Axin-1 antibody

Principal name

Axin-1 antibody

Alternative names for Axin-1 antibody

AXIN1, AXIN, Axis inhibition protein 1

SwissProt ID

O15169 (Human), O35625 (Mouse), O42400 (Chick), O70239 (Rat)

Gene ID

8312 (AXIN1), 12005 (Axin1), 79257

Available reactivities

Hu (Human), Bt (Bat), Bov (Bovine), Can (Canine), Chk (Chicken), Eq (Equine), Fi (Fish), Hst (Hamster), Mky (Monkey), Ms (Mouse), Op (Opossum), Prim (Primate), Rt (Rat), Xen (Xenopus), Tr (Turkey), Bear

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of Axin-1 antibody

Axin-1 (Axis inhibition protein 1) and Axin-2 are multidomain scaffold proteins that negatively regulate Wnt signaling. Axin-1 interacts with APC, GSK3-beta, Dvl and beta-Catenin and promotes the GSK3-beta-mediated phosphorylation and subsequent degradation of beta-Catenin (1,2). Upon stimulation of cells with Wnt, Axin1 is recruited to the membrane by phosphorylated LRP5/6, a process that is believed to be crucial for activation of Wnt signaling (3,4). In addition to its role in the Wnt signaling pathway, Axin1 forms a complex with MEKK1 and activates c-Jun amino-terminal kinase (JNK/SAPK) (5). Axin2 (also known as Conductin or Axil) can functionally substitute Axin1 in mice (6). Axin-2 itself is a direct target of the Wnt signaling pathway and therefore serves to control the duration and/or intensity of Wnt signaling through a negative feedback loop (7-9).

General readings

Zeng L, Fagotto F, Zhang T, Hsu W, Vasicek TJ, Perry WL 3rd, Lee JJ, Tilghman SM, Gumbiner BM, Costantini F.
The mouse Fused locus encodes Axin, an inhibitor of the Wnt signaling pathway that regulates embryonic axis formation.
Cell. 1997 Jul 11;90(1):181-92.
PMID: 9230313

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Axin-1 (C-term) antibody

TA305807 (3.8ug/ml) staining of paraffin embedded Human Adrenal Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat Aff - Purified Bov, Can, Hu, Ms, Rt P
0.1 mg / €325.00
  OriGene Technologies, Inc.

Axin-1 antibody

Sample(30 ug of whole cell lysate). A:A431. B:H1299. 7.5% SDS PAGE. TA308745 diluted at 1:1500 Rabbit IgG Aff - Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Axin-1 (C-term) antibody

Western blot analysis of AXIN1 in SK-N-SH cell lysate with AXIN1 antibody at 1 ug/mL. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Axin-1 antibody

Immunofluorescence of stained human HeLa cells was done with Hoechst 33342 (Blue) for chromatin staining and Texas Red (Red) for monoclonal anti-human Axin1a antibody (1:500). Mouse IgG2b AT1A4 Purified Hu E, ICC/IF, IP, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

Axin-1 antibody

Immunofluorescence of stained human HeLa cells was done with Hoechst 33342 (Blue) for chromatin staining and Texas Red (Red) for monoclonal anti-human Axin1a antibody (1:500). Mouse IgG2b AT1A4 Purified Hu E, ICC/IF, IP, WB
50 µl / €230.00
  Acris Antibodies GmbH

Axin-1 (850-862) antibody

Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat IgG Aff - Purified Bear, Bov, Bt, Can, Chk, Elephant, Eq, Fi, Hst, Hu, Mky, Ms, Op, Prim, Rt, Tr, Xen E, P
50 µg / €470.00
  Acris Antibodies GmbH

Axin-1 control peptide

Axin-1
50 µg / €160.00
  Acris Antibodies GmbH

Axin-1 (C-term) antibody

AXIN1 Antibody (C-term) (Cat. #AP50319PU-N)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of AXIN1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Axin-1 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between GSK3B and AXIN1 HeLa cells were stained with anti-GSK3B rabbit purified polyclonal 1:1200 and anti-AXIN1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG2a 2B11 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Axin-1 antibody

Axin-1 Goat Hu E
0.1 mg / €400.00
  Abnova Taiwan Corp.

Axin-1 antibody

Western blot analysis of Axin-1 expression in HEK293T (A); NIH3T3 (B); PC12 (C) whole cell lysates. Rabbit Aff - Purified Hu, Mky, Ms, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

Axin-1 antibody

Western blot analysis of Axin-1 expression in HEK293T (A); NIH3T3 (B); PC12 (C) whole cell lysates. Rabbit Aff - Purified Hu, Mky, Ms, Rt WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

Axin-1 antibody

Western blot analysis of Axin-1 expression in HEK293T (A); NIH3T3 (B); PC12 (C) whole cell lysates. Rabbit Aff - Purified Hu, Mky, Ms, Rt WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

CRISPR

Catalog No. Species Donor Vector  

AXIN1 - human gene knockout kit via CRISPR

AXIN1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

AXIN1 - human gene knockout kit via CRISPR

AXIN1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

AXIN1 - human gene knockout kit via CRISPR

AXIN1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

AXIN1 - human gene knockout kit via CRISPR

AXIN1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Axin1 - mouse gene knockout kit via CRISPR

Axin1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Axin-1 (transcript variant 2)

Axin-1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Axin-1

Axin-1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

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