ATF4 antibody

Principal name

ATF4 antibody

Alternative names for ATF4 antibody

CREB2, CREB-2, TXREB, Activating transcription factor 4, ATF-4, Cyclic AMP-dependent transcription factor ATF-4, DNA-binding protein TAXREB67, Cyclic AMP-responsive element-binding protein 2, cAMP-responsive element-binding protein 2

SwissProt ID

P18848 (Human), Q06507 (Mouse), Q3ZCH6 (Bovin), Q9ES19 (Rat)

Gene ID

468 (ATF4), 643159 (ATF4P3), 11911

Available reactivities

Hu (Human), Ms (Mouse), Por (Porcine), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), Sh (Sheep), GP (Guinea Pig)

Available hosts

Rabbit, Goat, Mouse

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Flow Cytometry (F), Immunoprecipitation (IP), ChIP assay (ChIP), EPair for Elisa (EPair), ELISA (detection) (E(detection))

Background of ATF4 antibody

ATF4s a transcription factor that was origilly identified as a widely expressed mammalian D binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of D-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-termil to a stretch of basic amino acids that functions as a D binding domain. Two altertive transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromsome at q28 in a region containing a large inverted duplication.This gene encodes a transcription factor that was origilly identified as a widely expressed mammalian D binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of D-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-termil to a stretch of basic amino acids that functions as a D binding domain. Two altertive transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromsome at q28 in a region containing a large inverted duplication.

General readings

1. Zhu H  et al. Activating transcription factor 4 promotes esophageal squamous cell carcinoma invasion and metastasis in mice and is associated with poor prognosis in human patients. PLoS One 9:e103882 (2014). IHC-P ; Human .
2. Lenna S  et al. HLA-B35 and dsRNA induce endothelin-1 via activation of ATF4 in human microvascular endothelial cells. PLoS One 8:e56123 (2013). IHC-P ; Human .
3. Lenna S  et al. HLA-B35 and dsRNA induce endothelin-1 via activation of ATF4 in human microvascular endothelial cells. PLoS One 8:e56123 (2013). IHC-P ; Human .
4. Ohri SS  et al. Deletion of the pro-apoptotic endoplasmic reticulum stress response effector CHOP does not result in improved locomotor function after severe contusive spinal cord injury. J Neurotrauma : (2011). ICC/IF ; Mouse .
5. Ameri K  et al. Circulating tumour cells demonstrate an altered response to hypoxia and an aggressive phenotype. Br J Cancer 102:561-9 (2010). IHC-P ; Human .

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ATF4 antibody

Western blot analysis of extracts of various cell lines, using ATF4 antibody. Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ATF4 antibody

Western blot analysis of ATF4 (arrow) using rabbit anti-ATF4 Antibody (S245) (Cat. #TA302232). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ATF4 gene (Lane 2) (Origene Technologies). Rabbit Ig Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ATF4 (C-term) antibody

TA302638 staining (1µg/ml) of Raji lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Hu, Ms, Por, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ATF4 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ATF4 (Ab-245) Antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

ATF4 (Center) antibody

ATF4 Antibody (Center) (Cat. #TA324485) western blot analysis in Jurkat,MCF-7 cell line lysates (35ug/lane).This demonstrates the ATF4 antibody detected the ATF4 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

ATF4 antibody

Western blot analysis of extracts from A549 cells and Jurkat cells, using ATF-4 (Ab-219) Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ATF4 antibody

WB Suggested Anti-ATF4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Human Muscle Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

ATF4 antibody

WB Suggested Anti-ATF4 Antibody Titration: 5.0 ug/ml; ELISA Titer: 1: 62500; Positive Control: Jurkat cell lysateATF4 is supported by BioGPS gene expression data to be expressed in Jurkat Rabbit IgG Purified Bov, Can, Eq, Hu, Por, Rt, Sh WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ATF4 antibody

WB Suggested Anti-ATF4 Antibody Titration: 0.125 ug/ml; ELISA Titer: 1:62500; Positive Control: NIH/3T3 cell lysate Rabbit IgG Purified Ms P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

ATF4 antibody

WB Suggested Anti-Atf4 Antibody Titration: 0.2-1 ug/ml; Positive Control: Mouse Brain Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rt, Sh ICC/IF, WB
50 µg / €325.00
  OriGene Technologies, Inc.

ATF4 antibody

ATF4 Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

ATF4 antibody

Transfected cell immunoblot: extracts of COS cells transiently transfected with HA-tagged ATF4 or untaggedATF5 or Kv2.1 plasmid and probed with N360A/24 TC supe (left), Rabbit anti-ATF5 (middle) or K89/34 TC supe (right). Mouse IgG2a N360A/24 Supernatant Hu, Rt C, ICC/IF, WB
5 ml / €320.00
  Antibodies Incorporated

NeuroMab

ATF4 antibody

Transfected cell immunoblot: extracts of COS cells transiently transfected with HA-tagged ATF4 or untaggedATF5 or Kv2.1 plasmid and probed with N360A/24 TC supe (left), Rabbit anti-ATF5 (middle) or K89/34 TC supe (right). Mouse IgG2a N360A/24 Purified Hu, Rt C, ICC/IF, WB
0.1 ml / €380.00
  Antibodies Incorporated

NeuroMab

ATF4 (25-327) antibody

ATF4 Mouse IgG2a S360-A-24 Purified Hu, Rt ICC/IF, P, WB
0.1 mg / €380.00
  Acris Antibodies GmbH

ATF4 pSer245 antibody

Immunohistochemistry (IHC) analysis of p-ATF4 (pSer245) antibody Cat.-No.: AP01533PU-N in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu IP, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

ATF4 pSer245 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using ATF4 (phospho-Ser245) antibody AP02352PU. Rabbit Aff - Purified Hu P
0.1 ml / €320.00
  Acris Antibodies GmbH

ATF4 pSer245 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using ATF4 (phospho-Ser245) antibody AP02352PU. Rabbit Aff - Purified Hu P
50 µl / €240.00
  Acris Antibodies GmbH

ATF4 antibody

Immunohistochemistry (IHC) analysis of ATF4 antibody (Cat.-No.: AP06024PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Rt E, P
0.1 mg / €350.00
  Acris Antibodies GmbH

ATF4 antibody

Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE). Rabbit IgG Purified Hu, Ms, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

ATF4 antibody

ATF4 Rabbit Serum Ms C, E, WB
0.2 ml / €400.00
  Acris Antibodies GmbH

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