ATPase inhibitor ATPIF1 antibody

Principal name

ATPase inhibitor ATPIF1 antibody

Alternative names for ATPase inhibitor ATPIF1 antibody

ATPI, F1 F0 ATP synthase subunit IF1, Complex V subunit IF1, mitochondrial ATPase inhibitor

SwissProt ID

O35143 (Mouse), P01096 (Bovin), P37209 (Caeel), Q03344 (Rat), Q29307 (Pig), Q9UII2 (Human)

Gene ID

93974 (ATPIF1)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Por (Porcine), Rt (Rat), Ze (Zebrafish), African clawed frog, Xen (Xenopus)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P)

Background of ATPase inhibitor ATPIF1 antibody

Mitochondrial ATP synthases (ATPases) transduce the energy contained in membrane electrochemical proton gradients into the energy required for synthesis of high-energy phosphate bonds. ATPases contain two linked complexes: F1, the hydrophilic catalytic core; and F0, the membrane-embedded protein channel. F1 consists of three Alpha chains and three Beta chains, which are weakly homologous, as well as one Gamma chain, one Delta chain and one Gamma chain. F0 consists of three subunits: a, b and c. A mitochondrial F1-ATPase inhibitor protein, ATPIF1 (ATPase inhibitory factor 1), also known as IP, IF1, ATPI or ATPIP (ATPase inhibitor protein), binds to the C-terminal region of a Beta subunit of the F1-ATPase at low pH values and, via interference of the Beta and Gamma subunit interaction, ATPIF1 regulates the activity of the F1F0-ATPase. This reversible ATPIF1 binding to F1F0-ATPase also occurs on the surface of endothelial cells.

General readings

Sanchez Cenizo L., et al. (2010) J Biol Chem. 285: 25308-13.Cortes Hernandez P., et al. (2005) Biochem Biophys Res Commun. 330: 844-849.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ATPase inhibitor ATPIF1 antibody

Immunofluorescence of monoclonal antibody to ATPIF1 on MCF-7 cell . [antibody concentration 10 ug/ml] Mouse IgG2b 3E2 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ATPase inhibitor ATPIF1 antibody

Predicted band size: 12 kDa . Positive control: Hela, Jurkat, MCF7 and A431 cell lysate. Recommended dilution: 1/1000-5000. (Gel: 16%+12%+10%SDS-PAGE Lane1: Hela cell lysate Lane2: Jurka cell lysate Lane3: MCF7 cell lysate Lane4: A431 cell lysate Lysates: 40 ug per lane Primary antibody: 1/1200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ATPase inhibitor ATPIF1 antibody

Predicted band size: 12 kDa . Positive control: Hela, Jurkat, MCF7 and A431 cell lysate. Recommended dilution: 1/1000-5000. (Gel: 16%+12%+10%SDS-PAGE Lane1: Hela cell lysate Lane2: Jurkat cell lysate Lane3: MCF7 cell lysate Lane4: A431 cell lysate Lysates: 40 ug per lane Primary antibody: 1/1350 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ATPase inhibitor ATPIF1 antibody

Immunofluorescence of purified MaxPab antibody to ATPIF1 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

ATPase inhibitor ATPIF1 antibody

Human 293T; WB Suggested Anti-ATPIF1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: 293T cell lysate. ATPIF1 is supported by BioGPS gene expression data to be expressed in HEK293T.; ATPIF1 antibody - N-terminal region (ARP48451_P050) in Human 293T cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Hu, Ms, Por, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

ATPase inhibitor ATPIF1 antibody

ATPase inhibitor ATPIF1 Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rt, Xen, Ze WB
50 µg / €440.00
  Novus Biologicals Inc.

ATPase inhibitor ATPIF1 antibody

Immunohistochemistry-Paraffin: ATPase Inhibitory Factor 1 Antibody [NBP1-90069] - Immunohistochemical staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ATPase inhibitor ATPIF1 (transcript variant 1)

ATPase inhibitor ATPIF1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

ATPase inhibitor ATPIF1 (transcript variant 3)

ATPase inhibitor ATPIF1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

ATPase inhibitor ATPIF1 (26-106, His-tag)

ATPase inhibitor ATPIF1 Human Purified > 95 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

ATPase inhibitor ATPIF1 (26-106, His-tag)

ATPase inhibitor ATPIF1 Human Purified > 95 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

ATPase inhibitor ATPIF1

ATPase inhibitor ATPIF1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

ATPase inhibitor ATPIF1

ATPase inhibitor ATPIF1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

ATPase inhibitor ATPIF1 Lysate (Non-Denatured)

ATPase inhibitor ATPIF1 Lysate (Non-Denatured)  
0.1 mg / €270.00
  Abnova Taiwan Corp.

ATPIF1 293T Cell Transient Overexpression Lysate(Denatured)

ATPIF1 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

ATPIF1 overexpression lysate

ATPIF1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

ATPIF1 overexpression lysate

ATPIF1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

17 Item(s)

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