ATAD5 antibody

Principal name

ATAD5 antibody

Alternative names for ATAD5 antibody

C17orf41, FRAG1, ATPase family AAA domain-containing protein 5, Chromosome fragility-associated gene 1 protein

SwissProt ID

Q4QY64 (Mouse), Q96QE3 (Human)

Gene ID

79915 (ATAD5), 237877 (Atad5)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Chimp (Chimpanzee)

Available hosts

Rabbit, Goat

Available applications

Western blot / Immunoblot (WB), Immunoprecipitation (IP), PLA/Proximity ligation assay (PLA), Enzyme Immunoassay (E), Paraffin Sections (P)

Background of ATAD5 antibody

The SET domain is a highly conserved, approximately 150-amino acid motif implicated in the modulation of chromatin structure. It was originally identified as part of a larger conserved region present in the Drosophila trithorax protein and was subsequently identified in the Drosophila Su(var)3-9 and 'Enhancer of zeste' proteins, from which the acronym SET is derived. Studies have suggested that the SET domain may be a signature of proteins that modulate transcriptionally active or repressed chromatin states through chromatin remodeling activities. ESET functions as a histone methyltransferase by methylation of Lys-9 of histone H3.

6 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ATAD5 (63-76) antibody

WB using Anti-Elg1 antibody shows detection of a band ~120 kDa corresponding to human Elg1 (arrowhead) in various cell lysates. Lanes contain ~5 ug of HeLa nuclear extract (1), HeLa (2), A431 (3), Jurkat (4) and HEK293 (5) whole cell lysates. Primary antibody was used at 1:500. The expected molecular weight of Elg1 is 120kDa according to Kanellis P et al. 2003. The 50kD bands in Jurkat and 293 cell lysates are probably cross-reaction with other proteins. Rabbit IgG Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ATAD5 antibody

ATAD5 Goat Hu E
0.1 mg / €390.00
  Abnova Taiwan Corp.

ATAD5 antibody

ATAD5 Goat Ms, Rt E
0.1 mg / €390.00
  Abnova Taiwan Corp.

ATAD5 antibody

Western blot using ATAD5 polyclonal antibody ( Cat # PAB10222 ) shows detection of a band ~120 kDa corresponding to human ATAD5 ( arrowhead ) in various cell lysates.
Lanes contain ~ 5 µg of HeLa nuclear extract ( 1 ), HeLa ( 2 ), A-431( 3 ), Jurkat ( 4 ) and HEK293 ( 5 ) whole cell lysates.
After SDS-PAGE, transfer and blocking, the membrane was probed with the primary antibody diluted to 1:500.
The membrane was then washed and reacted with a HRP conjugated Gt-a-Rabbit IgG [H&L] MX followed by ECL detection using a 2m exposure time.
The expected molecular weight of ATAD5 is 120 kDa according to Kanellis P et al. 2003, although the predicted molecular weight is 207 kDa.
The 50 kDa bands in Jurkat and 293 cell lysates are probably cross-reaction with other proteins.
Both the 120 kDa and 50 kDa bands are not observed when antibody is pre-incubated with peptide ( datanot shown ). Chimp, Hu
0.1 mg / €560.00
  Abnova Taiwan Corp.

ATAD5 antibody

Western Blot and Immunoprecipitation: ATAD5/FRAG1 Antibody [NB100-57495] - Detection of Human ATAD5/FRAG1 on HeLa whole cell lysates using NB100-57495. Rabbit Aff - Purified Hu IP, PLA, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

ATAD5 antibody

Western Blot: SETDB1 Antibody [NBP1-77990] detect human ESET present in a 293 whole cell lysate. ~30ug of lysate was loaded per lane for SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a single band of ~170 kDa is detected. Peptide competition (not shown) blocks staining of this band. The blot was incubated with a 1:1000 dilution of the antibody at room temperature for 2 h followed by detection using IRDye800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:5,000 for 45 min. Rabbit Aff - Purified Hu E, P, WB
0.1 mg / €510.00
  Novus Biologicals Inc.

6 Item(s)

per page
  • LinkedIn