Aurora kinase A antibody

Principal name

Aurora kinase A antibody

Alternative names for Aurora kinase A antibody

AURKA, AIK1, ARK1, AURA, BTAK, STK15, STK6, Aurora/IPL1-related kinase 1, AURORA2, Serine/threonine-protein kinase 6, Serine/threonine kinase 15, Breast tumor-amplified kinase

SwissProt ID

A5GFW1 (Pig), O14965 (Human), P59241 (Rat), P97477 (Mouse), Q2TA06 (Bovin)

Gene ID

6790 (AURKA), 20878 (Aurka)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey), Prim (Primate), Bov (Bovine), Can (Canine), Frog, Por (Porcine), Mam (Mammalia), Sh (Sheep), Eq (Equine), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Goat

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Frozen Sections (C), Flow Cytometry (F), Immunofluorescence (IF), Immunohistochemistry (IHC)

Background of Aurora kinase A antibody

Aurora A (also known as Aurora 2) is a serine/threonine kinase with a molecular weight of approximately 46 kD. This kinase is highly expressed in the thymus and some tumors and is also expressed in other tissues including the lung, testis, colon, placenta, and fetal liver. Aurora A localizes in the midzone or central spindle in late anaphase and is concentrated in the midbody in telophase and during cytokinesis. This kinase is believed to act in cell cycle regulation during anaphase and/or telophase at centrosome/spindle pole during chromosome segregation. Aurora A has been shown to regulate cleavage of polar spindle microtubules at the onset of cytokinesis during mitosis. Defects in Aurora A cause numerous centrosome aberrations including aneuploidy (genetic variant with amino acid substitution F31I). Aurora A expression is cell cycle regulated, low in G1/S, and accumulating in G2/M. Expression is upregulated in cancer cells during M phase. Phosphorylation by PKA has been shown to regulate function. Aurora A phosphorylation has been reported on Thr 288. This kinase associates with the centrosome and mitotic spindles, NM23-H1, protein phosphatase type I, and co-localizes with ?;-tubulin. The Phe 31 variant has been shown to interact with the E2 ubiquitin-conjugating enzyme, UBE2N. The Poly6187 antibody has been shown to react with phosphorylated human Aurora A. This antibody has weak avidity for Aurora B in immunofluorescence. To minimize;Aurora B cross-reactivity, it is recommended that the reagent be titrated and more stringent wash conditions be employed.

General readings

1. Bischoff, J. R., Plowman, G. D. The Aurora/lpl 1p kinase family: regulators of chromosome
segregation and cytokinesis. Trends Cell Biol. 9: 454-459, 1999.
2. Hutterer, A., Berdnik, D., Wirtz-Peitz, F., Zigman, M., Schleiffer, A., Knoblich, J. A. Mitotic activation
of the kinase Aurora-A requires its binding partner Bora. Dev. Cell 11: 147-157, 2006.
3. Seki, A., Coppinger, J. A., Jang, C.-Y., Yates, J. R., III, Fang, G. Bora and the kinase Aurora A
cooperatively activate the kinase Plk1 and control mitotic entry. Science 320: 1655-1658, 2008.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Aurora-A/B (C-term) (incl. pos. control) antibody

Detection of endogenous Aurora-A/B: Whole cell extracts of vanadate treated tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The Immunoblot was probed with monoclonal Aurora-A/B antibody AM20208PU-N (5F11) (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).
Lane 1: A431 
Lane 2: SW480 
Lane 3: SW620 
Lane 4: HT29
Lane 5: MCF-7 
Lane 6: MDA-MB231 
Lane 7: T47D Mouse IgG1 5F11 Purified Hu F, ICC/IF, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

Aurora kinase A antibody

Simple Western: Aurora A Antibody [TA336807] - Simple Western lane view shows a specific band detected for Aurora A in HeLa lysate.  This experiment was performed under reducing conditions using the Wes or Sally Sue separation system 12-230kDa (or 66-440k Rabbit Purified Hu, Ms C, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Aurora kinase A antibody

Immunofluorescence analysis of Hela cells using AURKA antibody Cat.-No AM06572SU-N (green). Blue: DRAQ5 fluorescent DNA dye. Mouse IgG1 1F8 Ascites Hu, Mky, Rt E, ICC/IF, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

Aurora kinase A (C-term) antibody

TA302646 (0.1µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Aurora kinase A antibody

Western blot analysis of Aurora Kinase expression in serum treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Aurora kinase A antibody

Western blot analysis of Aurora Kinase phosphorylation expression in serum treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Aurora kinase A antibody

Western blot analysis of extracts of various cell lines, using AURKA antibody. Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Aurora kinase A antibody

Hela cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-Aurora A antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. Rabbit IgG Poly6033 Purified Hu, Ms ICC/IF, WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

Aurora kinase A antibody

Panel A. Extracts from untreated Hela cells (Lane 1) or overnight nocodazole-treated Hela cells (Lane 2) were immunoprecipitated with a pan-Aurora A monoclonal antibody (clone 35C1), resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit polyclonal antibody against Thr288 phosphorylated Aurora A (Poly6187). Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. Rabbit IgG Poly6187 Purified Hu ICC/IF, P, WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

Aurora kinase A antibody

Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using Phospho-AURKA-T288 antibody. Rabbit IgG Purified Hu, Ms
0.1 mg / €325.00
  OriGene Technologies, Inc.

Aurora kinase A antibody

Host: Rabbit; Target Name: AURKA; Sample Tissue: Thyroid Tumor lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Aurora kinase A antibody

Western Blot: Aurora A Antibody (1F8) [TA336917] - Western blot analysis using Aurora A mouse mAb against HEK293 (1), Sw620 (2), MCF-7 (3), Jurkat (4), Hela (5), HepG2 (6), Cos7 (7) and PC-12 (8) cell lysate. Mouse IgG1 1F8 Purified Hu, Prim, Rt E, F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Aurora kinase A antibody

Western blot detection of Aurora Kinase A in SW480 and COS7 cell lysates and using Aurora Kinase A mouse mAb (1:500 diluted).Predicted band size: 46KDa.Observed band size:46KDa. Mouse IgG1 2E3-C10-C11-G6 Purified Hu, Mky ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Aurora kinase A antibody

Aurora kinase A Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Aurora kinase A antibody

Panel A. Extracts from untreated Hela cells (Lane 1) or overnight nocodazole-treated Hela cells (Lane 2) were immunoprecipitated with a pan-Aurora A monoclonal antibody (clone 35C1), resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit polyclonal antibody against Thr288 phosphorylated Aurora A (Poly6187). Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. Panel B. The blot shown in Panel A was stripped and re-probed with pan-Aurora specific polyclonal antibody (Poly6033) to verify equal loading of Aurora A in the extracts. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. Rabbit Poly6187 Purified Hu ICC/IF, P, WB
50 µl / €280.00
  OriGene Technologies, Inc.

Aurora kinase A antibody

Western blot analysis of HeLa (lane 1), 293 (lane2), NIH3T3 (lane 3), and mouse thymus (lane 4) cells using anti-Aurora A antibody (Clone Poly6033). Beta-actin (Poly6221) was used as loading control. Rabbit Poly6033 Purified Hu ICC/IF, WB
50 µl / €248.00
  OriGene Technologies, Inc.

Aurora kinase A antibody

Immunohistochemical analysis of paraffin-embedded ovarian cancer (left) and lung cancer (right) using AURKA antibody Cat.-No AM06573SU-N with DAB staining. Mouse IgG1 4G10 Ascites Hu, Mky, Rt E, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

Aurora kinase A (N-term) (incl. pos. control) antibody

Detection of endogenous Aurora-A: Whole cell extracts of vanadate treated tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The Immunoblot was probed with monoclonal antibody AM20207PU-N (7F12) (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).
Lane 1: A431 
Lane 2: SW480 
Lane 3: SW620 
Lane 4: HT29
Lane 5: MCF-7 
Lane 6: MDA-MB231 
Lane 7: T47D Mouse IgG1 7F12 Purified Can, Hu, Ms, Rt WB
0.1 mg / €430.00
  Acris Antibodies GmbH

Aurora kinase A (full length) antibody

Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgM 7A3 Aff - Purified Hu ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Aurora kinase A antibody

Immunohistochemistry (IHC) analyuzes of AURKA antibody (Cat.-No.: AP06626PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

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