BAG1 antibody

Principal name

BAG1 antibody

Alternative names for BAG1 antibody

BAG-1, HAP, RAP46, BAG family molecular chaperone regulator 1, Bcl-2-associated athanogene 1, Glucocorticoid receptor-associated protein RAP46

SwissProt ID

Q60739 (Mouse), Q99933 (Human)

Gene ID

573 (BAG1), 12017 (Bag1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), ELISA (detection) (E(detection)), Immunoprecipitation (IP)

Background of BAG1 antibody

Bag1 belongs to the Bcl-2 associated athanogene (BAG) family of multifunctiol proteins and was the first of six related proteins isolated from humans. This widely expressed protein interacts with a number of sigling molecules (including Bcl2, HGF receptor and Raf1) as it regulates sigling molecules in pathways involving cell survival, growth and differentiation. The most common role played by Bag1 protein is as an inhibitor of proteins favoring apoptosis. Bag1 also plays a role in Raf1 sigling and binds D as a transcription activator (4). Bag1 protein is a well-characterized inhibitor of its binding partner HSP70. A conserved carboxy-termil BAG domain within Bag1 interacts with the ATPase domain of HSP70 to negatively regulate heat shock protein chaperone activity. The multiple isoforms of Bag1 protein generated from a single transcript share a common ubiquitin homology domain and a carboxy-termil Hsp70 binding region but differ in length and cellular localization. The 50 kDa long (Bag1-L) isoform also contains a nuclear localization sigl and is often found in the nucleus where it activates transcription. The 46 kDa intermediate (Bag1-M) isoform is found mainly in the cytoplasm but can also translocate to the nucleus when associated with other proteins. The shorter 29-33 kDa isoforms (Bag1-S, Bag-1) isoforms are found primarily in the cytoplasm. High expression of the anti-apoptotic Bag1 protein correlates with increased survival in patients with particular forms of cancer, leading researchers to study possible therapeutic roles for Bag1 protein.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

BAG1 antibody

Western blot analysis of extracts of NCI-H460 cell lines, using BAG1 antibody. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, P
0.1 mg / €325.00
  OriGene Technologies, Inc.

BAG1 antibody

Immunoperoxidase of monoclonal antibody to BAG1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml] Mouse IgG2a 2D3 Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

BAG1 antibody

Western blot analysis of BAG-1 in PC-3 cell lysate with BAG-1 antibody at (A) 1 and (B) 2 µg/ml. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BAG1 antibody

Western blot analysis of BAG-1 in PC-3 cell lysate with BAG-1 antibody at (A) 1 and (B) 2 ug/ml. Rabbit IgG Purified Hu, Ms ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BAG1 antibody

Predicted band size: 39 kDa . Positive control: Hela and Raji cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lane1: Hela cell lysate Lane2: Raji cell lysate Lysates: 50 ug per lane Primary antibody: 1/350 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 5 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

BAG1 antibody

Predicted band size: 39 kDa . Positive control: Hela, K562 and Raji cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lane1: Hela cell lysate Lane2: K562 cell lysate Lane3: Raji cell lysate Lysates: 50 ug per lane Primary antibody: 1/750 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

BAG1 antibody

Western blot analysis of extracts from CHO-K1 cells, transfected with a human pEGFP-C1-BAG1 construct (A) or transfected with a human pEGFP-C1 construct (B), using Bag1 mouse mAb (1:1000 diluted). Mouse IgG1 2C12-A8-1D7 Purified WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

BAG1 antibody

Western blot analysis of Bag 1 expression in HeLa cell lysate Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

BAG1 (full length) antibody

Western blot analysis of Bag-1 expression in HeLa (1), HL-60 (2), WR19L (3) and PC12 (4) using AM26586AF-N. Mouse IgG1 4A2 Azide Free Hu, Ms, Rt WB
0.1 mg / €360.00
  Acris Antibodies GmbH

BAG1 (C-term) antibody

Western blot analysis in whole cell lysate from PC-3 human prostate cancer cells probed with Rabbit anti Human BAG1 (AP05585PU-N) at 1(A) and 2(B) µg/ml. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €420.00
  Acris Antibodies GmbH

BAG1 (N-term) antibody

Immunohistochemical staining of human lymph node with Rabbit anti Human BAG1 (AP05586PU-N) Rabbit IgG Purified Hu, Ms WB
0.1 mg / €330.00
  Acris Antibodies GmbH

BAG1 antibody

Kidney: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

BAG1 (N-term) antibody

Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Adrenal. Rabbit Purified Hu, Ms P, WB
50 µg / €470.00
  Acris Antibodies GmbH

BAG1 (C-term) antibody

Western blot analysis of BAG1 Antibody (C-term) (AP17147PU-N) in MDA-MB231 cell line lysates (35 µg/lane). BAG1 (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

BAG1 antibody

BAG1 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

BAG1 antibody

Western Blot analysis of BAG1 expression in transfected 293T cell line (H00000573-T01) by BAG1 MaxPab polyclonal antibody.

Lane 1: BAG1 transfected lysate(30.14 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

BAG1 antibody

BAG1 MaxPab rabbit polyclonal antibody. Western Blot analysis of BAG1 expression in human placenta. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

BAG1 antibody

Immunofluorescence of monoclonal antibody to BAG1 on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG2a 4E2 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

BAG1 antibody

BAG1 Mouse IgG2a 2D3 Ascites Hu, Ms, Rt E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

BAG1 antibody

Immunoperoxidase of monoclonal antibody to BAG1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml] Mouse IgG 4C10 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

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