ATF2 antibody

Principal name

ATF2 antibody

Alternative names for ATF2 antibody

ATF-2, CREB2, CREBP1, Activating transcription factor 2, CRE-BP1, HB16, CREB-2, Cyclic AMP-dependent transcription factor ATF-2, cAMP-dependent transcription factor ATF-2, cAMP response element-binding protein CRE-BP1, Cyclic AMP-responsive element-binding protein 2

SwissProt ID

O93602 (Chick), P15336 (Human), P16951 (Mouse), Q00969 (Rat)

Gene ID

1386 (ATF2), 81647 (Atf2), 11909

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), Chk (Chicken), African clawed frog, Mky (Monkey), Ze (Zebrafish)

Available hosts

Rabbit, Sheep, Mouse, Goat

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Dot blot (Dot), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Electrophoretic Mobility Shift Assay (EMSA)

Background of ATF2 antibody

ATF2 (Activating Transcription Factor 2, CREBP, HB16, CREB2, TREB7) is a member of the ATF/CREB family of basic region leucine zipper DNA binding proteins that regulates transcription by binding to a consensus cAMP response element (CRE) in the promoter of various viral and cellular genes. Many of these genes are important in cell growth and differentiation, and in stress and immune responses. ATF2 is a nuclear protein that binds DNA as a dimer and can form dimers with members of the ATF/CREB and Jun/Fos families. It is a stronger activator as a heterodimer with cJun than as a homodimer. Several isoforms of ATF2 arise by differential splicing. The stable native full length ATF2 is transcriptionally inactive as a result of an inhibitory direct intramolecular interaction of its carboxy terminal DNA binding domain with the amino terminal transactivation domain. Following dimerization ATF2 becomes a short lived protein that undergoes ubiquitination and proteolysis, seemingly in a protein phosphatase-dependent mechanism. Stimulation of the transcriptional activity of ATF2 occurs following cellular stress induced by several genotoxic agents, inflammatory cytokines, and UV irradiation. This activation requires phosphorylation of two threonine residues in ATF2 by both JNK/SAP kinase and p38 MAP kinase. ATF2 is abundantly expressed in brain.

General readings

Bhoumik, A., Jones, N. and Ronai, Z. "Transcriptional switch by activating transcription factor 2-derived peptide sensitizes melanoma cells to apoptosis and inhibits their tumorigenicity,"
Proc. Natl. Acad. Sci. U.S.A. 101 (2004) 4222 - 4227.
Gutpa, S., Campbell, D., Derijard, B. and Davis, R.
"Transcription factor ATF2 regulation by the JNK signal transduction pathway," Science 267 (1995) 389 - 393.
Ivanov, V.N., Bhoumik, A. and Ronai, Z. "Death receptors and melanoma resistance to apoptosis," Oncogene 22 (2003) 3152 - 3161.
Karin, M. and Hunter, T. "Transcriptional control by protein phosphorylation: signal transmission from the cell surface to the nucleus," Curr. Biol. 5 (1995) 747 - 757.
Laifenfeld, D., Karry, R., Grauer, E., Klein, E. and Ben-Shachar, D. "ATF2, a member of the CREB/ATF family of transcription factors, in chronicstress and consequent to
antidepressant treatment: animal models and humanpost-mortem brains," Neuropsychopharmacology 29 (2004) 589 - 97.
Maekawa, T., Sakura, H., Kanei-Ishii, C., Sudo, T., Yoshimura, T. and Fujisawa, J. "Leucine zipper structure of the protein CRE-BP1 binding to the cyclic AMP response element in brain," EMBO J. 8 (1989) 2023 - 2028.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ATF2 pSer490/498 antibody

Figure 1. Western blot of Human melanoma cells incubated with varying doses of the radiomimetic drug NCS showing specific immuno-labeling of the ~74k ATF2 protein phosphorylated at Ser490 and Ser498. Rabbit IgG Aff - Purified Hu, Rt C, WB
0.1 ml / €380.00
  Acris Antibodies GmbH

ATF2 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATF-2 antibody. Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €270.00
  Acris Antibodies GmbH

ATF2 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATF-2 antibody. Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
50 µl / €230.00
  Acris Antibodies GmbH

ATF2 antibody

Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using ATF-2 antibody. Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €270.00
  Acris Antibodies GmbH

ATF2 antibody

Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using ATF-2 antibody. Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
50 µl / €230.00
  Acris Antibodies GmbH

ATF2 (C-term) antibody

TA302732 staining (0.5µg/ml) of Human Placenta lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 12 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ATF2 antibody

Western blot analysis of extracts from LOVO cells, using ATF2 Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ATF2 antibody

Western blot analysis of extracts from HeLa cells, using ATF2 (Ab-71 or 53) Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ATF2 antibody

Predicted cell location: Nucleus. Positive control: Human lymphoma and lung cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human lymphoma cancer tissue using ATF2 antibody at dilution 1/15, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

ATF2 antibody

Predicted cell location: Nucleus. Positive control: Human lung cancer tissue. Recommended dilution: 1/15-50  The image on the left is immunohistochemistry of paraffin-embedded human lung cancer tissue using ATF2 antibody at dilution 1/15, on the right is treated with the fusion protein. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

ATF2 antibody

ATF2 Antibody (pT339) (Cat. #TA324608) western blot analysis in Hela cell line and mouse brain tissue lysates (35ug/lane).This demonstrates the ATF2 antibody detected the ATF2 protein (arrow). Rabbit IgG Purified Hu, Ms WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

ATF2 antibody

Western blot analysis of ATF2 expression in HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ATF2 antibody

Western blot analysis of ATF2 expression in MDA-MB-435 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ATF2 antibody

Western blot analysis of extracts from HeLa cells and 293 cells, using ATF-2 (Ab-472) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ATF2 antibody

Western blot analysis of extracts of HeLa cell lines, using ATF2 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ATF2 antibody

Flow Cytometry: ATF2 [p Thr71] Antibody (103C411.2) [TA336422] - Detection of ATF2 phosphorylation in HeLa cell lysate with phospho ATF2 antibody. Lane A. Untreated cell lysate; Lane B. HeLa cells treated with TNF for 1 hr. Mouse IgG1 103C411.2 Purified Hu F, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ATF2 antibody

Immunohistochemical analysis of paraffin-embedded lung cancer (left) and brain tissues (right) using ATF2 antibody Cat.-No AM06576SU-N with DAB staining. Mouse IgG1 4F12 Ascites Hu, Ms E, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

ATF2 (241-340) antibody

Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 4A3 Purified Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

ATF2 pThr69/51 + pThr71/53 antibody

ATF2 Rabbit Purified Hu, Ms, Rt IP, P, WB
0.1 mg / €460.00
  Acris Antibodies GmbH

ATF2 pSer112/94 antibody

ATF2 Rabbit Aff - Purified Hu, Ms, Rt E, IP, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

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