ATM antibody

Principal name

ATM antibody

Alternative names for ATM antibody

Serine-protein kinase ATM, Ataxia telangiectasia mutated, A-T mutated, ATDC, TEL1, TELO1

SwissProt ID

Q13315 (Human), Q62388 (Mouse), Q6PQD5 (Pig)

Gene ID

472 (ATM), 5144724 (mpg), 11920 (Atm)

Available reactivities

Hu (Human), Mky (Monkey), Ms (Mouse), Rt (Rat), Hst (Hamster), Por (Porcine)

Available hosts

Mouse, Rabbit, Goat, Chicken, Sheep

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), PLA/Proximity ligation assay (PLA), Enzyme Immunoassay (E), Frozen Sections (C), Dot blot (Dot), Flow Cytometry (F)

Background of ATM antibody

ATM, the gene mutated in the hereditary disease ataxia-telangiectasia, codes for a protein kinase that acts as a master regulator of cellular responses to DNA double-strand breaks. ATM is normally inactive and the question of how it is activated in the event of DNA damage (due to ionizing radiation for instance) is central to understanding its function. ATM protein is now shown to be present in undamaged cells as an inactive dimer. Low doses of ionizing radiation, which induce only a few DNA breaks, activate at least half of the total ATM protein present, possibly in response to changes in chromatin structure. The ATM gene encodes a 370-kDa protein that belongs to the phosphoinositide 3-kinase (PI(3)K) superfamily, but which phosphorylates proteins rather than lipids. The 350-amino-acid kinase domain at the carboxy terminus of this large protein is the only segment of ATM with an assigned function. Exposure of cells to IR triggers ATM kinase activity, and this function is required for arrests in G1, S and G2 phases of the cell cycle. Several substrates of the ATM kinase participate in these IR-induced cell-cycle arrests. These include p53, Mdm2 and Chk2 in the G1 checkpoint; Nbs1, Brca1, FancD2 and SMC1 in the transient IR-induced S-phase arrest; and Brca1 and hRad17 in the G2/M checkpoint. 
Structure: 3056 amino acids, 350 kD.
Distribution: Primarily nuclear, also found in endocytic vesicles.
Function: Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis, and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor.

General readings

Rogoff, H., et al. Apoptosis Associated with Derefulated E2F Activity Is Dependent on E2F1 and Atm/Nbs1/Chk2. Mol. and Cell. Biol. 24(7): 2968-2977 (2004) 1. Out, R., et al. Adenovirus-mediated hepatic overexpression of scavenger receptor class B tyoe I accelerates chylomicron metabolism in C57BL/6J mice. J. Lipid Res. 46: 1172-1181 (2005) [Western blot, Mouse]
2. Cuadrado, M., et al. ATM regulates ATR chromatin loading in response to DNA double-strand breaks. JEM. 203(2): 297-303 (2006)
3. Gaspar, M., and Shenk, T. Human cytomegalovirus inhibits a DNA damage response by mislocalizing checkpoint proteins. PNAS. 103(8): 2821-2826 (2006)
4. Hiyama, T., et al. Haploinsufficiency of the Mus81-Eme1 endonuclease activates the intra-S-phase and G2/M checkpoints and promotes rereplication in human cells. Nucl. Acids Res. 34(3): 880-892 (2006)
5. Ho, GPH., et al. Phosphorylation of FANCD2 on Two Novel Sites Is Required for Mitomycin C Resistance. Mol. Cell Biol. 26(18): 7005-7015 (2006)
6. Hovest, M. G., et al. Senescence of Human Fibroblasts after Psoralen Photoactivation Is Mediated by ATR Kinase and Persistent DNA Damage Foci at Telomeres. Mol. Biol. Cell. 17(4): 1758-1767 (2006)
7. Lovejoy, C. A., et al. DDB1 Maintains Genome Integrity through Regulation of Cdt1. Mol. Cell Biol. 26(21): 7977-7990 (2006)
8. Yang, S., et al. Promyelocytic Leukemia Activates Chk2 by Mediating Chk2 Autophosphorylation. JBC. 281(36): 26645-26654 (2006)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ATM antibody

Western Blot: ATM Antibody [TA336258] - Detection of ATM in HeLa nuclear extract using TA336258. (30 sec. exposure) Rabbit Purified Hu, Ms C, ICC/IF, IP, P, WB
50 µl / €325.00
  OriGene Technologies, Inc.

ATM antibody

Immunohistochemistry-Paraffin: ATM Antibody (2C1) [NB100-309] - Human Kidney (formalin-fixed, paraffin-embedded) stained with ATM antibody at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Mouse IgG1 2C1 Purified Hu, Mky, Ms, Rt ICC/IF, IP, P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

ATM antibody

Western blot analysis of ATM in Daudi whole cell lysate with ATM antibody at (A) 1 and (B) 2 ug/ml. Rabbit IgG Aff - Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ATM antibody

Western Blot: ATM Kinase Phosphospecific Antibody (10H11.E12) - Western Blot analysis of ATM-kinase. Sample: Irradiated or peroxidated human fibroblasts. Mouse IgG1 10H11.E12 Purified Hu, Ms, Rt C, ICC/IF, IP, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

ATM antibody

Western blot analysis of extracts from 293T cells using ATM Antibody Rabbit IgG Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ATM antibody

Kidney: Formalin-Fixed Paraffin Embedded (FFPE) Mouse IgG1 3E8 Purified Hu IP, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

ATM antibody

Western blot analysis using ATM antibody Cat.-No AM06585SU-N against HEK293 (1) and ATM(AA: 2705-2820)-hIgGFc transfected HEK293 (2) cell lysate. Mouse IgG1 5C5 Ascites Hu E, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

ATM antibody

Western blot analysis of ATM expression in Lu99A (1) and T24 (2) using AM26642AF-N. Mouse IgG1 4H1 Azide Free Hu WB
0.1 mg / €350.00
  Acris Antibodies GmbH

ATM antibody

Human prostate adenocarcinoma stained with anti-ATM Antibody Cat.-No AM33235PU (Clone 224). Rabbit IgG SP224 Purified Hu, Ms, Por F, P
0.5 ml / €520.00
  Acris Antibodies GmbH

ATM antibody

Human prostate adenocarcinoma stained with anti-ATM Antibody Cat.-No AM33235PU (Clone 224). Rabbit IgG SP224 Purified Hu, Ms, Por F, P
1 ml / €730.00
  Acris Antibodies GmbH

ATM antibody

Human prostate adenocarcinoma stained with anti-ATM Antibody Cat.-No AM33235PU (Clone 224). Rabbit IgG SP224 Purified Hu, Ms, Por F, P
0.1 ml / €300.00
  Acris Antibodies GmbH

ATM pSer1981 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATM (phospho-Ser1981) antibody (AP02410PU-N and AP024PU-S. Rabbit Aff - Purified Hu P, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

ATM pSer1981 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATM (phospho-Ser1981) antibody (AP02410PU-N and AP024PU-S. Rabbit Aff - Purified Hu P, WB
50 µl / €240.00
  Acris Antibodies GmbH

ATM antibody

Liver: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu P, WB
50 µg / €470.00
  Acris Antibodies GmbH

ATM (1979-1983) antibody

Immunofluorescence staining of methanol-fixed HeLa cells using ATM Antibody (AP08052PU, Red). Rabbit Aff - Purified Hu, Ms ICC/IF, WB
0.1 ml / €270.00
  Acris Antibodies GmbH

ATM antibody

Immunofluorescence staining of methanol-fixed HeLa cells using ATM Antibody (AP08052PU, Red). Rabbit Aff - Purified Hu, Ms ICC/IF, WB
50 µl / €230.00
  Acris Antibodies GmbH

ATM pSer1981 antibody

Lane 2 shows anti-ATM pS1981 polyclonal antibody detects ATM in Hydrogen Peroxide Stimulated Human Derived MCF-7 Whole Cell Lysate. Lane 1 shows a lysate from untreated MCF-7 cell lysate. The band corresponding to ATM (370 kDa) is indicated by an arrow. After blocking, the membrane was probed with a 1:1000 dilution of the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:5,000 dilution of IRDye(TM)800 conjugated Donkey anti-Rabbit IgG for 1h at room temperature. LICOR's Odyssey(R) Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results. Sheep IgG Aff - Purified Hu E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

ATM pSer1981 antibody

Lane 2 shows anti-ATM pS1981 polyclonal antibody detects ATM in Hydrogen Peroxide Stimulated Human Derived MCF-7 Whole Cell Lysate. Lane 1 shows a lysate from untreated MCF-7 cell lysate. The band corresponding to ATM (370 kDa) is indicated by an arrow. After blocking, the membrane was probed with a 1:1000 dilution of the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:5,000 dilution of IRDye(TM)800 conjugated Donkey anti-Rabbit IgG for 1h at room temperature. LICOR's Odyssey(R) Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results. Sheep IgG Aff - Purified Hu E, WB
25 µl / €190.00
  Acris Antibodies GmbH

ATM pSer1981 antibody

Dot blot analysis of anti-Phospho-ATM-pS1981 Antibody (Cat.#AP12919PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. Rabbit Ig Aff - Purified Hu
0.4 ml / €370.00
  Acris Antibodies GmbH

ATM pSer1981 antibody

Immunohistochemistry (IHC) analyzes of p-ATM antibody (Cat.-No.: AP20875PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu P
0.1 mg / €350.00
  Acris Antibodies GmbH

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