat and muscle by mobilizing the GLUT4 glucose transporter. GLUT4 is sequestered intracellularly in the absence of insulin, and is redistributed to the plasma membrane within minutes of insulin stimulation. Bogan et al. (2003) described a functional screen to identify proteins that modulate GLUT4 distribution, and identified TUG as a putative tether, containing a UBX domain, for GLUT4. They identified the ASPL protein as the probable human homolog of murine TUG. In truncated form, TUG acts in a dominant-negative manner to inhibit insulin-stimulated GLUT4 redistribution in Chinese hamster ovary cells and 3T3-L1 adipocytes. Full-length TUG forms a complex specifically with GLUT4; in 3T3-L1 adipocytes, this complex is present in unstimulated cells and is largely disassembled by insulin. Endogenous TUG is localized with the insulin-mobilizable pool of GLUT4 in unstimulated 3T3-L1 adipocytes, and is not mobilized to the plasma membrane by insulin. Distinct regions of TUG are required to bind GLUT4 and to retain GLUT4 intracellularly in transfected, nonadipose cells. Bogan et al. (2003) concluded that TUG traps endocytosed GLUT4 and tethers it intracellularly, and that insulin mobilizes this pool of retained GLUT4 by releasing this tether. Bogan et al. (2003) found that TUG exists in 2 isoforms, the longer of which contains a sequence at the amino terminus with similarity to ubiquitin that is predicted to be 550 residues long. The probable start codon in the short form is equivalent to methionine-78 of the long form.

General readings

Bogan JS, Hendon N, et al. (2003). Nature. 425(6959):727-33.Argani P, Antonescu CR, et al. (2001). Am J Pathol. 159(1):179-92.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ASPSCR1 antibody

Predicted band size: 70-75 kDa . Positive control: HL60 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lysate: 30 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ASPSCR1 antibody

WB Suggested Anti-ASPSCR1 Antibody; Titration: 1.0 ug/ml; Positive Control: Fetal Lung Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

ASPSCR1 (C-term) antibody

Western blot analysis of ASPSCR1/TUG Antibody (C-term) (Cat.#AP17891PU-N) in Hela cell line lysates (35ug/lane). ASPSCR1 (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

ASPSCR1 (N-term) antibody

ASPSCR1 Antibody (N-term) (Cat. #AP50277PU-N) western blot analysis in A2058 cell line lysates (35µg/lane).This demonstrates the ASPSCR1 antibody detected the ASPSCR1 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

ASPSCR1 antibody

Western Blot analysis of ASPSCR1 expression in transfected 293T cell line (H00079058-T01) by ASPSCR1 MaxPab polyclonal antibody.

Lane 1: ASPSCR1 transfected lysate(60.83 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

ASPSCR1 antibody

ASPSCR1 MaxPab rabbit polyclonal antibody. Western Blot analysis of ASPSCR1 expression in mouse lung. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

ASPSCR1 antibody

Immunoperoxidase of monoclonal antibody to ASPSCR1 on formalin-fixed paraffin-embedded human breast cancer. [antibody concentration 3ug/ml] Mouse IgG1 3D10-1D11 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ASPSCR1 antibody

Western blot analysis using Aspscr1 monoclonal antibody, clone 4A11A6G11 ( Cat # MAB5537 ) against NIH/3T3 cell lysate. Mouse IgG1 4A11A6G11 Ms E, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

ASPSCR1 (Center) antibody

Human Fetal Lung; WB Suggested Anti-ASPSCR1 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Fetal Lung; ASPSCR1 antibody - middle region (ARP61223_P050) in Human Fetal Lung cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

ASPSCR1 antibody

TUG Antibody [NBP1-28709] - Detection of Human ASPL by Western Blot of Immunoprecipitates.  Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells.   Antibodies: Affinity purified rabbit anti-ASPL antibody NBP1-28709 used for IP at 3 mcg/mg lysate.  ASPL was also immunoprecipitated by rabbit anti-ASPL antibody NBP1-28710, which recognizes a downstream epitope.  For blotting immunoprecipitated ASPL, NBP1-28710 was used at 1 mcg/ml, and the ReliaBLOT®  Reagents and Procedures were incorporated.  Detection: Chemiluminescence with an exposure time of 10 seconds. Rabbit IgG Aff - Purified Hu IP
0.1 mg / €440.00
  Novus Biologicals Inc.

ASPSCR1 antibody

TUG Antibody [NBP1-28710] - Detection of Human ASPL by Western Blot of Immunoprecipitates.  Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells.   Antibodies: Affinity purified rabbit anti-ASPL antibody NBP1-28710 used for IP at 3 mcg/mg lysate.  ASPL was also immunoprecipitated by rabbit anti-ASPL antibody NBP1-28709, which recognizes an upstream epitope.  For blotting immunoprecipitated ASPL, NBP1-28710 was used at 1 mcg/ml, and the ReliaBLOT®  Reagents and Procedures were incorporated.  Detection: Chemiluminescence with an exposure time of 10 seconds. Rabbit IgG Aff - Purified Hu IP
0.1 mg / €440.00
  Novus Biologicals Inc.

ASPSCR1 antibody

Western Blot: TUG Antibody (4A11A6G11) [NBP1-47360] - Western blot analysis using anti-Mouse TUG monoclonal antiobdy against NIH/3T3 cell lysate. Mouse IgG1 4A11A6G11 Aff - Purified Ms E, WB
0.1 mg / €380.00
  Novus Biologicals Inc.

ASPSCR1 antibody

Immunohistochemistry-Paraffin: TUG Antibody [NBP1-90079] - Immunohistochemical staining of human ovary shows strong cytoplasmic positivity in follicle cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ASPSCR1

ASPSCR1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

ASPSCR1 (1-553, His-tag)

ASPSCR1 Human Purified > 85 % by SDS - PAGE E. coli
0.1 mg / €730.00
  Acris Antibodies GmbH

ASPSCR1 (1-553, His-tag)

ASPSCR1 Human Purified > 85 % by SDS - PAGE E. coli
20 µg / €300.00
  Acris Antibodies GmbH

ASPSCR1

ASPSCR1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

ASPSCR1

ASPSCR1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Antibody Pairs

Catalog No.    

ASPSCR1 (Human) Matched Antibody Pair

ASPSCR1 (Human) Matched Antibody Pair  
1 set / €710.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

ASPSCR1 293T Cell Transient Overexpression Lysate(Denatured)

ASPSCR1 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

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