B-cell linker protein / BLNK antibody

Principal name

B-cell linker protein / BLNK antibody

Alternative names for B-cell linker protein / BLNK antibody

BASH, SLP65, SLP-65, Cytoplasmic adapter protein, B-cell adapter containing a SH2 domain protein, B-cell adapter containing a Src homology 2 domain protein, Src homology 2 domain-containing leukocyte protein of 65 kDa

SwissProt ID

Q4KM52 (Rat), Q8WV28 (Human), Q9QUN3 (Mouse), Q9YGC1 (Chick)

Gene ID

17060 (Blnk), 29760 (BLNK)

Available reactivities

Ms (Mouse), Hu (Human), Rt (Rat), Bov (Bovine), Can (Canine), Por (Porcine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Goat, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunoprecipitation (IP), Immunoelectron Microscopy (EM), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Flow Cytometry (F)

Background of B-cell linker protein / BLNK antibody

This gene encodes a cytoplasmic linker or adaptor protein that plays a critical role in B cell development. This protein bridges B cell receptor-associated kinase activation with downstream signaling pathways, thereby affecting various biological functions. The phosphorylation of five tyrosine residues is necessary for this protein to nucleate distinct signaling effectors following B cell receptor activation. Mutations in this gene cause hypoglobulinemia and absent B cells, a disease in which the pro- to pre-B-cell transition is developmentally blocked. Deficiency in this protein has also been shown in some cases of pre-B acute lymphoblastic leukemia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

1. Fu C, Turck CW, Kurosaki T, Chan AC: BLNK: a central linker protein in B cell activation. Immunity. 1998 Jul;9(1):93-103.
2. Jumaa H, Mitterer M, Reth M, Nielsen PJ: The absence of SLP65 and Btk blocks B cell development at the preB cell receptor-positive stage. Eur J Immunol. 2001 Jul;31(7):2164-9.
3. Kersseboom R, Middendorp S, Dingjan GM, Dahlenborg K, Reth M, Jumaa H, Hendriks RW: Bruton's tyrosine kinase cooperates with the B cell linker protein SLP-65 as a tumor
suppressor in Pre-B cells. J Exp Med. 2003 Jul 7;198(1):91-8.
5. Kersseboom R, Ta VB, Zijlstra AJ, Middendorp S, Jumaa H, van Loo PF, Hendriks RW: Bruton's tyrosine kinase and SLP-65 regulate pre-B cell differentiation and the induction of Ig light chain gene rearrangement. J Immunol. 2006 Apr 15;176(8):4543-52.
6. Koretzky GA, Abtahian F, Silverman MA: SLP76 and SLP65: complex regulation of signalling in lymphocytes and beyond. Nat Rev Immunol. 2006 Jan;6(1):67-78.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

B-cell linker protein / BLNK antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-BLNK mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808517)(1:150) Mouse IgG2b OTI4C9 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

B-cell linker protein / BLNK antibody

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using BLNK mouse mAb with DAB staining. Mouse IgG1 5G9 Ascites Hu, Ms E, F, ICC/IF, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

B-cell linker protein / BLNK (C-term) antibody

TA302649 staining (4µg/ml) of Daudi lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, WB
20 µg / €200.00
  Acris Antibodies GmbH

B-cell linker protein / BLNK (C-term) antibody

TA302649 staining (4µg/ml) of Daudi lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

B-cell linker protein / BLNK antibody

Sample (30 ug of whole cell lysate). A: Hela. 10% SDS PAGE. TA308826 diluted at 1:500. Rabbit IgG Aff - Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

B-cell linker protein / BLNK antibody

Western blot analysis of extracts from 293 cells, treated with etoposide (25uM, 24hours), using BLNK (Ab-96) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

B-cell linker protein / BLNK antibody

Western blot analysis of BLNK expression in etoposide treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

B-cell linker protein / BLNK antibody

WB Suggested Anti-BLNK Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: NCI-H226 cell lysate Rabbit IgG Purified Bov, Can, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

B-cell linker protein / BLNK antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BLNK (RC202488, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BLNK.(1:2000) Mouse IgG1 OTI2B2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

B-cell linker protein / BLNK antibody

B-cell linker protein / BLNK Rabbit IgG Purified Ms WB
0.1 mg / €340.00
  Acris Antibodies GmbH

B-cell linker protein / BLNK (Center) antibody

Western blot analysis of BLNK Antibody (Center) (Cat. #AP17153PU-N) in NCI-H460 cell line lysates (35ug/lane). BLNK (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

B-cell linker protein / BLNK pTyr96 antibody

Western blot analysis of extracts from COLO205 tissue using BLNK (Phospho-Tyr96) antibody AP55915PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms WB
0.1 ml / €340.00
  Acris Antibodies GmbH

B-cell linker protein / BLNK pTyr96 antibody

Western blot analysis of extracts from COLO205 tissue using BLNK (Phospho-Tyr96) antibody AP55915PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms WB
50 µl / €270.00
  Acris Antibodies GmbH

B-cell linker protein / BLNK antibody

Western Blotting analysis (reducing conditions) of whole cell lysate of Sp2/0 mouse myeloma cell line using polyclonal anti-mouse BLNK antibody. Rabbit Purified Ms WB
0.1 mg / €260.00
  Acris Antibodies GmbH

B-cell linker protein / BLNK antibody

Western Blot analysis of BLNK expression in transfected 293T cell line (H00029760-T03) by BLNK MaxPab polyclonal antibody.

Lane 1: BLNK transfected lysate(50.50 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu, Rt E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

B-cell linker protein / BLNK antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between NCK1 and BLNK. HeLa cells were stained with anti-NCK1 rabbit purified polyclonal 1:1200 and anti-BLNK mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG2a 1D7 Purified Hu, Rt E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

B-cell linker protein / BLNK antibody

BLNK polyclonal antibody ( Cat # PAB6035 ) staining ( 4 µg/ml ) of Daudi lysate ( RIPA buffer, 30 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

B-cell linker protein / BLNK antibody

Western Blotting analysis ( reducing conditions ) of whole cell lysate of Sp2/0 mouse myeloma cell line using BLNK polyclonal antibody ( Cat # PAB13541 ). Rabbit Hu, Ms WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

B-cell linker protein / BLNK pTyr96 antibody

Immunohistochemistry analysis of paraffin-embedded human lymph node, using BLNK (Phospho-Tyr96) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P, WB
50 µg / €205.00
  Assay Biotech

B-cell linker protein / BLNK pTyr96 antibody

Immunohistochemistry analysis of paraffin-embedded human lymph node, using BLNK (Phospho-Tyr96) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P, WB
0.1 mg / €310.00
  Assay Biotech

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