ATF1 antibody

Principal name

ATF1 antibody

Alternative names for ATF1 antibody

ATF-1, Activating transcription factor 1, Protein TREB36, Cyclic AMP-dependent transcription factor ATF-1, cAMP-dependent transcription factor ATF-1

SwissProt ID

P18846 (Human), P40353 (Yeast), P81269 (Mouse), Q08DA8 (Bovin)

Gene ID

466 (ATF1), 11908 (Atf1)

Available reactivities

Bov (Bovine), Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), African clawed frog, Ze (Zebrafish), Por (Porcine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Sh (Sheep)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunoprecipitation (IP)

Background of ATF1 antibody

Eukaryotic gene transcription is regulated by sequence-specific transcription factors that bind modular cis acting promoter and enhancer elements. The ATF/ CREB transcription factor family binds the palindromic cAMP response element (CRE) octanucleotide TGACGTCA. The ATF/CREB family includes CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2 and ATF-3. This family of proteins contain highly divergent N-terminal domains, but share a C-terminal leucine zipper for dimerization and DNA binding. ATF-1 is shown to play a key role in the induction of NOX1, this protein binds the cAMP response element (CRE) and mediates PKA-induced stimulation of CRE-reporter genes. ATF-2 forms homodimers and heterodimers with c-Jun to initiate CREdependent transcription. Phosphorylation of ATF-2 at Thr 69 and Thr 71 by stress-activated kinases is necessary for transcriptional activation. Myc also induces phosphorylation of ATF-2 at Thr 69 and Thr 71 to prolong the half-life of ATF-2. ATF-2 functions as a histone acetyltransferase (HAT) and acetylates Histones H2B and H4 specifically in vitro.

General readings

Lin Y S., et al. (1988) Proc Natl Acad Sci USA. 85:3396-3400.Gupta P., et al. (2002) J Biol Chem. 277:50550-50556.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ATF1 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ATF1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA807630)(1:150) Mouse IgG2b OTI2B4 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ATF1 antibody

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-ATF1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807697)(1:150) Mouse IgG2a OTI7H3 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ATF1 antibody

Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 3E7 Purified Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

ATF1 antibody

Western blot analysis of extracts from COLO205 cells, HeLa cells and HepG2 cells, using ATF1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ATF1 antibody

Western blot analysis of ATF1 expression in COLO205 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ATF1 antibody

Western blot analysis of ATF1 phosphorylation expression in Insulin treated HT29 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ATF1 antibody

Host: Rabbit; Target Name: ATF1; Sample Tissue: Fetal Brain lysates; Antibody Dilution: 1.0ug/ml Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

ATF1 antibody

WB Suggested Anti-ATF1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: 721_B cell lysateATF1 is supported by BioGPS gene expression data to be expressed in 721_B Rabbit IgG Purified Hu P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

ATF1 antibody

WB Suggested Anti-ATF1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Thymus Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Sh WB
50 µg / €325.00
  OriGene Technologies, Inc.

ATF1 antibody

WB Suggested Anti-ATF1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 12500; Positive Control: Human Placenta Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Sh, Ze P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

ATF1 antibody

ATF1 Mouse IgG2a ATF1 2A9/8 Purified Hu IP, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ATF1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATF1 (RC205923, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATF1.(1:2000) Mouse IgG1 OTI1D4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ATF1 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ATF1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA807623)(1:150) Mouse IgG1 OTI2C2 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ATF1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATF1 (RC205923, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATF1.(1:2000) Mouse IgG2b OTI6B7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ATF1 antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ATF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807699) Mouse IgG1 OTI5D10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ATF1 antibody

Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-ATF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807704) Mouse IgG1 OTI8G3 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ATF1 antibody

Western blot (WB) analysis of ATF1 antibody (Cat.-No.: AP06680PU-N) at 1/500 dilution Lane1:Hela whole cell lysate Lane2:sp2/0 whole cell lysate Lane3:H9C2 whole cell lysate Lane4:Mouse brain tissue lysate Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

ATF1 antibody

Immunohistochemistry (IHC) analyzes of ATF-1 antibody (Cat.-No.: AP21087PU-N) in paraffin-embedded human lung carcinoma tissue. Rabbit Aff - Purified Hu, Ms P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

ATF1 pSer63 antibody

Western blot analysis of extracts from HT29 cells treated with Insulin using Phospho-ATF1 (Ser63) antibody AP55869PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms WB
0.1 ml / €340.00
  Acris Antibodies GmbH

ATF1 pSer63 antibody

Western blot analysis of extracts from HT29 cells treated with Insulin using Phospho-ATF1 (Ser63) antibody AP55869PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms WB
50 µl / €270.00
  Acris Antibodies GmbH

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