B-Raf proto-oncogene antibody

Principal name

B-Raf proto-oncogene antibody

Alternative names for B-Raf proto-oncogene antibody

BRAF, BRAF1, RAFB1, p94, B-Raf proto-oncogene serine/threonine-protein kinase, v-Raf murine sarcoma viral oncogene homolog B1

SwissProt ID

P15056 (Human), P28028 (Mouse)

Gene ID

673 (BRAF), 109880 (Braf)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey), Can (Canine), Chk (Chicken), Ze (Zebrafish)

Available hosts

Mouse, Rabbit, Chicken

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Paraffin Sections (P), Immunoprecipitation (IP), Dot blot (Dot), Frozen Sections (C), Luminex (LMNX)

Background of B-Raf proto-oncogene antibody

Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. Raf-A, a second member of the Raf gene family of serine/threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of Raf-B is highly restricted with highest levels in the cerebrum and testis.

General readings

Brennan D.F., et al. (2011) Nature 472:366-369

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

B-Raf proto-oncogene antibody

Immunohistochemical staining of paraffin-embedded thyroid tissue within the normal limits using anti-BRAFmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500429, Dilution 1:50) Mouse IgG2a OTI4A5 Purified Hu E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

B-Raf proto-oncogene antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-BRAF mouse monoclonal antibody. (TA500431) Mouse IgG1 OTI5A9 Purified Hu, Mky F, ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

B-Raf proto-oncogene antibody

Immunoprecipitation(IP) of BRAF by using TrueMab monoclonal anti-BRAF antibodies (Negative control: IP without adding anti-BRAF antibody.). For each experiment, 500ul of DDK tagged BRAF overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-BRAF antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody. Mouse IgG2a OTI4B2 Purified Hu, Mky E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

B-Raf proto-oncogene antibody

Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using anti-BRAFmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500439, Dilution 1:50) Mouse IgG2a OTI1G6 Purified Hu, Mky ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

B-Raf proto-oncogene antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-BRAFmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500440, Dilution 1:50) Mouse IgG2a OTI3A7 Purified Can, Hu, Mky, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

B-Raf proto-oncogene antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-BRAF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500441) Mouse IgG2b OTI3E8 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

B-Raf proto-oncogene antibody

Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-BRAF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500444, Dilution 1:50) Mouse IgG1 OTI4C5 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

B-Raf proto-oncogene antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-BRAF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500445, Dilution 1:50) Mouse IgG1 OTI4G6 Purified Hu, Mky P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

B-Raf proto-oncogene antibody

Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-BRAFmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500446, Dilution 1:50) Mouse IgG1 OTI3B12 Purified Can, Hu, Mky P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

B-Raf proto-oncogene antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-BRAFmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500447, Dilution 1:50) Mouse IgG2b OTI1G8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

B-Raf proto-oncogene antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-BRAF mouse monoclonal antibody. (TA500458) Mouse IgG1 OTI3C3 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

B-Raf proto-oncogene antibody

Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-BRAF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500462, Dilution 1:50) Mouse IgG1 OTI4C11 Purified Can, Hu E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

B-Raf proto-oncogene antibody

Anti-BRAF mouse monoclonal antibody (TA500844) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BRAF(RC211013). Mouse IgG1 OTI1D2 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

B-Raf proto-oncogene antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-BRAFmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500845, Dilution 1:50) Mouse IgG1 OTI1E2 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

B-Raf proto-oncogene antibody

Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-BRAFmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500852, Dilution 1:50) Mouse IgG1 OTI1D4 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

B-Raf proto-oncogene antibody

Immunohistochemical analysis of paraffin-embedded human testis tissues using BRAF antibody Cat.-No AM06152SU-N Mouse IgG1 1H12F1 Ascites Hu E, P, WB
0.1 ml / €480.00
  Acris Antibodies GmbH

B-Raf proto-oncogene antibody

Immunohistochemistry-Paraffin: B Raf Antibody (1H12F1, 1H12G10,1F12F11C9) [NBP1-47555] - Immunohistochemical analysis of paraffin-embedded human bladder carcinoma tissue(left) and lung carcinoma tissue(right) showing cytoplasmic localization using anti-BRAF with DAB staining. Mouse IgG1 1H12F1, 1H12G10,1F12F11C9 Purified Hu C, E, P, WB
0.1 ml / €510.00
  Novus Biologicals Inc.

B-Raf proto-oncogene antibody

Immunohistochemistry-Paraffin: B Raf Antibody (5A9) [NBP1-47668] - Immunohistochemical staining of paraffin-embedded lung tissue using anti-BRAFmouse monoclonal antibody. (Dilution 1:50) Mouse 5A9 Aff - Purified Hu, Mky F, ICC/IF, P, WB
0.1 ml / €620.00
  Novus Biologicals Inc.

B-Raf proto-oncogene (Center) antibody

Western blot analysis of BRAF Antibody (Center) polyclonal antibody(Cat.#TA302165)(arrow). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BRAF gene (Lane 2) (Origene Technologies). Rabbit Ig Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

B-Raf proto-oncogene (Center) antibody

Western blot analysis of B-raf in human brain tissue lysate with B-raf antibody at (A) 1 and (B) 2 ug/mL. Chicken Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

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