B3GNT2 / B3GALT7 antibody
Principal name
B3GNT2 / B3GALT7 antibody
Alternative names for B3GNT2 / B3GALT7 antibody
BGnT-1, Beta-1,3-GalTase 7, Beta3Gal-T7, b3Gal-T7, B3GNT1, UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2, Beta3Gn-T1, Beta-1,3-galactosyltransferase 7, Beta-3-Gx-T7, UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase 7, UDP-Gal:beta-GlcNAc beta-1,3-galactosyltransferase 7, UDP-Gal:beta-GlcNAc beta-1,3-galactosyltransferase 7
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of B3GNT2 / B3GALT7 antibody
- Kit Component:
- - KN208100G1, B3GNT1 gRNA vector 1 in pCas-Guide vector
- KN208100G2, B3GNT1 gRNA vector 2 in pCas-Guide vector
- KN208100D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Hayashi N, Nakamori S, Okami J, Nagano H, Dono K, Umeshita K, Sakon M, Narimatsu H, Monden M.
Association between expression levels of CA 19-9 and N-acetylglucosamine-beta;1,3-galactosyltransferase 5 gene in human pancreatic cancer tissue.
Pathobiology. 2004;71(1):26-34.
PMID: 14555842.
Western Blot analysis of B3GNT1 expression in transfected 293T cell line by B3GNT1 monoclonal antibody (M05), clone 1A8.Lane 1: B3GNT1 transfected lysate(46.022 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged B3GNT1 is approximately 0.3ng/ml as a capture antibody.
B3GNT2 polyclonal antibody ( Cat # PAB7522 ) ( 0.1 µg/ml ) staining of human pancreas lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
Immunofluorescent staining of HeLa cells using anti-B3GNT2 mouse monoclonal antibody (TA505270).
Anti-B3GNT2 mouse monoclonal antibody (TA505270) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY B3GNT2(RC208100).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY B3GNT2 (RC208100, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-B3GNT2.
HEK293T cells transfected with either RC208100 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-B3GNT2 antibody(TA505270), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-B3GNT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505283)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-B3GNT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505283)
Anti-B3GNT2 mouse monoclonal antibody (TA505283) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY B3GNT2(RC208100).
Immunofluorescent staining of HeLa cells using anti-B3GNT2 mouse monoclonal antibody (TA505283).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY B3GNT2 (RC208100, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-B3GNT2.
Anti-B3GNT2 mouse monoclonal antibody (TA505331) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY B3GNT2(RC208100).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY B3GNT2 (RC208100, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-B3GNT2.
Western blot analysis of extracts (10ug) from 8 Human tissue by using anti-B3GNT2 monoclonal antibody at 1:200 (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).
TA303284 (0.1µg/ml) staining of Human Pancreas lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Quick-Filters:
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA505270 | B3GNT2 / B3GALT7 antibody |
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| B3GNT2 mouse monoclonal antibody, clone OTI1G10 (formerly 1G10) | |||||||
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Mouse | IgG2a | OTI1G10 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody!
|
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| TA505283 | B3GNT2 / B3GALT7 antibody |
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| B3GNT2 mouse monoclonal antibody, clone OTI3A9 (formerly 3A9) | |||||||
 |
Mouse | IgG2a | OTI3A9 | Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
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| TA303284 | B3GNT2 / B3GALT7 antibody |
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| Goat Polyclonal Antibody against B3GNT2 | |||||||
 |
Goat | Aff - Purified | Hu, Ms, Rt | WB |
0.1 mg /
€325.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA505331 | B3GNT2 / B3GALT7 antibody |
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| B3GNT2 mouse monoclonal antibody, clone OTI4B9 (formerly 4B9) | |||||||
 |
Mouse | IgG2a | OTI4B9 | Purified | Hu | ICC/IF, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN208100 | B3GNT2 - human gene knockout kit via CRISPR |
||
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Human | GFP-Puro |
1 kit /
€1,200.00
|
| OriGene Technologies, Inc. | |||
| KN208100BN | B3GNT2 - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN208100LP | B3GNT2 - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN208100RB | B3GNT2 - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP308100 | B3GNT2 / B3GALT7 |
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| Recombinant protein of human UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 (B3GNT2) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€680.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY416552 | B3GNT2 overexpression lysate |
|
|
0.1 mg /
€280.00
|
|
| OriGene Technologies, Inc. |
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC208100L2V | Lenti ORF particles, B3GNT2 (mGFP-tagged) - Human UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 (B3GNT2), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,050.00
|
|
| OriGene Technologies, Inc. | ||
| RC208100L1V | Lenti ORF particles, B3GNT2 (Myc-DDK tagged) - Human UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 (B3GNT2) , 200 uL, >10^7 TU/mL |
|
|
200 µl /
€940.00
|
|
| OriGene Technologies, Inc. | ||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR206220L2V | Lenti ORF particles, B3gnt2 (GFP-tagged) - Mouse UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 (B3gnt2), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,170.00
|
|
| OriGene Technologies, Inc. | ||
| MR206220L1V | Lenti ORF particles, B3gnt2 (Myc-DDK-tagged) - Mouse UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 (B3gnt2), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,050.00
|
|
| OriGene Technologies, Inc. | ||
