Aurora kinase B antibody

Principal name

Aurora kinase B antibody

Alternative names for Aurora kinase B antibody

Aurora-B, Aurora/IPL1-related kinase 2, STK-1, AURKB, AIK2, AIM1, ARK2, STK12, Aurora- and Ipl1-like midbody-associated protein 1, Serine/threonine-protein kinase 12

SwissProt ID

O55099 (Rat), O70126 (Mouse), Q7YRC6 (Bovin), Q96GD4 (Human), Q9N0X0 (Pig)

Gene ID

9212 (AURKB), 8379 (MAD1L1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Mky (Monkey), Por (Porcine), Chimp (Chimpanzee), Chk (Chicken), Eq (Equine)

Available hosts

Rabbit, Mouse, Sheep, Goat

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E)

Background of Aurora kinase B antibody

AURKB(aurora kinase B) also called Aik2, AIM-1, ARK2, AurB, STK12, IPL1, PPP1R48 or STK5, localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers. Cell cycle and Northern blot analyses showed that STK12 is expressed in the S phase and persistently thereafter. Western blot analysis indicated that STK12 is localized in the midbodies during anaphase. Northern blot analysis detected strong expression of a 1.5-kb STK12 transcript in thymus, with weaker expression in small intestine, testis, colon, spleen, and brain. The AURKB gene is mapped on 17p13.1. Examination of the role of both kinases in the phosphorylation of CENPA revealed that the reaction is mediated sequentially by AURKA and AURKB in early mitosis. EB1 overexpression enhanced AURKB kinase activity, and knockdown of EB1 with small interfering RNA impaired AURKB activity. EB1 protected AURKB from dephosphorylation/inactivation by protein phosphatase-2A by blocking binding of PP2A to AURKB. 

General readings

Shindo, M., et al. 1998. Biochem. Biophys. Res. Commun. 244: 285 Bischoff, J.R., et al. 1999. Trends Cell Biol. 9:454 Andrews, P. D., et al. 2003. Opin. Cell Biol. 15: 672

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Aurora kinase B pThr232 antibody

Untreated Hela cells (Panel A) stained with purified rabbit polyclonal antibody against Aurora B Phospho (Thr232), followed by Cy3 conjugated Donkey anti-rabbit IgG and DAPI. Rabbit IgG Aff - Purified Hu ICC/IF
25 µl / €230.00
  Acris Antibodies GmbH

Aurora kinase B antibody

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-AURKB rabbit polyclonal antibody. (TA590640) Rabbit IgG Purified Can, Hu, Mky, Ms E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Aurora kinase B pThr232 antibody

Western Blot shows detection of Aurora B protein at 39 kDa (predicted band size). All lanes : Aurora B (phospho T232) antibody diluted 1:500. Lane 1 : Extract from COS7 cells treated with Nocodazole (1ug/ml, 16 hrs). Lane 2 : Extract from COS7 cells treated with Nocodazole (1ug/ml, 16 hrs) and with the phosphopeptide immunogen. Rabbit IgG Aff - Purified Bov, Can, Chimp, Hu, Ms, Por, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Aurora kinase B antibody

Western blot analysis of AurB expression in Nocodazole treated COS7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Aurora kinase B antibody

TA348927 (1 ug/ml) staining of Thymus (A), Lymph Node (B), Tonsil (C), Spleen (D) and Testis (E) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Aurora kinase B antibody

Western blot analysis using AURKB antibody Cat.-No  AM06156SU-N against truncated AURKB recombinant protein (Lane 1) and SKN-SH cell lysate (Lane 2). Mouse IgG1 13E8D3 Ascites Hu WB
0.1 ml / €440.00
  Acris Antibodies GmbH

Aurora kinase B antibody

Immunofluorescence of human HeLa cells stained with Hoechst 3342 (Blue) for chromatin staining and monoclonal anti-human Aurora kinase B antibody Cat.-No. AM09372PU (1:2000) with Texas Red (Red). Mouse IgG2b AT2B1 Purified Hu E, F, ICC/IF, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

Aurora kinase B antibody

Immunofluorescence of human HeLa cells stained with Hoechst 3342 (Blue) for chromatin staining and monoclonal anti-human Aurora kinase B antibody Cat.-No. AM09372PU (1:2000) with Texas Red (Red). Mouse IgG2b AT2B1 Purified Hu E, F, ICC/IF, WB
50 µl / €230.00
  Acris Antibodies GmbH

Aurora kinase B antibody

Aurora kinase B Rabbit IgG Purified Bov, Can, Hu, Mky, Ms, Por, Rt E, WB
50 µg / €380.00
  Acris Antibodies GmbH

Aurora kinase B antibody

Western blot (WB) analysis of AURKB antibody (Cat.-No.: AP06597PU-N) at 1/500 dilution Lane1:HEK293T whole cell lysate Lane2:Mouse liver tissue lysate Lane3:PC12 whole cell lysate Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Aurora kinase B antibody

Immunohistochemistry analyzes of AurB antibody (Cat.-No.: AP06609PU-N) in paraffin-embedded Human liver carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Aurora kinase B (227-238) antibody

Spleen: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Aurora kinase B (223-234) pThr232 antibody

Tonsil: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Aurora kinase B (1-344) antibody

Human Tonsil (formalin-fixed, paraffin-embedded) stained with AURKB antibody AP22823PU-N at 10 µg/ml followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen. Sheep Purified Hu P, WB
50 µl / €470.00
  Acris Antibodies GmbH

Aurora kinase B antibody

Human Tonsil, Lymphocytes: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Aurora kinase B (N-term) antibody

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Aurora kinase B pThr232 antibody

Western blot analysis of extracts from COS7 cells treated with Nocodazole using Phospho-AurB (Thr232) antibody AP55931PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 ml / €340.00
  Acris Antibodies GmbH

Aurora kinase B pThr232 antibody

Western blot analysis of extracts from COS7 cells treated with Nocodazole using Phospho-AurB (Thr232) antibody AP55931PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µl / €270.00
  Acris Antibodies GmbH

Aurora kinase B antibody

Figure shows a model of Aurora B, INCENP, and
Survivin interactions and regulation. Survivin binds the N
terminus of INCENP, whereas the N terminus of Aurora B
binds the C terminus of INCENP. See Bolton et al. (2002). Rabbit Aff - Purified Hu E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

Aurora kinase B antibody

AURKB monoclonal antibody (M01), clone 6A6 Western Blot analysis of AURKB expression in HepG2 ( Cat # L019V1 ). Mouse IgG1 6A6 Ascites Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

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