BACH1 antibody

Principal name

BACH1 antibody

Alternative names for BACH1 antibody

HA2303, Transcription regulator protein BACH1, BTB and CNC homolog 1, basic leucine zipper transcription factor 1

SwissProt ID

O14867 (Human), P97302 (Mouse)

Gene ID

571 (BACH1)

Available reactivities

Hu (Human), Ms (Mouse), Mky (Monkey), Rt (Rat), Chimp (Chimpanzee)

Available hosts

Mouse, Rabbit, Chicken

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Flow Cytometry (F)

Background of BACH1 antibody

Members of the small Maf family (MafK, MafF, and MafG) are basic region leucine zipper (bZip) proteins that can function as transcriptional activators or repressors. They dimerize with other proteins and bind DNA to either represse or activate transcription depending on the dimer compositions. BACH1 and BACH2, heterodimerization partners of MafK, are members of a novel family of BTB/POZ-basic region leucine zipper (bzip) factors. BACH1 and BACH2 have significant similarity to each other in BTB domain and Cap‘n’ collar-type bZip domain but are otherwise divergent. BACH1 appears ubiquitous, whereas BACH2 is restricted to monocytes, neuronal cells and is abundantly expressed in the early stages of B-cell differentiation. BACH2, a 841 amino acid polypeptide, is an Nrf2-related transcription repressor and a tissue-specific partner of the Maf oncoprotein family. In culture cells, BACH2 is localized to the cytoplasm through its C-terminal cytoplasmic localization signal (CLS). Oxidative stressors aborted the CLS activity and induce nuclear accumulation of BACH2, which mediates nucleocytoplasmic communciation to couple oxidative stress and transcription repression in mammalian cells. BACH2 heterodimerizes with MAZR through its BTB/POZ domain to activate transcription. BACH2 also plays an important role in the regulation of B cell development.

General readings

Litman,R., et al., (2005) Cancer Cell 8 (3), 255-265

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

BACH1 antibody

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-BACH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504484) Mouse IgG1 OTI4E11 Purified Hu F, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

BACH1 antibody

Western blot analysis of extracts from HeLa cells, HepG2 cells and 293 cells, using BACH1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

BACH1 antibody

WB Suggested Anti-BACH1 Antibody Titration: 1.25 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Jurkat cell lysate Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BACH1 antibody

Western blot (WB) analysis of BACH1 antibody (Cat.-No.: AP06634PU-N) in extracts from HeLa cells. Rabbit Aff - Purified Hu ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

BACH1 (Isoform 1) (92-104) antibody

Skeletal muscle: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu, Mky, Ms, Rt E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

BACH1 (C-term) antibody

BACH1 Antibody (C-term) (Cat. #AP50333PU-N) western blot analysis in ZR-75-1 cell line lysates (35µg/lane).This demonstrates the BACH1 antibody detected the BACH1 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

BACH1 antibody

BACH1 Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

BACH1 antibody

BACH1 monoclonal antibody (M02), clone 1B8 Western Blot analysis of BACH1 expression in NIH/3T3 ( Cat # L018V1 ). Mouse IgG2a 1B8 Purified Hu, Ms E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

BACH1 antibody

Western blot using BACH1 polyclonal antibody ( Cat # PAB10027 ) shows detection of a band at~105 kDa ( lane 1 ) corresponding to human BACH1 present in a 293 whole cell lysate ( arrowhead ).
Lane 2 shows that specific band staining is competed out when the antibody is pre-incubated with the peptide immunogen prior to reaction.
Approximately 35 µg of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose.
After blocking the membrane was probed with the primary antibody diluted to 1:1,000.
Reaction occurred 2 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDyeTM800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at roomtemperature (800 nm channel, green).
Molecular weight estimation was made by comparison to prestained MW markers in lane M ( 700 nm channel, red ).
IRDyeTM800 fluorescence image was capturedusing the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR,Inc. Chimp, Hu
0.1 mg / €560.00
  Abnova Taiwan Corp.

BACH1 antibody

Immunofluorescence analysis of HeLa cells, using BACH1 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, ICC/IF, WB
50 µg / €205.00
  Assay Biotech

BACH1 antibody

Immunofluorescence analysis of HeLa cells, using BACH1 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, ICC/IF, WB
0.1 mg / €310.00
  Assay Biotech

BACH1 antibody

Human Jurkat; WB Suggested Anti-BACH1 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:1562500. Positive Control: Jurkat cell lysate; BACH1 antibody - C-terminal region (ARP38012_T100) in Human Jurkat cells using Western Blot Rabbit Aff - Purified Hu WB
0.1 mg / €310.00
  AVIVA Systems Biology

BACH1 antibody

Immunohistochemical analysis of BACH1 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Mky ICC/IF, IP, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

BACH1 antibody

Immunohistochemical analysis of BACH1 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Mky ICC/IF, IP, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

BACH1 antibody

Immunohistochemical analysis of BACH1 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Mky ICC/IF, IP, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

BACH1 antibody

BACH1 Rabbit IgG Aff - Purified Hu WB
50 µg / €680.00
  Funakoshi Co., Ltd.

BACH1 antibody

Western Blot: BACH1 Antibody [NB100-92282] - Western blot (WB) analysis of BACH1 (L160)  antibody in extracts from HeLa cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

BACH1 antibody

BACH1 Rabbit IgG Aff - Purified Hu E, ICC/IF, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

BACH1 antibody

BACH1 Rabbit IgG Aff - Purified Hu E, ICC/IF, WB
50 µg / €290.00
  Novus Biologicals Inc.

BACH1 antibody

Western Blot: BACH1 Antibody [NBP1-71814]-Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Affinity purified rabbit anti-BACH1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate.  BACH1 was also immunoprecipitated by rabbit anti-BACH1 antibody NBP1-71815 which recognizes a downstream epitope. Rabbit Aff - Purified Hu IP, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

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