ATG12 / APG12 antibody

Principal name

ATG12 / APG12 antibody

Alternative names for ATG12 / APG12 antibody

APG12L, Autophagy-related protein 12, APG12-like, Ubiquitin-like protein ATG12

SwissProt ID

O94817 (Human), P38316 (Yeast), Q2TBJ5 (Rat), Q3T0W7 (Bovin), Q9CQY1 (Mouse)

Gene ID

852518 (ATG12), 9140, 67526 (Atg12), 361321

Available reactivities

S. cerevisiae (Saccharomyces cerevisiae), Ye (Yeast), Hu (Human), Rt (Rat), Ms (Mouse), Can (Canine), Mky (Monkey), Bov (Bovine), Eq (Equine), Por (Porcine), Ze (Zebrafish)

Available hosts

Rabbit, Mouse, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Paraffin Sections (P)

Background of ATG12 / APG12 antibody

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG12, another member of the autophagy protein family, forms a conjugate with ATG5; this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy. This conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells. ATG12 has also been shown to interact with ATG10 in human embryonic kidney cells in the presence of ATG7. At least two isoforms of ATG12 are known to exist.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ATG12 / APG12 (N-term) antibody

ATG12 / APG12 Goat IgG Aff - Purified Can, Hu, Mky, Ms, Rt ICC/IF, WB
20 µl / €50.00
  SICGEN

ATG12 / APG12 (N-term) antibody

ATG12 / APG12 Goat IgG Aff - Purified Can, Hu, Mky, Ms, Rt ICC/IF, WB
0.6 mg / €320.00
  SICGEN

ATG12 / APG12 (N-term) antibody

  Rabbit Polyclonal ATG12 Antibody  
Western blot analysis of ATG12 in human brain tissue lysate with ATG12 antibody at (A) 0.5, and (B) 1 ug/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ATG12 / APG12 (Center) antibody

  Rabbit Polyclonal ATG12 Antibody  
Western blot analysis of ATG12 in mouse heart tissue lysate with ATG12 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ATG12 / APG12 antibody

  Rabbit Polyclonal Anti-ATG12 Antibody  
WB Suggested Anti-ATG12 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: 721_B cell lysate. ATG12 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells Rabbit IgG Purified Bov, Can, Eq, Hu, Por, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

ATG12 / APG12 antibody

  Rabbit Polyclonal Anti-ATG12 Antibody  
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (ATG12 Antibody) at dilution 1/70, on the right is treated with fusion protein. (Original magnification: ×200) Rabbit IgG Purified Hu E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

ATG12 / APG12 (N-term) antibody

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with Autophagy APG12L Antibody Cat.-No AP32178PU-N (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

ATG12 / APG12 antibody

Figure 1. Immunoblot of APG12 fusion protein. Anti-APG12 antibody generated by immunization with recombinant yeast APG12
was tested by immunoblot against yeast lysates expressing the APG12-GFP fusion protein and other UBL fusion proteins. All UBLs
possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody
against each UBL. Panel A shows total protein staining using ponceau. Panel B shows positions of free GFP or GFP containing
recombinant proteins present in each lysate preparation after reaction with a 1:1,000 dilution of anti-GFP followed by reaction with a 1:15,000 dilution of HRP Donkey-a-Goat IgG MX. Panel C shows specific
reaction with APG12 using a 1:2,000 dilution of IgG fraction of Rabbit-anti-APG12 (Yeast) followed by reaction with a
1:15,000 dilution of HRP Goat-a-Rabbit IgG MX. All primary antibodies were diluted in TTBS buffer supplemented
with 5% non-fat milk and incubated with the membranes overnight at 4° C. Yeast lysate proteins were separated by SDS-PAGE using a
15% gel. This data indicates that anti-APG12 is highly specific and does not cross react with other UBLs. A chemiluminescence
system was used for signal detection (Roche). Other detection systems will yield similar results. Data contributed by M. Malakhov,
Lifesensors Inc., personal communication. Rabbit Purified S. cerevisiae, Ye E, WB
0.5 mg / €510.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

ATG12 - human gene knockout kit via CRISPR

ATG12 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

ATG12 - human gene knockout kit via CRISPR

ATG12 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

ATG12 - human gene knockout kit via CRISPR

ATG12 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

ATG12 - human gene knockout kit via CRISPR

ATG12 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Atg12 - mouse gene knockout kit via CRISPR

Atg12 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Atg12 - mouse gene knockout kit via CRISPR

Atg12 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Atg12 - mouse gene knockout kit via CRISPR

Atg12 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Atg12 - mouse gene knockout kit via CRISPR

Atg12 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

ATG12 overexpression lysate

ATG12 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, ATG12 (mGFP-tagged) - Human ATG12 autophagy related 12 homolog (S. cerevisiae) (ATG12), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, ATG12 (mGFP-tagged) - Human ATG12 autophagy related 12 homolog (S. cerevisiae) (ATG12), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, ATG12 (Myc-DDK tagged) - Human ATG12 autophagy related 12 homolog (S. cerevisiae) (ATG12), transcript variant 1 , 200 uL, >10^7 TU/mL

Lenti ORF particles, ATG12 (Myc-DDK tagged) - Human ATG12 autophagy related 12 homolog (S. cerevisiae) (ATG12), transcript variant 1 , 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Atg12 (GFP-tagged) - Mouse autophagy-related 12 (yeast) (Atg12), 200 uL, >10^7 TU/mL

Lenti ORF particles, Atg12 (GFP-tagged) - Mouse autophagy-related 12 (yeast) (Atg12), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

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