ATG12 / APG12 antibody

Principal name

ATG12 / APG12 antibody

Alternative names for ATG12 / APG12 antibody

APG12L, Autophagy-related protein 12, APG12-like, Ubiquitin-like protein ATG12

SwissProt ID

O94817 (Human), P38316 (Yeast), Q2TBJ5 (Rat), Q3T0W7 (Bovin), Q9CQY1 (Mouse)

Gene ID

852518 (ATG12), 9140, 361321 (Atg12), 67526

Available reactivities

S. cerevisiae (Saccharomyces cerevisiae), Ye (Yeast), Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Mky (Monkey), Bov (Bovine), Eq (Equine), Por (Porcine), Ze (Zebrafish)

Available hosts

Rabbit, Mouse, Sheep, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P), ELISA (detection) (E(detection)), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of ATG12 / APG12 antibody

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG12, another member of the autophagy protein family, forms a conjugate with ATG5; this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy. This conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells. ATG12 has also been shown to interact with ATG10 in human embryonic kidney cells in the presence of ATG7. At least two isoforms of ATG12 are known to exist.

General readings

Mizushima N et al. Mouse Apg16L, a novel WD-repeat protein, targets to the autophagic isolation membrane with the Apg12-Apg5 conjugate. J Cell Sci 116:1679-88 (2003). PubMed PMID: 12665549
Kuma A et al. Formation of the approximately 350-kDa Apg12-Apg5.Apg16 multimeric complex, mediated by Apg16 oligomerization, is essential for autophagy in yeast. J Biol Chem 277:18619-25 (2002). PubMed PMID: 11897782
Suzuki K et al. The pre-autophagosomal structure organized by concerted functions of APG genes is essential for autophagosome formation. EMBO J 20:5971-81 (2001). PubMed PMID: 11689437
Jentsch S & Pyrowolakis G Ubiquitin and its kin: how close are the family ties? Trends Cell Biol 10:335-42 (2000). PubMed PMID: 10884686
Mizushima N et al. A new protein conjugation system in human. The counterpart of the yeast Apg12p conjugation system essential for autophagy. J Biol Chem 273:33889-92 (1998). PubMed PMID: 9852036
Liakopoulos D et al. A novel protein modification pathway related to the ubiquitin system. EMBO J 17:2208-14 (1998). PubMed PMID: 9545234

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ATG12 / APG12 antibody

Confocal microscopy on stained ATG12 using rabbit ATG12 polyclonal antibody ( Cat # PAB0713 ) in paraffin embedded human brian section ( midfrontal cortex , Alzheimer case ) . Rabbit Hu, Rt C, ICC/IF, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

ATG12 / APG12 (N-term) antibody

ATG12 / APG12 Goat IgG Aff - Purified Can, Hu, Mky, Ms, Rt ICC/IF, WB
20 µl / €50.00
  SICGEN

ATG12 / APG12 (N-term) antibody

ATG12 / APG12 Goat IgG Aff - Purified Can, Hu, Mky, Ms, Rt ICC/IF, WB
0.6 mg / €320.00
  SICGEN

ATG12 / APG12 (N-term) antibody

Western blot analysis of ATG12 in human brain tissue lysate with ATG12 antibody at (A) 0.5, and (B) 1 ug/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ATG12 / APG12 (Center) antibody

Western blot analysis of ATG12 in mouse heart tissue lysate with ATG12 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ATG12 / APG12 antibody

WB Suggested Anti-ATG12 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: 721_B cell lysate. ATG12 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells Rabbit IgG Purified Bov, Can, Eq, Hu, Por, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

ATG12 / APG12 (N-term) antibody

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with Autophagy APG12L Antibody Cat.-No AP32178PU-N (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

ATG12 / APG12 antibody

Figure 1. Immunoblot of APG12 fusion protein. Anti-APG12 antibody generated by immunization with recombinant yeast APG12
was tested by immunoblot against yeast lysates expressing the APG12-GFP fusion protein and other UBL fusion proteins. All UBLs
possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody
against each UBL. Panel A shows total protein staining using ponceau. Panel B shows positions of free GFP or GFP containing
recombinant proteins present in each lysate preparation after reaction with a 1:1,000 dilution of anti-GFP followed by reaction with a 1:15,000 dilution of HRP Donkey-a-Goat IgG MX. Panel C shows specific
reaction with APG12 using a 1:2,000 dilution of IgG fraction of Rabbit-anti-APG12 (Yeast) followed by reaction with a
1:15,000 dilution of HRP Goat-a-Rabbit IgG MX. All primary antibodies were diluted in TTBS buffer supplemented
with 5% non-fat milk and incubated with the membranes overnight at 4° C. Yeast lysate proteins were separated by SDS-PAGE using a
15% gel. This data indicates that anti-APG12 is highly specific and does not cross react with other UBLs. A chemiluminescence
system was used for signal detection (Roche). Other detection systems will yield similar results. Data contributed by M. Malakhov,
Lifesensors Inc., personal communication. Rabbit Purified S. cerevisiae, Ye E, WB
0.5 mg / €440.00
  Acris Antibodies GmbH

ATG12 / APG12 antibody

Western Blot analysis of ATG12 expression in transfected 293T cell line (H00009140-T01) by ATG12 MaxPab polyclonal antibody.

Lane 1: ATG12 transfected lysate(20.57 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

ATG12 / APG12 antibody

Detection limit for recombinant GST tagged ATG12 is approximately 1ng/ml as a capture antibody. Mouse IgG1 2H8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ATG12 / APG12 antibody

ATG12 / APG12 Mouse IgG1 1F9 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ATG12 / APG12 antibody

Western blot analysis of Atg12 expression in NIH/3T3 cell lysate ( Lane 1 ) and rat kidney tissue lysate ( Lane 2 ) . Rabbit Hu, Ms, Rt WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

ATG12 / APG12 antibody

Immunoblot of APG12 fusion protein.
ATG12 polyclonal antibody ( Cat # PAB11358 ) generated by immunization with recombinant yeast ATG12 was tested by immunoblot against yeast lysates expressing the ATG12-GFP fusion protein and other UBL fusion proteins.
All UBLs possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody against each UBL.
Panel A shows total protein staining using ponceau.
Panel B shows positions of free GFP or GFP containing recombinant proteins present in each lysate preparation after reaction with a 1:1,000 dilution of anti-GFP followed by reaction with a 1:15,000 dilution of HRP Donkey-a-Goat IgG MX.
Panel C shows specific reaction with ATG12 using a 1:2,000 dilution of ATG12 polyclonal antibody ( Cat # PAB11358 ) followed by reaction with a 1:15,000 dilution of HRP Goat-a-Rabbit IgG MX.
All primary antibodies were diluted in TTBS buffer supplemented with 5% non-fat milk and incubated with the membranes overnight at 4°C.
Yeast lysate proteins were separated by SDS-PAGE using a 15% gel. This data indicates that anti-ATG12 is highly specific and does not cross react with other UBLs.
A chemiluminescence system was used for signal detection ( Roche ) .
Data contributed by M. Malakhov, Lifesensors Inc., personal communication. Rabbit Ye E, WB
0.5 mg / €560.00
  Abnova Taiwan Corp.

ATG12 / APG12 antibody

Immunohistochemistry of ATG12 in human brain tissue with ATG12 polyclonal antibody ( Cat # PAB13321 ) at 2.5 µg/ml. Rabbit Hu, Ms, Rt P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

ATG12 / APG12 antibody

Western blot analysis of ATG12 in mouse heart tissue lysate with ATG12 polyclonal antibody ( Cat # PAB13322 ) at 1 µg/ml in ( A ) the absence and ( B ) the presence of blocking peptide. Rabbit Hu, Ms, Rt WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

ATG12 / APG12 antibody

ATG12 / APG12 Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

ATG12 / APG12 (C-term) antibody

Rat Kidney; WB Suggested Anti-Atg12 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Rat Kidney; Atg12 antibody - C-terminal region (ARP59030_P050) in Rat Kidney cells using Western Blot Rabbit Aff - Purified Hu, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

ATG12 / APG12 antibody

Western Blot: ATG12 Antibody [NB600-603] - Immunoblot of APG12 fusion protein. Anti-APG12 antibody generated by immunization with recombinant yeast APG12 was tested by immunoblot against yeast lysates expressing the APG12-GFP fusion protein and other UBL fusion proteins. All UBLs possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody against each UBL. Panel A shows total protein staining using ponceau. Panel B shows positions of free GFP or GFP containing recombinant proteins present in each lysate preparation after reaction with a 1:1,000 dilution of anti-GFP  followed by reaction with a 1:15,000 dilution of HRP Donkey-a-Goat IgG MX. Panel C shows specific reaction with APG12 using a 1:2,000 dilution of IgG fraction of Rabbit-anti-APG12 (Yeast) followed by reaction with a 1:15,000 dilution of HRP Goat-a-Rabbit IgG MX. All primary antibodies were diluted in TTBS buffer supplemented with 5% non-fat milk and incubated with the membranes overnight at 4° C. Yeast lysate proteins were separated by SDS-PAGE using a 15% gel. This data indicates that anti-APG12 is highly specific and does not cross react with other UBLs. A chemiluminescence  system was used for signal detection (Roche). Other detection systems will yield similar results. Rabbit Ig Fraction Ye E, WB
0.25 mg / €320.00
  Novus Biologicals Inc.

ATG12 / APG12 antibody

Western Blot: ATG12 Antibody [NBP1-45349] - Western blot analysis of Apg12 in 3T3 cell lysate (Lane 1) and rat kidney tissue lysate (Lane 2). Rabbit IgG Aff - Purified Hu, Ms, Rt WB
0.1 mg / €440.00
  Novus Biologicals Inc.

ATG12 / APG12 antibody

Immunohistochemistry-Paraffin: ATG12 Antibody [NBP1-76860] - Human brain tissue with ATG12 antibody at 2.5 ug/mL. _x000d_ Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

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