ATP synthase subunit beta antibody

Principal name

ATP synthase subunit beta antibody

Alternative names for ATP synthase subunit beta antibody

F1F0 ATP synthase, Complex V, ATP5B, ATPMB, ATPSB, ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide, ATP Synthase subunit F1F0-beta

SwissProt ID

P06576 (Human), P10719 (Rat), P56480 (Mouse)

Gene ID

506 (ATP5B), 11947 (Atp5b), 171374

Available reactivities

Bac (Bacteria), Arabidopsis, Maize, Pl (Plant), Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Por (Porcine), Rb (Rabbit), Rt (Rat), Chk (Chicken), Ze (Zebrafish), African clawed frog, Mky (Monkey)

Available hosts

Chicken, Rabbit, Mouse, Goat

Available applications

Frozen Sections (C), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Paraffin Sections (P), Flow Cytometry (F), Enzyme Immunoassay (E)

Background of ATP synthase subunit beta antibody

ATP5B is a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). ATP5B is the beta subunit of the catalytic core.This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Vantourout,P., (2008) Mol. Immunol. 45 (2), 485-492

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ATP synthase subunit beta antibody

  anti-ATP5B mouse monoclonal antibody, clone OTI1F7 (formerly 1F7)  
Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-ATP5Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500832, Dilution 1:50) Mouse IgG1 OTI1F7 Purified Can, Hu, Mky F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ATP synthase subunit beta antibody

  anti-ATP5B mouse monoclonal antibody, clone OTI4E5 (formerly 4E5)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-ATP5Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500833, Dilution 1:50) Mouse IgG2a OTI4E5 Purified Can, Hu, Mky, Rt F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ATP synthase subunit beta antibody

  anti-ATP5B mouse monoclonal antibody, clone OTI6C4 (formerly 6C4)  
Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-ATP5Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500834, Dilution 1:50) Mouse IgG1 OTI6C4 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ATP synthase subunit beta antibody

  anti-ATP5B mouse monoclonal antibody, clone OTI9E3 (formerly 9E3)  
Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-ATP5B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500835, Dilution 1:50) Mouse IgG1 OTI9E3 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ATP synthase subunit beta antibody

  anti-ATP5B mouse monoclonal antibody, clone OTI3H8 (formerly 3H8)  
Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-ATP5B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500836, Dilution 1:50) Mouse IgG1 OTI3H8 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ATP synthase subunit beta antibody

  anti-ATP5B mouse monoclonal antibody, clone OTI1B10 (formerly 1B10)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-ATP5B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500837, Dilution 1:50) Mouse IgG1 OTI1B10 Purified Can, Hu, Mky, Rt F, ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

ATP synthase subunit beta antibody

  anti-ATP5B mouse monoclonal antibody, clone OTI7F4 (formerly 7F4)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-ATP5B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500849, Dilution 1:50) Mouse IgG1 OTI7F4 Purified Can, Hu, Mky F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ATP synthase subunit beta antibody

  anti-ATP5B mouse monoclonal antibody, clone OTI6B11 (formerly 6B11)  
Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-ATP5B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500850, Dilution 1:50) Mouse IgG1 OTI6B11 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ATP synthase subunit beta antibody

  anti-ATP5B mouse monoclonal antibody, clone OTI5G2 (formerly 5G2)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-ATP5B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500851, Dilution 1:50) Mouse IgG1 OTI5G2 Purified Can, Hu, Mky, Rt F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ATP synthase subunit beta antibody

  ATP5B (aa15162) Goat Polyclonal Antibody  
TA329077 (0.3 ug/ml) staining of Human (A), Mouse (B) and Rat (C) Heart lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ATP synthase subunit beta antibody

  Rabbit anti-ATP5B Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using ATP5B antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ATP synthase subunit beta antibody

  anti-ATP5B mouse monoclonal antibody, clone OTI6C10 (formerly 6C10)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP5B  (RC201638, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP5B . Mouse IgG2b OTI6C10 Purified Can, Hu, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ATP synthase subunit beta (Center) antibody

Formalin-fixed and paraffin-embedded human brain tissue reacted with ATP5B Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Hu F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

Atp5b - mouse gene knockout kit via CRISPR

Atp5b - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Atp5b - mouse gene knockout kit via CRISPR

Atp5b - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Atp5b - mouse gene knockout kit via CRISPR

Atp5b - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Atp5b - mouse gene knockout kit via CRISPR

Atp5b - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ATP synthase subunit beta

  Recombinant protein of human ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide (ATP5B), nuclear gene encoding mitochondrial protein  
ATP synthase subunit beta Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Atp5b (GFP-tagged) - Mouse ATP synthase, H+ transporting mitochondrial F1 complex, beta subunit (Atp5b), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL

Lenti ORF particles, Atp5b (GFP-tagged) - Mouse ATP synthase, H+ transporting mitochondrial F1 complex, beta subunit (Atp5b), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL  
200 µl / €1,160.00
  OriGene Technologies, Inc.

Lenti ORF particles, Atp5b (Myc-DDK-tagged) - Mouse ATP synthase, H+ transporting mitochondrial F1 complex, beta subunit (Atp5b), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL

Lenti ORF particles, Atp5b (Myc-DDK-tagged) - Mouse ATP synthase, H+ transporting mitochondrial F1 complex, beta subunit (Atp5b), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL  
200 µl / €1,040.00
  OriGene Technologies, Inc.

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