AUP1 antibody

Principal name

AUP1 antibody

Alternative names for AUP1 antibody

Ancient ubiquitous protein 1

SwissProt ID

A1L134 (Rat), P70295 (Mouse), Q9Y679 (Human)

Gene ID

550 (AUP1)

Available reactivities

Bov (Bovine), Hu (Human), Rt (Rat), Sh (Sheep), Ms (Mouse)

Available hosts

Sheep, Rabbit

Available applications

Frozen Sections (C), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P)

Background of AUP1 antibody

This gene encodes a protein that contains a domain with homology to the ancient conserved region of the archain 1 gene and a domain that may be involved in binding ubiquitin-conjugating enzymes. The protein encoded by this gene has been shown to bind to the conserved membrane-proximal sequence of the cytoplasmic tail of integrin alpha(IIb) subunits. These subunits play a crucial role in the integrin alpha(IIb)beta(3) inside-out signalling in platelets and megakaryocytes that leads to platelet aggregation and thrombus formation. This gene overlaps the gene for mitochondrial serine protease 25.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

AUP1 antibody

Predicted band size: 53 kDa . Positive control: Hela and 293T cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lane1: Hela cell lysate Lane2: 293T cell lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

AUP1 antibody

Predicted band size: 53 kDa . Positive control: Hela and 293T cell, mouse liver tissue and NIH/3T3 cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: Hela cell lysate Lane2: 293Tcell lysate Lane3: Mouse liver tissue lysate Lane4: NIH/3T3 cell lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

AUP1 antibody

Host: Rabbit; Target Name: Aup1; Sample Tissue: Mouse Heart lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Hu, Ms WB
50 µg / €325.00
  OriGene Technologies, Inc.

AUP1 (C-term) antibody

AUP1 Antibody (C-term) (Cat. #AP50315PU-N) western blot analysis in Hela cell line lysates (35µg/lane).This demonstrates the AUP1 antibody detected the AUP1 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

AUP1 antibody

AUP1 Sheep Bov, Hu, Rt, Sh C, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

AUP1 antibody

Western Blot: Ancient ubiquitous protein 1 Antibody [NBP1-49916] - Detection of Human AUP1 by Western Blot and Immunoprecipitation.  Samples:  Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells.  Antibodies: Affinity purified rabbit anti-AUP1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate.  AUP1 was also immunoprecipitated by rabbit anti-AUP1 antibody BL10556, which recognizes an upstream epitope.  For blotting immunoprecipitated AUP1 wase used.  Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B). Rabbit Aff - Purified Hu IP, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

AUP1 antibody

Immunohistochemistry-Paraffin: Ancient ubiquitous protein 1 Antibody [NBP1-85785] - Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

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