BACH2 antibody

Principal name

BACH2 antibody

Alternative names for BACH2 antibody

Transcription regulator protein BACH2, BTB and CNC homolog 2, basic leucine zipper transcription factor 2

SwissProt ID

P97303 (Mouse), Q9BYV9 (Human)

Gene ID

60468 (BACH2), 12014

Available reactivities

Bov (Bovine), Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Enzyme Immunoassay (E), Immunoprecipitation (IP)

Background of BACH2 antibody

Members of the small Maf family (MafK, MafF, and MafG) are basic region leucine zipper (bZip) proteins that can function as transcriptional activators or repressors. They dimerize with other proteins and bind DNA to either represse or activate transcription depending on the dimer compositions. BACH1 and BACH2, heterodimerization partners of MafK, are members of a novel family of BTB/POZ-basic region leucine zipper (bzip) factors. BACH1 and BACH2 have significant similarity to each other in BTB domain and Cap ‘n’ collar-type bZip domain but are otherwise divergent. BACH1 appears ubiquitous, whereas BACH2 is restricted to monocytes, neuronal cells and is abundantly expressed in the early stages of B-cell differentiation. BACH2, a 841 amino acid polypeptide, is an Nrf2-related transcription repressor and a tissue-specific partner of the Maf oncoprotein family. In culture cells, BACH2 is localized to the cytoplasm through its C-terminal cytoplasmic localization signal (CLS). Oxidative stressors aborted the CLS activity and induce nuclear accumulation of BACH2, which mediates nucleocytoplasmic communciation to couple oxidative stress and transcription repression in mammalian cells. BACH2 heterodimerizes with MAZR through its BTB/POZ domain to activate transcription. BACH2 also plays an important role in the regulation of B cell development.

General readings

Hong,S.W., (2008) Biochem. Biophys. Res. Commun. 365 (3), 426-432

13 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

BACH2 antibody

Western Blot of Rabbit anti-Bach2 antibody. Lane 1: 293T cell lysates overexpressing Bach2-Flag. Lane 2: 293T cell lysates. Load: 20 µg per lane. Primary antibody: Bach-2 antibody at 1:1000 for overnight at 4°C. Secondary antibody: rabbit HRP secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 91.7 kDa, ~130 kDa for Bach2. Other band(s): none. Rabbit IgG Purified Ms E, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BACH2 antibody

WB Suggested Anti-BACH2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Thymus Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

BACH2 antibody

WB Suggested Anti-BACH2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: 293T cell lysate; BACH2 is supported by BioGPS gene expression data to be expressed in HEK293T Rabbit IgG Purified Bov, Eq, GP, Hu, Ms, Rb, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

BACH2 (Center) antibody

BACH2 Antibody (Center) (Cat. #AP50334PU-N) western blot analysis in K562 cell line lysates (35µg/lane).This demonstrates the BACH2 antibody detected the BACH2 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

BACH2 (C-term) antibody

BACH2 Antibody (C-term) (Cat. #AP50335PU-N) western blot analysis in MDA-MB435 cell line lysates (35µg/lane).This demonstrates the BACH2 antibody detected the BACH2 protein (arrow). Rabbit Ig Aff - Purified Hu F, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

BACH2 antibody

Brain (cortex) stained with BACH2 antibody Cat.-No AP55330PU-N in Immunohistochemistry on Paraffin Sections Rabbit Purified Hu WB
0.1 mg / €420.00
  Acris Antibodies GmbH

BACH2 antibody

BACH2 Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

BACH2 antibody

Human Thymus; WB Suggested Anti-BACH2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Thymus; BACH2 antibody - middle region (ARP33233_P050) in Human Thymus cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

BACH2 antibody

Human Tonsil; BACH2 antibody - middle region (ARP39513_P050) in Human Tonsil cells using Immunohistochemistry Rabbit Aff - Purified Bov, Hu, Ms, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

BACH2

BACH2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

BACH2

BACH2 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

BACH2

BACH2 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

BACH2 overexpression lysate

BACH2 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

13 Item(s)

per page
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