ATF3 antibody

Principal name

ATF3 antibody

Alternative names for ATF3 antibody

Activating transcription factor 3, ATF-3, Cyclic AMP-dependent transcription factor ATF-3, cAMP-dependent transcription factor ATF-3

SwissProt ID

P18847 (Human), P29596 (Rat), Q2KII1 (Bovin), Q60765 (Mouse)

Gene ID

467 (ATF3)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mam (Mammalia), Bov (Bovine), Can (Canine), Chk (Chicken), African clawed frog, Por (Porcine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Ye (Yeast), Ze (Zebrafish)

Available hosts

Sheep, Mouse, Rabbit

Available applications

Immunoprecipitation (IP), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of ATF3 antibody

This protein binds the cAMP response element (CRE) (consensus: 5\'-GTGACGT[AC][AG]-3\'), a sequence present in many viral and cellular promoters. ATF3 represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters.Activating transcription factor 3 is a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. Multiple transcript variants encoding two different isoforms have been found for this gene. The longer isoform represses rather than activates transcription from promoters with ATF binding elements. The shorter isoform (deltaZip2) lacks the leucine zipper protein-dimerization motif and does not bind to DNA, and it stimulates transcription presumably by sequestering inhibitory co-factors away from the promoter. It is possible that alternative splicing of the ATF3 gene may be physiologically important in the regulation of target genes.

General readings

Sampson,M.J., (2008) Eur. J. Endocrinol. 158 (5), 729-739

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ATF3 antibody

Western blot analysis of ATF3 over-expressed 293 cell line, cotransfected with ATF3 Validated Chimera RNAi ( Cat # H00000467-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ATF3 monoclonal antibody (M01), clone 6B8 (Cat # H00000467-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. Mouse IgG3 6B8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ATF3 antibody

Western blot analysis of extracts from RAW264.7 cells, using ATF3 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ATF3 (113-130) antibody

WB of mammalian whole cell extract transfected with HA epitope tagged human ATF3. Anti-ATF3 detects a band ~31 kDa corresponding to recombinant human ATF3. The membrane was probed with the primary antibody diluted 1:200 in 0.2X blocking buffer in PBS overnight at 4°C. Reaction was followed by washes and reaction with a 1:5000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L]  for 30 min at room temperature. Rabbit IgG Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ATF3 antibody

Western blot analysis of extracts of various cell lines, using ATF3 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ATF3 antibody

Western Blot: ATF3 Antibody [TA336705] - Western blot analysis of ATF3 on UV treated NIH/3T3 cell extracts using TA336705. Rabbit Purified Hu, Mam, Ms, Rt C, E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ATF3 antibody

WB Suggested Anti-ATF3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: OVCAR-3 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ye, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

ATF3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATF3 (RC202897, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATF3.(1:2000) Mouse IgG2b OTI5G9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ATF3 antibody

AP07545PU-N ATF3 antibody staining of Formalin-Fixed Paraffin-Embedded Human Prostate at 5 µg/ml followed by biotinylated Goat anti-Rabbit IgG secondary antibody, Alkaline Phosphatase-Streptavidin and Chromogen. Rabbit Aff - Purified Hu, Mam E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

ATF3 (C-term) antibody

ATF3 Antibody (C-term) (Cat. #AP50284PU-N) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the ATF3 antibody detected the ATF3 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

ATF3 antibody

ATF3 Sheep Purified Hu, Ms, Rt IP
0.2 mg / €360.00
  Acris Antibodies GmbH

ATF3 antibody

ATF3 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

ATF3 antibody

Western Blot analysis of ATF3 expression in transfected 293T cell line (H00000467-T01) by ATF3 MaxPab polyclonal antibody.

Lane 1: ATF3 transfected lysate(19.91 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

ATF3 antibody

Western Blot analysis of ATF3 expression in transfected 293T cell line (H00000467-T01) by ATF3 MaxPab polyclonal antibody.

Lane 1: ATF3 transfected lysate(20.60 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

ATF3 antibody

ATF3 monoclonal antibody (M02), clone 8D8 Western Blot analysis of ATF3 expression in Hela NE ( Cat # L013V3 ). Mouse IgG1 8D8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ATF3 antibody

ATF3 monoclonal antibody (M04), clone 8G5 Western Blot analysis of ATF3 expression in Hela NE ( Cat # L013V3 ). Mouse IgG3 8G5 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ATF3 antibody

ATF3 monoclonal antibody (M06), clone 7G10 Western Blot analysis of ATF3 expression in Hela NE ( Cat # L013V3 ). Mouse IgG1 7G10 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ATF3 antibody

ATF3 monoclonal antibody (M07), clone 6G11 Western Blot analysis of ATF3 expression in Hela NE ( Cat # L013V3 ). Mouse IgG3 6G11 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ATF3 antibody

ATF3 Hu
0.1 mg / €560.00
  Abnova Taiwan Corp.

ATF3 antibody

Western blot analysis of extracts from RAW264.7 cells, using ATF3 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

ATF3 antibody

Western blot analysis of extracts from RAW264.7 cells, using ATF3 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

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