ATPase WRNIP1 antibody

Principal name

ATPase WRNIP1 antibody

Alternative names for ATPase WRNIP1 antibody

WRNIP1, WHIP, Werner helicase-interacting protein 1

SwissProt ID

Q8CG07 (Rat), Q91XU0 (Mouse), Q96S55 (Human)

Gene ID

56897 (WRNIP1), 282835 (Wrnip1)

Available reactivities

Bov (Bovine), Dros (Drosophila), Hu (Human), Ms (Mouse), Rt (Rat), Ze (Zebrafish), Mky (Monkey), Can (Canine), Por (Porcine), Ye (Yeast)

Available hosts

Rabbit, Goat

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P)

Background of ATPase WRNIP1 antibody

Werner\'s syndrome is a rare autosomal recessive disorder characterized by premature aging. The protein encoded by this gene interacts with the N-terminal portion of Werner protein containing the exonuclease domain. This protein shows homology to replication factor C family proteins, and is conserved from E. coli to human. Studies in yeast suggest that this gene may influence the aging process. Two transcript variants encoding different isoforms have been isolated for this gene.Werner\'s syndrome is a rare autosomal recessive disorder characterized by premature aging. The protein encoded by this gene interacts with the N-terminal portion of Werner protein containing the exonuclease domain. This protein shows homology to replication factor C family proteins, and is conserved from E. coli to human. Studies in yeast suggest that this gene may influence the aging process. Two transcript variants encoding different isoforms have been isolated for this gene.

General readings

Bish,R.A. (2007) J. Biol. Chem. 282 (32), 23184-23193

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ATPase WRNIP1 (C-term) antibody

  Goat Anti-WHIP / WRNIP1 Antibody  
(1ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ATPase WRNIP1 antibody

  Rabbit polyclonal anti-WHIP antibody  
WB analysis using Anti-Human WHIP antibody to detect Human WHIP present in a HEK293 whole cell lysate.  ~30µg of lysate was loaded per lane for 4-20% gradient SDS-PAGE. See Figure 2 for the results of peptide competition experiments. The blot was incubated with a 1:200 dilution of the antibody at RT for 2 h followed by detection using IRDye® 800 labeled Goat-a-Rabbit IgG [H&L] MX10  diluted 1:5,000 for 45 min. Rabbit IgG Aff - Purified Hu, Mky, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ATPase WRNIP1 antibody

  Rabbit Polyclonal Anti-WRNIP1 Antibody  
WB Suggested Anti-WRNIP1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Hu, Ms, Por, Rt, Ye, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

ATPase WRNIP1 antibody

Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Kidney. Rabbit Aff - Purified Hu E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

ATPase WRNIP1 antibody

ATPase WRNIP1 Goat Hu, Ms, Rt E
0.1 mg / €400.00
  Abnova Taiwan Corp.

ATPase WRNIP1 antibody

Fig. 1
Western blot analysis is shown using WRNIP1 polyclonal antibody ( Cat # PAB10242 ) to detect human WRNIP1 present in a HEK293 whole cell lysate.
Approximately 30 µg of lysate was loaded per lane for 4-20% gradient SDS-PAGE.
Comparison to a molecular weight marker ( not shown ) indicates a primary band of ~96.0 kDa is detected.
The identity of the minor band migrating at a slightly higher molecular weight is unknown, but may represent an alternate isoform of WRNIP1 or post translational modification of the WRNIP1 protein.
See Figure 2 for the results of peptide competition experiments.
The blot was incubated with a 1 : 200 dilution of the antibody at room temperature for 2 h followed by detection using IRDye® 800 labeled Goat-a-Rabbit IgG [H&L] MX10 diluted 1 : 5,000 for 45 min. Hu, Mky, Ms, Rt
0.1 mg / €560.00
  Abnova Taiwan Corp.

ATPase WRNIP1 antibody

Human; Sample Type: HeLa cell lysateDilution: 1:1000; WRNIP1 antibody - N-terminal region (ARP36470_P050) in Human cells using Western Blot Rabbit Aff - Purified Bov, Dros, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

CRISPR

Catalog No. Species Donor Vector  

WRNIP1 - human gene knockout kit via CRISPR

WRNIP1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

WRNIP1 - human gene knockout kit via CRISPR

WRNIP1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

WRNIP1 - human gene knockout kit via CRISPR

WRNIP1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

WRNIP1 - human gene knockout kit via CRISPR

WRNIP1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ATPase WRNIP1 (transcript variant 2)

  Recombinant protein of human Werner helicase interacting protein 1 (WRNIP1), transcript variant 2  
ATPase WRNIP1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Lysates

Catalog No.    

WRNIP1 overexpression lysate

WRNIP1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

WRNIP1 overexpression lysate

WRNIP1 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, WRNIP1 (mGFP-tagged)-Human Werner helicase interacting protein 1 (WRNIP1), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, WRNIP1 (mGFP-tagged)-Human Werner helicase interacting protein 1 (WRNIP1), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,340.00
  OriGene Technologies, Inc.

Lenti ORF particles, WRNIP1 (mGFP-tagged) - Human Werner helicase interacting protein 1 (WRNIP1), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, WRNIP1 (mGFP-tagged) - Human Werner helicase interacting protein 1 (WRNIP1), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, WRNIP1 (Myc-DDK-tagged)-Human Werner helicase interacting protein 1 (WRNIP1), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, WRNIP1 (Myc-DDK-tagged)-Human Werner helicase interacting protein 1 (WRNIP1), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,200.00
  OriGene Technologies, Inc.

Lenti ORF particles, WRNIP1 (Myc-DDK tagged) - Human Werner helicase interacting protein 1 (WRNIP1), transcript variant 2 , 200 uL, >10^7 TU/mL

Lenti ORF particles, WRNIP1 (Myc-DDK tagged) - Human Werner helicase interacting protein 1 (WRNIP1), transcript variant 2 , 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Wrnip1 (GFP-tagged ORF) - Rat Werner helicase interacting protein 1 (Wrnip1), 200 uL, >10^7 TU/mL

Lenti ORF particles, Wrnip1 (GFP-tagged ORF) - Rat Werner helicase interacting protein 1 (Wrnip1), 200 uL, >10^7 TU/mL  
200 µl / €1,330.00
  OriGene Technologies, Inc.

Lenti ORF particles, Wrnip1 (Myc-DDK-tagged ORF) - Rat Werner helicase interacting protein 1 (Wrnip1), 200 uL, >10^7 TU/mL

Lenti ORF particles, Wrnip1 (Myc-DDK-tagged ORF) - Rat Werner helicase interacting protein 1 (Wrnip1), 200 uL, >10^7 TU/mL  
200 µl / €1,193.00
  OriGene Technologies, Inc.

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