ATF7 antibody

Principal name

ATF7 antibody

Alternative names for ATF7 antibody

ATF-7, ATFA, Activating transcription factor 7, ATF-A, Cyclic AMP-dependent transcription factor ATF-7, cAMP-dependent transcription factor ATF-7

SwissProt ID

P17544 (Human), Q8R0S1 (Mouse)

Gene ID

11016 (ATF7)

Available reactivities

Hu (Human), Can (Canine), Ms (Mouse), Rt (Rat), Bov (Bovine), Por (Porcine), Rb (Rabbit), Eq (Equine), GP (Guinea Pig)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Dot blot (Dot), Frozen Sections (C)

Background of ATF7 antibody

Eukaryotic gene transcription is regulated by sequence-specific transcription factors, which bind modular cis acting promoter and enhancer elements. The cAMP response element (CRE) consists of the palindromic octanucleotide TGACGTCA. There are several CRE binding proteins within the ATF/CREB family, including CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2 and ATF-3. A novel basic leucine zipper (bZIP) protein, designated ATF-7, is closely related to members of the ATF/CREB family of bZIP proteins, with highest homology to ATF-4. ATF-7 physically interacts with the PRL-1 protein-tyrosine phosphatase (PTPase), which is a predominately nuclear, farnesylated PTPase. ATF-7 homodimers bind specifically to CRE elements. ATF-7 is expressed in a number of different tissues and is expressed in association with differentiation. ATF-7 and PRL-1 interact with each other through the bZIP region of ATF-7 and the phosphatase domain of PRL-1. In addition, PRL-1 is able to dephosphorylate ATF-7 in vitro.

General readings

Peters CS, Liang X, Li S, Kannan S, Peng Y, Taub R, Diamond RH.
ATF-7, a novel bZIP protein, interacts with the PRL-1 protein-tyrosine phosphatase.
J Biol Chem 2001 Apr 27;276(17):13718-26
PMID: 11278933

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ATF7 (C-term) antibody

TA302744 staining (0.5µg/ml) of HeLa lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ATF7 antibody

Western blot analysis of extracts from HeLa cells and HuvEc cells, using ATF7 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ATF7 antibody

WB Suggested Anti-ATF7 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Human heart Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

ATF7 antibody

WB Suggested Anti-ATF7 Antibody Titration: 2.5 ug/ml; ELISA Titer: 1: 62500; Positive Control: Jurkat cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ATF7 antibody

Immunohistochemistry (IHC) analyzes of ATF-7 antibody (Cat.-No.: AP06675PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

ATF7 (482-494) antibody

Testis: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat IgG Aff - Purified Bear, Bov, Bt, Can, Elephant, Eq, Hst, Hu, L, Ms, Por, Prim, Rb, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

ATF7 antibody

ATF7 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

ATF7 antibody

Western Blot analysis of ATF7 expression in transfected 293T cell line (H00011016-T01) by ATF7 MaxPab polyclonal antibody.

Lane 1: ATF7 transfected lysate(53.13 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

ATF7 antibody

Western Blot analysis of ATF7 expression in transfected 293T cell line (H00011016-T01) by ATF7 MaxPab polyclonal antibody.

Lane 1: ATF7 transfected lysate(53.13 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

ATF7 antibody

ATF7 polyclonal antibody ( Cat # PAB6078 ) staining ( 0.5 µg/ml ) of HeLa lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

ATF7 antibody

Western blot analysis of extracts from HeLa/HuvEc cells, using ATF7 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
50 µg / €205.00
  Assay Biotech

ATF7 antibody

Western blot analysis of extracts from HeLa/HuvEc cells, using ATF7 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
0.1 mg / €310.00
  Assay Biotech

ATF7 antibody

Human Jurkat; WB Suggested Anti-ATF7 Antibody Titration: 2.5ug/ml. ELISA Titer: 1:62500. Positive Control: Jurkat cell lysate; ATF7 antibody - C-terminal region (ARP38869_T100) in Human Jurkat cells using Western Blot Rabbit Aff - Purified Can, Hu, Ms, Rt WB
0.1 mg / €310.00
  AVIVA Systems Biology

ATF7 antibody

Human Heart; WB Suggested Anti-ATF7 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human heart; ATF7 antibody - N-terminal region (P100817_P050) in Human Heart cells using Western Blot Rabbit Aff - Purified Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

ATF7 antibody

Immunofluorescent analysis of ATF7 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Bov, Hu, Ms, Por, Rb, Rt ICC/IF, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

ATF7 antibody

Immunofluorescent analysis of ATF7 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Bov, Hu, Ms, Por, Rb, Rt ICC/IF, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

ATF7 antibody

Immunofluorescent analysis of ATF7 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Bov, Hu, Ms, Por, Rb, Rt ICC/IF, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

ATF7 antibody

Immunohistochemistry: ATF7 Antibody[NB300-899]- (3.8ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat IgG Aff - Purified Hu E, P, WB

Regular Price: 0.1 mg / €400.00

Special Price: 0.1 mg / €238.00

  Novus Biologicals Inc.

ATF7 antibody

Western Blot: ATF7 Antibody [NBP1-30899] - Whole cell lysate treated with ATF7 Antibody, diluted at 1:1500 with 12% SDS PAGE gel. Rabbit IgG Hu Dot, ICC/IF, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

ATF7 antibody

Immunohistochemistry-Paraffin: ATF7 Antibody [NBP1-88876] - Immunohistochemical staining of human cervix, uterine shows moderate nuclear positivity in glandular cells. Rabbit IgG Aff - Purified Hu ICC/IF, P
0.1 ml / €500.00
  Novus Biologicals Inc.

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