ATP2A2 / SERCA2 antibody

Principal name

ATP2A2 / SERCA2 antibody

Alternative names for ATP2A2 / SERCA2 antibody

Calcium pump 2 ATPase, ATP2B, Sarcoplasmic/endoplasmic reticulum calcium ATPase 2, Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform, SR Ca(2+)-ATPase 2, Endoplasmic reticulum class 1/2 Ca(2+) ATPase

SwissProt ID

O46674 (Canfa), O55143 (Mouse), P11507 (Rat), P11607 (Pig), P16615 (Human), P20647 (Rabit), Q00779 (Felca), Q03669 (Chick)

Gene ID

488 (ATP2A2), 29693 (Atp2a2)

Available reactivities

Hu (Human), Por (Porcine), Rb (Rabbit), Rt (Rat), Bov (Bovine), Can (Canine), GP (Guinea Pig), Ms (Mouse), Dros (Drosophila), Ze (Zebrafish), Mky (Monkey)

Available hosts

Rabbit, Mouse, Goat

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Frozen Sections (C), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Neutralisation (NEUT), Whole mount Immunohistochemistry (IHC-wm)

Background of ATP2A2 / SERCA2 antibody

Three Ca2+ ATPases have been described in mammalian cells. They are located in the plasma membrane, endoplasmic reticulum or the Golgi apparatus. SERCA pumps are located in both the endoplasmic reticulum and in the Golgi membranes. They are known to transport two Ca2+ molecules per hydrolysis of one ATP. Their structure includes ten transmembrane domains and their main role is to remove cytoplasmic Ca2+ ions in order to promote muscle relaxation. In mammals three genes encode three SERCA pumps. Each transcript undergoes tissue-dependent altertive splicing. SERCA1a and 1b are expressed in adult and neotal skeletal muscle respectively. SERCA2a is also expressed in skeletal muscle, while SERCA2b is ubiquitously expressed. SERCA3 is expressed in a limited number of non-muscle cells. Although all SERCAs are regulated, SERCA2b undergoes extensive regulation at the protein level, such as protein-protein interaction, phosphorylation and glycosylation. SERCA2 catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into the sarcoplasmic reticulum lumen, and is involved in regulation of the contraction/relaxation cycle. Mutations in this gene cause Darier-White disease, also known as keratosis follicularis, an autosomal domint skin disorder characterized by loss of adhesion between epidermal cells and abnormal keratinization.

General readings

This product has been used in:Poch E et al. Functional characterization of alternatively spliced human SERCA3 transcripts. Am J Physiol 275:C1449-58 (1998). PubMed PMID: 9843705
Hu P et al. Transcriptional regulation of phospholamban gene and translational regulation of SERCA2 gene produces coordinate expression of these two sarcoplasmic reticulum proteins during skeletal muscle phenotype switching. J Biol Chem 270:11619-22 (1995). PubMed PMID: 7744801
Sharp AH et al. Differential immunohistochemical localization of inositol 1,4,5-trisphosphate- and ryanodine-sensitive Ca2+ release channels in rat brain. J Neurosci 13:3051-63 (1993). PubMed PMID: 8392539
Molnar E et al. Immunological relatedness of the sarcoplasmic reticulum Ca(2+)-ATPase and the Na+,K(+)-ATPase. Biochim Biophys Acta 1103:281-95 (1992). PubMed PMID: 1371934
Plessers L et al. A study of the organellar Ca2(+)-transport ATPase isozymes in pig cerebellar Purkinje neurons. J Neurosci 11:650-6 (1991). PubMed PMID: 1825845
Ohlendieck K et al. Dystrophin-glycoprotein complex is highly enriched in isolated skeletal muscle sarcolemma. J Cell Biol 112:135-48 (1991). PubMed PMID: 1986002
Spencer GG et al. Expression of isoforms of internal Ca2+ pump in cardiac, smooth muscle and non-muscle tissues. Biochim Biophys Acta 1063:15-20 (1991). PubMed PMID: 1826611
Ohlendieck K et al. Analysis of excitation-contraction-coupling components in chronically stimulated canine skeletal muscle. Eur J Biochem 202:739-47 (1991). PubMed PMID: 1662614
Molnar E et al. The binding of monoclonal and polyclonal antibodies to the Ca2(+)-ATPase of sarcoplasmic reticulum: effects on interactions between ATPase molecules. Biochim Biophys Acta 1023:147-67 (1990). PubMed PMID: 1691656
Movsesian MA et al. Identification and characterization of proteins in sarcoplasmic reticulum from normal and failing human left ventricles. J Mol Cell Cardiol 22:1477-85 (1990). PubMed PMID: 2089160
Briggs FN et al. Ca-ATPase isozyme expression in sarcoplasmic reticulum is altered by chronic stimulation of skeletal muscle. FEBS Lett 259:269-72 (1990). PubMed PMID: 2136731
Jorgensen AO et al. A monoclonal antibody to the Ca2+-ATPase of cardiac sarcoplasmic reticulum cross-reacts with slow type I but not with fast type II canine skeletal muscle fibers: an immunocytochemical and immunochemical study. Cell Motil Cytoskeleton 9:164-74 (1988). PubMed PMID: 2965994

13 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ATP2A2 / SERCA2 antibody

Predicted band size: 115 kDa . Positive control: Heart tissue lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ATP2A2 / SERCA2 antibody

TA326319 (0.1µg/ml) staining of Human (A), Mouse (B) and Rat (C) Heart lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Por, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ATP2A2 / SERCA2 antibody

Western blot analysis of rat heart membranes (lanes 1 and 5), mouse heart membranes (lanes 2 and 6), rat PC12 pheochromocytoma cell line lysate (lanes 3 and 7) and human Jurkat T cell leukemia cell line lysate (lanes 4 and 8): 1- 4. Anti-SERCA2 antibody, (1:200). 5- 8. Anti-SERCA2 antibody, preincubated with the control peptide antigen. Rabbit Purified Hu, Ms, Rt ICC/IF, WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

ATP2A2 / SERCA2 antibody

Rhesus macaque spinal cord; . Sample Type :. Rhesus macaque spinal cord. Primary Antibody Dilution :. 1:300. Secondary Antibody :. Donkey anti Rabbit 488. Secondary Antibody Dilution :. 1:500. Color/Signal Descriptions :. Green: ATP2A2. Gene Name :. APLP2. Submitted by :. Timur Mavlyutov, Ph. D., Department of Pharmacology, University of Wisconsin Medical School, 1300 University Avenue, Madison, WI 53706; ATP2A2 antibody - C-terminal region (ARP45159_P050) in Rhesus macaque spinal cord cells using Immunohistochemistry Rabbit Aff - Purified Can, Dros, Hu, Ms, Por, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

ATP2A2 / SERCA2 antibody

ATP2A2 / SERCA2 Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €290.00
  Boster Immunoleader

ATP2A2 / SERCA2 antibody

Western blot analysis of SERCA2 expression in THP1 (A); NIH3T3 (B); PC12 (C) whole cell lysates. Rabbit Aff - Purified Hu, Mky, Ms, Rb, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

ATP2A2 / SERCA2 antibody

Western blot analysis of SERCA2 expression in THP1 (A); NIH3T3 (B); PC12 (C) whole cell lysates. Rabbit Aff - Purified Hu, Mky, Ms, Rb, Rt WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

ATP2A2 / SERCA2 antibody

Western blot analysis of SERCA2 expression in THP1 (A); NIH3T3 (B); PC12 (C) whole cell lysates. Rabbit Aff - Purified Hu, Mky, Ms, Rb, Rt WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

ATP2A2 / SERCA2 antibody

Western Blot and Immunoprecipitation: SERCA2 Antibody [NB100-237] - Detection of Pig, Rat and Rabbit SERCA2 on HeLa whole cell lysate using NB100-237. Rabbit Aff - Purified Hu, Por, Rb, Rt IP, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

ATP2A2 / SERCA2 antibody

ATP2A2 / SERCA2 Mouse IgG1 IID8 Ascites Bov, Can, Hu, Por, Rb, Rt C, E, ICC/IF, IP, NEUT, P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

ATP2A2 / SERCA2 antibody

ATP2A2 / SERCA2 Mouse IgG2a 2A7-A1 Ascites Can, GP, Hu, Ms, Por, Rb, Rt C, ICC/IF, IP, P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

ATP2A2 / SERCA2 antibody

Western Blot: SERCA2 ATPase Antibody [NBP1-59203] - This Anti-ATP2A2 antibody was used in Western Blot of MCF7 tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Can, Dros, Hu, Ms, Por, Rt WB
50 µg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ATP2A2 / SERCA2

ATP2A2 / SERCA2 Human Purified
2 µg / €300.00
  Abnova Taiwan Corp.

13 Item(s)

per page
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