ATP2A1 / SERCA1 antibody

Principal name

ATP2A1 / SERCA1 antibody

Alternative names for ATP2A1 / SERCA1 antibody

Calcium pump 1 ATPase, Sarcoplasmic/endoplasmic reticulum calcium ATPase 1, Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform, SR Ca(2+)-ATPase 1, Endoplasmic reticulum class 1/2 Ca(2+) ATPase

SwissProt ID

O14983 (Human), P04191 (Rabit), P13585 (Chick), Q0VCY0 (Bovin), Q64578 (Rat), Q8R429 (Mouse)

Gene ID

11937 (Atp2a1), 487 (ATP2A1), 116601

Available reactivities

Can (Canine), GP (Guinea Pig), Ms (Mouse), Rb (Rabbit), Rt (Rat), Hu (Human), Bov (Bovine)

Available hosts

Mouse, Rabbit

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Frozen Sections (C), Immunoprecipitation (IP)

Background of ATP2A1 / SERCA1 antibody

Three Ca2+ ATPases have been described in mammalian cells. They are located in the plasma membrane, endoplasmic reticulum or the Golgi apparatus. SERCA pumps are located in both the endoplasmic reticulum and in the Golgi membranes. They are known to transport two Ca2+ molecules per hydrolysis of one ATP. Their structure includes ten transmembrane domains and their main role is to remove cytoplasmic Ca2+ ions in order to promote muscle relaxation. In mammals three genes encode three SERCA pumps. Each transcript undergoes tissue-dependent altertive splicing. SERCA1a and 1b are expressed in adult and neotal skeletal muscle respectively. SERCA2a is also expressed in skeletal muscle, while SERCA2b is ubiquitously expressed. SERCA3 is expressed in a limited number of non-muscle cells. Although all SERCAs are regulated, SERCA2b undergoes extensive regulation at the protein level, such as protein-protein interaction, phosphorylation and glycosylation. The expression of SERCA1 in mice is essential whereas in human its absence is tolerated but is the cause of Brody myopathy. Malfunction of SERCAs is also observed in heart disease and different forms of cancer.

General readings

This product has been used in:Zhou D et al. Small, membrane-bound, alternatively spliced forms of ankyrin 1 associated with the sarcoplasmic reticulum of mammalian skeletal muscle. J Cell Biol 136:621-31 (1997). PubMed PMID: 9024692
Hu P et al. Transcriptional regulation of phospholamban gene and translational regulation of SERCA2 gene produces coordinate expression of these two sarcoplasmic reticulum proteins during skeletal muscle phenotype switching. J Biol Chem 270:11619-22 (1995). PubMed PMID: 7744801
Molnar E et al. Immunological relatedness of the sarcoplasmic reticulum Ca(2+)-ATPase and the Na+,K(+)-ATPase. Biochim Biophys Acta 1103:281-95 (1992). PubMed PMID: 1371934
Ohlendieck K et al. Analysis of excitation-contraction-coupling components in chronically stimulated canine skeletal muscle. Eur J Biochem 202:739-47 (1991). PubMed PMID: 1662614
Molnar E et al. The binding of monoclonal and polyclonal antibodies to the Ca2(+)-ATPase of sarcoplasmic reticulum: effects on interactions between ATPase molecules. Biochim Biophys Acta 1023:147-67 (1990). PubMed PMID: 1691656
Briggs FN et al. Ca-ATPase isozyme expression in sarcoplasmic reticulum is altered by chronic stimulation of skeletal muscle. FEBS Lett 259:269-72 (1990). PubMed PMID: 2136731
Jorgensen AO et al. A monoclonal antibody to the Ca2+-ATPase of cardiac sarcoplasmic reticulum cross-reacts with slow type I but not with fast type II canine skeletal muscle fibers: an immunocytochemical and immunochemical study. Cell Motil Cytoskeleton 9:164-74 (1988). PubMed PMID: 2965994

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ATP2A1 / SERCA1 (522-613) antibody

Human Skeletal muscle: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 1B11 Purified Hu E, ICC/IF, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

ATP2A1 / SERCA1 antibody

Immunoperoxidase of monoclonal antibody to ATP2A1 on formalin-fixed paraffin-embedded human cerebral cortex. [antibody concentration 3 ug/ml] Mouse IgG1 1B11 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ATP2A1 / SERCA1 antibody

Western blot analysis of extracts from NIH/3T3 cells, A549 cells and HT-29 cells, using ATP2A1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ATP2A1 / SERCA1 antibody

Western blot analysis of mouse C2C12 myoblast cell line lysate (lanes 1 and 3) and rat skeletal muscle membranes (lanes 2 and 4): 1, 2. Anti-SERCA1 antibody, (1:200). 3, 4. Anti-SERCA1 antibody, preincubated with the control peptide antigen. Rabbit Purified Ms, Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

ATP2A1 / SERCA1 antibody

ATP2A1 / SERCA1 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

ATP2A1 / SERCA1 antibody

ATP2A1 / SERCA1 Mouse IgG1 6F8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ATP2A1 / SERCA1 antibody

ATP2A1 / SERCA1 Mouse IgG1 2C7 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ATP2A1 / SERCA1 antibody

ATP2A1 / SERCA1 Mouse IgG1 5H8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ATP2A1 / SERCA1 antibody

Immunoperoxidase of monoclonal antibody to ATP2A1 on formalin-fixed paraffin-embedded human skeletal muscle. [antibody concentration 3 ug/ml] Mouse IgG3 4B8 Purified Hu E, P, WB
50 µg / €370.00
  Abnova Taiwan Corp.

ATP2A1 / SERCA1 antibody

ATP2A1 / SERCA1 Mouse IgG3 4B8 Ascites Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

ATP2A1 / SERCA1 antibody

ATP2A1 / SERCA1 Mouse IgG3 3H4 Ascites Hu E
0.2 ml / €370.00
  Abnova Taiwan Corp.

ATP2A1 / SERCA1 antibody

ATP2A1 / SERCA1 Mouse IgG3 2C9 Ascites Hu E
0.2 ml / €370.00
  Abnova Taiwan Corp.

ATP2A1 / SERCA1 antibody

ATP2A1 / SERCA1 Mouse IgG1 5G4 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ATP2A1 / SERCA1 antibody

Western blot analysis of extracts from HT-29/A549/NIH-3T3 cells, using ATP2A1 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

ATP2A1 / SERCA1 antibody

Western blot analysis of extracts from HT-29/A549/NIH-3T3 cells, using ATP2A1 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

ATP2A1 / SERCA1 antibody

Rhesus macaque spinal cord; . Sample Type :. Rhesus macaque spinal cord. Primary Antibody Dilution :. 1:300. Secondary Antibody :. Donkey anti Rabbit 488. Secondary Antibody Dilution :. 1:500. Color/Signal Descriptions :. Green: ATP2A1. Gene Name :. ATP2A1. Submitted by :. Timur Mavlyutov, Ph. D., Department of Pharmacology, University of Wisconsin Medical School, 1300 University Avenue, Madison, WI 53706; ATP2A1 antibody - N-terminal region (ARP42179_P050) in Rhesus macaque spinal cord cells using Immunohistochemistry Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

ATP2A1 / SERCA1 antibody

ATP2A1 / SERCA1 Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €290.00
  Boster Immunoleader

ATP2A1 / SERCA1 antibody

Immunohistochemical analysis of SERCA1 staining in human muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Ms, Rb, Rt IP, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

ATP2A1 / SERCA1 antibody

Immunohistochemical analysis of SERCA1 staining in human muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Ms, Rb, Rt IP, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

ATP2A1 / SERCA1 antibody

Immunohistochemical analysis of SERCA1 staining in human muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Ms, Rb, Rt IP, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

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