ATF6 alpha antibody

Principal name

ATF6 alpha antibody

Alternative names for ATF6 alpha antibody

ATF-6, Activating transcription factor 6 alpha, Cyclic AMP-dependent transcription factor ATF-6 alpha, cAMP-dependent transcription factor ATF-6 alpha

SwissProt ID

B2RU98 (Mouse), F6VAN0, P18850 (Human)

Gene ID

22926 (ATF6), 226641 (Atf6)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Eq (Equine), Hst (Hamster), Mky (Monkey), Por (Porcine), Prim (Primate), Rb (Rabbit), Bear, Can (Canine), Rt (Rat), Fi (Fish)

Available hosts

Mouse, Rabbit, Goat

Available applications

ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunoprecipitation (IP), Frozen Sections (C), ChIP assay (ChIP), Flow Cytometry (F)

Background of ATF6 alpha antibody

ATF6 is a constitutively expressed, endoplasmic reticulum (ER) membrane-anchored transcription factor. ATF6 is a key transcriptiol activator of the unfolded protein response (UPR), which allows mammalian cells to maintain cellular homeostasis when they are subjected to environmental and physiological stresses that target the ER (reviewed in Shen, 2005 & Prywes, 2005). The C-terminus of ATF6 is located in the ER lumen and its N-termil D binding domain faces the cytosol. AFT6 plays a key role in the ER stress response by transmitting the ER stress sigl across the ER membrane into the nucleus. The induction of new gene expression by ATF6 is an important aspect of the ER stress response. In response to certain stress conditions, ATF6 translocates from the ER to the Golgi. The 90 kDa full-length ATF6 is processed within the Golgi to its active 50 kDa form through sequential cleavage by site-1 and site-2 proteases (S1P and S2P). Proteolytic activation of ATF6 in the ER stress response is a mechanism to regulate membrane-bound factors, and is referred to as regulated intramembrane proteolysis. The N-termil active ATF6 translocates to the nucleus where it binds to ER stress-response elements in ER stress-response genes (ERSRGs). ATF6 is a potent transcriptiol activator of ERSRGs. The fully glycosylated form of ATF6, a 670 amino acid protein, exhibits an electrophoretic mobility of ~90 kDa in deturing SDS-gels, in part because of the glycosylated modifications. ATF6 has 3 consensus sites for N-linked glycosylation and exists constitutively as a glycosylated protein. Differentially glycosylated ATF6 forms may result from mutations or experimental treatment.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ATF6 alpha antibody

  ATF6 Rabbit Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using ATF6 antibody. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ATF6 alpha antibody

  Mouse Monoclonal ATF6 Antibody (70B1413.1)  
Western Blot: ATF6 Antibody (70B1413.1) [TA336753] - Analysis of ATF6 in NIH3T3 cell lysate using TA336753 at 3 ug/ml. A band corresponding to full-length ATF6 was detected. We have not characterized the ~36 kDa observed band; it may be an ATF6 breakdown/ Mouse IgG1 70B1413.1 Purified Fi, Hst, Hu, Ms, Rb, Rt C, ChIP, F, ICC/IF, IP, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ATF6 alpha (437-449) antibody

Staining of HeLa lysate using ATF6 Antibody Cat.-No AP32822PU-N at 2 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Por, Rt E, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

ATF6 alpha (C-term) antibody

  Rabbit Polyclonal ATF6 Antibody  
Western blot analysis of ATF6 in MCF7 cell lysate with ATF6 antibody at (A) 0.5 and (B) 1 ug/mL. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ATF6 alpha (N-term) antibody

  Rabbit Polyclonal ATF6 Antibody  
Western blot analysis of ATF6 in MDA-MB-361 cell lysate with ATF6 antibody at (A) 0.5 and (B) 1 ug/mL. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ATF6 alpha antibody

  Rabbit Polyclonal Anti-Atf6 Antibody  
WB Suggested Anti-Atf6 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Mouse Heart Rabbit IgG Purified Ms P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

ATF6 alpha antibody

  Rabbit Polyclonal ATF6 Antibody  
Simple Western: ATF6 Antibody [TA336820] - Simple Western lane view shows a specific band detected for ATF 6 in HeLa lysate.  This experiment was performed under reducing conditions using the Wes or Sally Sue separation system 12-230kDa (or 66-440kDa). Rabbit Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ATF6 alpha antibody

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 3D5 Purified Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

ATF6 alpha antibody

AP08807PU-N staining of ATF6 in Breast tissue by Immunohistochemistry (Formalin-Fixed Paraffin-Embedded). Rabbit IgG Purified Hu, Ms E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

ATF6 alpha antibody

Testis: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Purified Hu, Ms ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

ATF6 alpha (Center) antibody

AP17892PU-N ATF6 Antibody (Center) staining of Formalin-Fixed and Paraffin-Embedded Human brain tissue. This antibody was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

ATF6 alpha (C-term) antibody

Human Uterus (formalin-fixed, paraffin-embedded) stained with ATF6 antibody AP23812PU-N at 2.5 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Goat Aff - Purified Bear, Bov, Elephant, Eq, Hst, Hu, Mky, Ms, Por, Prim, Rb E, P
50 µg / €470.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

ATF6 - human gene knockout kit via CRISPR

ATF6 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

ATF6 - human gene knockout kit via CRISPR

ATF6 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

ATF6 - human gene knockout kit via CRISPR

ATF6 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

ATF6 - human gene knockout kit via CRISPR

ATF6 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, ATF6 (Myc-DDK-tagged)-Human activating transcription factor 6 (ATF6), 200 uL, >10^7 TU/mL

Lenti ORF particles, ATF6 (Myc-DDK-tagged)-Human activating transcription factor 6 (ATF6), 200 uL, >10^7 TU/mL  
200 µl / €1,370.00
  OriGene Technologies, Inc.

17 Item(s)

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