B3GNT2 / B3GALT7 antibody
Principal name
B3GNT2 / B3GALT7 antibody
Alternative names for B3GNT2 / B3GALT7 antibody
BGnT-1, Beta-1,3-GalTase 7, Beta3Gal-T7, b3Gal-T7, B3GNT1, UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2, Beta3Gn-T1, Beta-1,3-galactosyltransferase 7, Beta-3-Gx-T7, UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase 7, UDP-Gal:beta-GlcNAc beta-1,3-galactosyltransferase 7, UDP-Gal:beta-GlcNAc beta-1,3-galactosyltransferase 7
Gene ID
10678 (B3GNT2)
Ncbi ID
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)
Background of B3GNT2 / B3GALT7 antibody
This gene encodes a member of the beta-1,3-N-acetylglucosaminyltransferase family. This enzyme is a type II transmembrane protein. It prefers the substrate of lacto-N-neotetraose, and is involved in the biosynthesis of poly-N-acetyllactosamine chains. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
General readings
Hayashi N, Nakamori S, Okami J, Nagano H, Dono K, Umeshita K, Sakon M, Narimatsu H, Monden M.
Association between expression levels of CA 19-9 and N-acetylglucosamine-beta;1,3-galactosyltransferase 5 gene in human pancreatic cancer tissue.
Pathobiology. 2004;71(1):26-34.
PMID: 14555842.
AP16681PU-N (0.1µg/ml) staining of Human Pancreas lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot analysis of B3GNT1 expression in transfected 293T cell line by B3GNT1 monoclonal antibody (M05), clone 1A8.Lane 1: B3GNT1 transfected lysate(46.022 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged B3GNT1 is approximately 0.3ng/ml as a capture antibody.
Western Blot: B3GNT2 Lysate [NBL1-07884] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for B3GNT2
NBP1-00158(0.1ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: B3GNT2 Antibody [NBP1-88951] - Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells
Immunofluorescence: B3GNT2 Antibody [NBP1-88951] Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8 | Lane 2: RT-4 | Lane 3: U-251 MG | Lane 4: Human Plasma | Lane 5: Liver | Lane 6: Tonsil
B3GNT2 polyclonal antibody ( Cat # PAB7522 ) ( 0.1 µg/ml ) staining of human pancreas lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
GTX88963 (0.1µg/ml) staining of Human Pancreas lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Anti-B3GNT2 mouse monoclonal antibody (TA505331) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY B3GNT2(RC208100).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY B3GNT2 (RC208100, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-B3GNT2.
Western blot analysis of extracts (10ug) from 8 Human tissue by using anti-B3GNT2 monoclonal antibody at 1:200 (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).
Immunofluorescent staining of HeLa cells using anti-B3GNT2 mouse monoclonal antibody (TA505270).
Anti-B3GNT2 mouse monoclonal antibody (TA505270) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY B3GNT2(RC208100).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY B3GNT2 (RC208100, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-B3GNT2.
HEK293T cells transfected with either RC208100 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-B3GNT2 antibody(TA505270), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-B3GNT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505283)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-B3GNT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505283)
Anti-B3GNT2 mouse monoclonal antibody (TA505283) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY B3GNT2(RC208100).
Immunofluorescent staining of HeLa cells using anti-B3GNT2 mouse monoclonal antibody (TA505283).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY B3GNT2 (RC208100, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-B3GNT2.
- Quick-Filters:
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA505270 | B3GNT2 / B3GALT7 antibody |
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Mouse | IgG2a | OTI1G10 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody!
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| TA505283 | B3GNT2 / B3GALT7 antibody |
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Mouse | IgG2a | OTI3A9 | Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
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| TA303284 | B3GNT2 / B3GALT7 antibody |
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 |
Goat | Aff - Purified | Hu, Ms, Rt | WB |
0.1 mg /
€325.00
|
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| OriGene Technologies, Inc. | |||||||
| TA505331 | B3GNT2 / B3GALT7 antibody |
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Mouse | IgG2a | OTI4B9 | Purified | Hu | ICC/IF, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| H00010678-A01 | B3GNT2 / B3GALT7 antibody |
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Mouse | Serum | Hu | E, WB |
50 µl /
€290.00
|
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| Abnova Taiwan Corp. | |||||||
| H00010678-M05 | B3GNT2 / B3GALT7 antibody |
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Mouse | IgG2a | 1A8 | Purified | Hu | E, WB |
0.1 mg /
€370.00
|
| Abnova Taiwan Corp. | |||||||
| +1 additional image |
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| PAB7522 | B3GNT2 / B3GALT7 antibody |
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Goat | Hu | E, WB |
0.1 mg /
€400.00
|
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| Abnova Taiwan Corp. | |||||||
| NBP1-00158 | B3GNT2 / B3GALT7 antibody |
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Goat | IgG | Aff - Purified | Hu | E, WB |
0.1 mg /
€400.00
|
|
| Novus Biologicals Inc. | |||||||
| NBP1-31915 | B3GNT2 / B3GALT7 antibody |
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Rabbit | IgG | Hu | WB |
0.1 ml /
€340.00
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| Novus Biologicals Inc. | |||||||
| NBP1-88951 | B3GNT2 / B3GALT7 antibody |
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![Immunohistochemistry-Paraffin: B3GNT2 Antibody [NBP1-88951] - Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/B3GNT2 Antibody-Immunohistochemistry-Paraffin-NBP1-88951-0005.jpg) |
Rabbit | IgG | Aff - Purified | Hu | P, WB |
0.1 ml /
€500.00
|
|
| Novus Biologicals Inc. | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP308100 | B3GNT2 / B3GALT7 |
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€680.00
|
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| OriGene Technologies, Inc. | |||||
| H00010678-P01 | B3GNT2 / B3GALT7 |
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Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. |
2 µg /
€300.00
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| Abnova Taiwan Corp. | |||||
| H00010678-Q01-10 | B3GNT2 / B3GALT7 |
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Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. |
10 µg /
€380.00
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| Abnova Taiwan Corp. | |||||
| H00010678-Q01-25 | B3GNT2 / B3GALT7 |
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Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. |
25 µg /
€510.00
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| Abnova Taiwan Corp. | |||||
Lysates (Overexpressed)
| Catalog No. | ||
|---|---|---|
| LY416552 | B3GNT2 overexpression lysate |
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0.1 mg /
€280.00
|
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| OriGene Technologies, Inc. |
