ATG13 antibody

Principal name

ATG13 antibody

Alternative names for ATG13 antibody

KIAA0652, Autophagy-related protein 13

SwissProt ID

O75143 (Human), Q08DY8 (Bovin), Q91YI1 (Mouse)

Gene ID

9776 (ATG13), 1467726 (Q9Z6Q0)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunoprecipitation (IP), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)

Background of ATG13 antibody

The autophagy-related protein 13 (Atg13) plays an important role in the formation of autophagosomes. Autophagosomes are formed in response to nutrient deprivation and function as the transport vesicles for organelles, proteins, and protein complexes targeted for lysosomes that digest these cargos to produce energy and nutrients. Atg13 is activated by the mTOR pathway and forms a complex with the FIP200 protein. This complex is involved in enhancing the activity of the ULK1 kinase which is required for the formation of autophagosomes. Atg13/FIP200 faciliates the localization of ULK1 to pre-autophagosomes, and subsequently stabilizes ULK1. Autophagy is an important process in development, growth, and cell differentiation, and disruption of this process may contribute to cancer, aging, and neurodegenerative diseases.

General readings

Hosokawa, N., et al. Autophagy 5(7):973-979(2009)
Mercer, C.A., et al. Autophagy 5(5):649-662(2009)
Ganley, I.G., et al. J. Biol. Chem. 284(18):12297-12305(2009)
Jung, C.H., et al. Mol. Biol. Cell 20(7):1992-2003(2009)
Hosokawa, N., et al. Mol. Biol. Cell 20(7):1981-1991(2009)

13 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ATG13 antibody

Western blot analysis of ATG13 in rat heart tissue lysate with ATG13 antibody at 1 µg/mL in (A) the absence and (B) the presence of blocking peptide. Rabbit Purified Hu, Ms, Rt E, P, WB
0.1 mg / €345.00
  OriGene Technologies, Inc.

ATG13 antibody

WB using Anti-ATG13 antibody shows detection of ATG13 in 293T cells engineered to coexpress Ulk1 and Atg13 (Ulk1 + Atg13), right lane, but not in the left lane in which was loaded kinase-dead hypophosphorylated Ulk1-K46A mutant + ATG13. Detection is demonstrated at approximately 57 kDa. primary antibody was used at 1µg/mL overnight at 4°C. After washing, the membrane was probed with Goat Anti-Rabbit HRP secondary 1:5000. Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ATG13 (C-term) antibody

Western blot analysis of ATG13 in rat heart tissue lysate with ATG13 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ATG13 antibody

Predicted band size: 57 kDa . Positive control: Human brain tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ATG13 antibody

Western blot analysis of extracts of various cell lines, using ATG13 antibody. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ATG13 antibody

WB Suggested Anti-ATG13 Antibody; Titration: 1.0 ug/ml; Positive Control: 293T Whole Cell There is BioGPS gene expression data showing that ATG13 is expressed in HEK293T Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

ATG13 control peptide

Western blot analysis of ATG13 in rat heart tissue lysate with ATG13 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
50 µg / €160.00
  Acris Antibodies GmbH

ATG13 (C-term) antibody

Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

ATG13 (N-term) antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma reacted with KIAA0652 Antibody (N-term) Cat.-No AP52338PU-N, which was peroxidase conjugated to the secondary antibody and followed byDAB staining. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

ATG13 antibody

Immunohistochemistry-Paraffin: ATG13 Antibody [NBP1-87858] - Immunohistochemical staining of human kidney shows strong membranous positivity in tubular cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ATG13

ATG13 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

ATG13

ATG13 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

ATG13 overexpression lysate

ATG13 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

13 Item(s)

per page
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