ATP2B4 / PMCA4 antibody

Principal name

ATP2B4 / PMCA4 antibody

Alternative names for ATP2B4 / PMCA4 antibody

ATP2B2, MXRA1, Calcium pump 4 ATPase, Plasma membrane calcium-transporting ATPase 4, Plasma membrane calcium ATPase isoform 4, Plasma membrane calcium pump isoform 4, Matrix-remodeling-associated protein 1

SwissProt ID

P23634 (Human), Q64542 (Rat)

Gene ID

493 (ATP2B4)

Available reactivities

Hu (Human), Por (Porcine), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Rb (Rabbit), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig)

Available hosts

Mouse, Rabbit

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Flow Cytometry (F), Frozen Sections (C), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunoprecipitation (IP)

Background of ATP2B4 / PMCA4 antibody

ATP2B4 belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. ATP2B4 is the plasma membrane calcium ATPase isoform 4.The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 4. Alternatively spliced transcript variants encoding different isoforms have been identified.

General readings

This product has been used in:Filoteo AG et al. Plasma membrane Ca2+ pump in rat brain. Patterns of alternative splices seen by isoform-specific antibodies. J Biol Chem 272:23741-7 (1997). PubMed PMID: 9295318
Caride AJ et al. Detection of isoform 4 of the plasma membrane calcium pump in human tissues by using isoform-specific monoclonal antibodies. Biochem J 316 (Pt 1):353-9 (1996). PubMed PMID: 8645230

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ATP2B4 / PMCA4 antibody

WB Suggested Anti-ATP2B4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: 721_B cell lysate.There is BioGPS gene expression data showing that ATP2B4 is expressed in 721_B Rabbit IgG Purified Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

ATP2B4 / PMCA4 antibody

ATP2B4 / PMCA4 Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

ATP2B4 / PMCA4 antibody

ATP2B4 / PMCA4 Mouse IgG1 2C7 Ascites Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

ATP2B4 / PMCA4 antibody

ATP2B4 / PMCA4 Mouse IgG1 2G8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ATP2B4 / PMCA4 antibody

Human 721_B; WB Suggested Anti-ATP2B4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: 721_B cell lysate. There is BioGPS gene expression data showing that ATP2B4 is expressed in 721_B.; ATP2B4 antibody - middle region (ARP44394_P050) in Human 721_B cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

ATP2B4 / PMCA4 antibody

Figure 1 illustrates immunolocalization of PMCA4 and CaSRO in human corneal epithelium. (A) Immunolocalization of PMCA4 (green) using NB300-569 on cell membranes of all cells in each layer of hCE. (B) Immunolocalization of CaSRO (red)on cell membranes of hCE. (C) Merged image.    Figure 2 illustrates immunolocalization of PMCA4 and CaSRO in bovine corneal epithelium (bCE). (A) Immunolocalization of PMCA4 (green) on cell membranes of all cells in each layer of bCE. (B) Immunolocalization of CaSRO (red) on cell membranes of bCE mostly in wing- and squamous cell layers. (C) Merged image. Mouse IgG1 JA9 Ig Fraction Hu, Por, Rt C, E, ICC/IF, P, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

ATP2B4 / PMCA4 (N-term) antibody

ATP2B4 / PMCA4 Rabbit IgG Serum Hu, Ms, Rt C, IP, P, WB
0.2 ml / €420.00
  Swant

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ATP2B4 / PMCA4 (transcript variant 2)

ATP2B4 / PMCA4 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

ATP2B4 / PMCA4

ATP2B4 / PMCA4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

ATP2B4 / PMCA4

ATP2B4 / PMCA4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

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