ATF1 antibody

Principal name

ATF1 antibody

Alternative names for ATF1 antibody

ATF-1, Activating transcription factor 1, Protein TREB36, Cyclic AMP-dependent transcription factor ATF-1, cAMP-dependent transcription factor ATF-1

SwissProt ID

P18846 (Human), P40353 (Yeast), P81269 (Mouse), Q08DA8 (Bovin)

Gene ID

466 (ATF1)

Available reactivities

Bov (Bovine), Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), African clawed frog, Ye (Yeast), Ze (Zebrafish), Por (Porcine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Sh (Sheep)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), EPair for Elisa (EPair)

Background of ATF1 antibody

ATF1 (Activating Transcription Factor 1, TREB-36) is a member of the ATF/CREB family of basic region leucine-zipper (bZip) DNA-binding proteins that regulates transcription by binding to a consensus cAMP response element (CRE) in the promoter of various viral and cellular genes. Many of these genes are important in cell growth and differentiation, and in stress and immune responses. The activation function of CRE-binding proteins may be modulated by phosphorylation of several kinases and is mediated by coactivators such as CREB-binding protein (CBP) and p300. ATF1 is a nuclear protein that binds DNA as a homodimer or as heterodimers with the inducible transcription factors CREB1 or CREM. Heterodimers appear to be stronger transcriptional activators than the homodimers. Tissue expression of ATF1 mRNA is widespread. Several isoforms of ATF1 arise by differential splicing. ATF1 mediates both Ca2+ and cAMP responses at several levels. It binds to the Tax-responsive element (TRE1) of the human T-cell lymphotropic virus type-I (HTLV1). ATF1 is detectable in metastatic melanoma cells and seems to contribute to their survival. A chimeric protein composed of the N-terminal domain of EWS (Ewing sarcoma oncogene) linked to the bZip domain of ATF1 is implicated in the rare malignant clear cell sarcoma of tendon sheath and aponeuroses (malignant melanoma of soft parts).

General readings

Lin Y S., et al. (1988) Proc Natl Acad Sci USA. 85:3396-3400.Gupta P., et al. (2002) J Biol Chem. 277:50550-50556.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ATF1 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ATF1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA807630)(1:150) Mouse IgG2b OTI2B4 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ATF1 antibody

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-ATF1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807697)(1:150) Mouse IgG2a OTI7H3 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ATF1 antibody

Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 3E7 Purified Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

ATF1 antibody

Western blot analysis of extracts from COLO cells, using ATF1 (Ab-63) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ATF1 antibody

Western blot analysis of extracts from COLO205 cells, HeLa cells and HepG2 cells, using ATF1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ATF1 antibody

Western blot analysis of ATF1 expression in COLO205 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ATF1 antibody

WB Suggested Anti-ATF1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Thymus Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Sh WB
50 µg / €325.00
  OriGene Technologies, Inc.

ATF1 antibody

WB Suggested Anti-ATF1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 12500; Positive Control: Human Placenta Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Sh, Ze P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

ATF1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATF1 (RC205923, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATF1.(1:2000) Mouse IgG1 OTI1D4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ATF1 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ATF1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA807623)(1:150) Mouse IgG1 OTI2C2 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ATF1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATF1 (RC205923, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATF1.(1:2000) Mouse IgG2b OTI6B7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ATF1 antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ATF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807699) Mouse IgG1 OTI5D10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ATF1 antibody

Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-ATF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807704) Mouse IgG1 OTI8G3 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ATF1 antibody

Western blot (WB) analysis of ATF1 antibody (Cat.-No.: AP06680PU-N) at 1/500 dilution Lane1:Hela whole cell lysate Lane2:sp2/0 whole cell lysate Lane3:H9C2 whole cell lysate Lane4:Mouse brain tissue lysate Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

ATF1 antibody

Immunohistochemistry (IHC) analyzes of ATF-1 antibody (Cat.-No.: AP21087PU-N) in paraffin-embedded human lung carcinoma tissue. Rabbit Aff - Purified Hu, Ms P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

ATF1 pSer63 antibody

Western blot analysis of extracts from HT29 cells treated with Insulin using Phospho-ATF1 (Ser63) antibody AP55869PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms WB
0.1 ml / €340.00
  Acris Antibodies GmbH

ATF1 pSer63 antibody

Western blot analysis of extracts from HT29 cells treated with Insulin using Phospho-ATF1 (Ser63) antibody AP55869PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms WB
50 µl / €270.00
  Acris Antibodies GmbH

ATF1 antibody

Western Blot analysis of ATF1 expression in transfected 293T cell line (H00000466-T02) by ATF1 MaxPab polyclonal antibody.

Lane 1: ATF1 transfected lysate(29.20 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

ATF1 antibody

ATF1 Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

ATF1 antibody

ATF1 MaxPab rabbit polyclonal antibody. Western Blot analysis of ATF1 expression in mouse brain. Rabbit Purified Hu, Ms, Rt E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

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